Table 2.

Oligonucleotides used in this study

No.NameSequence (5′ to 3′)
1 S22A_Fw 5′-GCT​CCA​CTC​CGC​TGG​CAC​CCA​CCC​GCA​TCA​CC-3′ 
2 S22A_Rv 3′-GGT​GAT​GCG​GGT​GGG​TGC​CAG​CGG​AGT​GGA​GC-5′ 
3 S22D_Fw 5′-GCT​CCA​CTC​CGC​TGG​ACC​CCA​CCC​GCA​TCA​CC-3′ 
4 S22D_Rv 3′-GGT​GAT​GCG​GGT​GGG​GTC​CAG​CGG​AGT​GGA​GC-5′ 
5 Q432X_Fw 5′-CAG​CAG​CTT​CTC​ATA​GCA​CGC​ACG​CAC-3′ 
6 Q432X_Rv 3′-GTG​CGT​GCG​TGC​TAT​GAG​AAG​CTG​CTG-5′ 
7 R435C_Fw 5′-CTC​ACA​GCA​CGC​ATG​CAC​TAG​CGG​GC-3′ 
8 R435C_Rv 3′-GCC​CGC​TAG​TGC​ATG​CGT​GCT​GTG​AG-5′ 
9 R453W_Fw 5′-GGC​AAG​TTT​GTC​TGG​CTG​CGC​AAC​AAG-3′ 
10 R453W_Rv 3′-CTT​GTT​GCG​CAG​CCA​GAC​AAA​CTT​GCC-5′ 
11 R471C_Fw 5′-CAA​TTG​GCA​GAT​CAA​GTG​CCA​GAA​TGG​AG-3′ 
12 R471C_Rv 3′-CTC​CAT​TCT​GGC​ACT​TGA​TCT​GCC​AAT​TG-5′ 
13 R482W_Fw 5′-CTT​GCT​GAC​TTA​CTG​GTT​CCC​ACC​AAA​G-3′ 
14 R482W_Rv 3′-CTT​TGG​TGG​GAA​CCA​GTA​AGT​CAG​CAA​G-5′ 
15 R527H_Fw 5′-GGA​ACA​GCC​TGC​ATA​CGG​CTC​TCA​TC-3′ 
16 R527H_Rv 3′-GAT​GAG​AGC​CGT​ATG​CAG​GCT​GTT​CC-5′ 
17 A529V_Fw 5′-GCC​TGC​GTA​CGG​TTC​TCA​TCA​ACT​C-3′ 
18 A529V_Rv 3′-GAG​TTG​ATG​AGA​ACC​GTA​CGC​AGG​C-5′ 
19 K542N_Fw 5′-GGC​CAT​GCG​CAA​CCT​GGT​GCG​CTC​AG-3′ 
20 K542N_Rv 3′-CTG​AGC​GCA​CCA​GGT​TGC​GCA​TGG​CC-5′ 
21 V567X_Fw (Δ6aa) 5′-CCA​TCA​CCA​CCA​CTA​GAG​TGG​TAG​CCG​C-3′ 
22 V567X_Rv (Δ6aa) 3′-GCG​GCT​ACC​ACT​CTA​GTG​GTG​GTG​ATG​G-5′ 
23 if_LA-422_Fw (ΔNLS) 5′-GGT​ACC​GCG​GGC​CCG​CAC​GCA​CGC​ACT​AGC​GGG​CGC​G-3′ 
24 if_LA-417_Rv (ΔNLS) 3′-CGG​GCC​CGC​GGT​ACC​GGT​GAC​GCT​GCC​CCC​ACC​CTG-5′ 
25 if_SUN2-NLS_Fw (ΔNLS + NLSSUN2) 5′-GGG​GGC​AGC​GTC​ACC​AAA​GAC​AGT​CCT​CTC​AGG​A-3′ 
26 if_SUN2-NLS_Rv (ΔNLS + NLSSUN2) 3′-GCG​GCT​CTC​AGT​GGA​CAG​GCG​CTT​CAT​GTT​GCT-5′ 
27 inv_LA-422_Fw (ΔNLS + NLSSUN2) 5′-TCC​ACT​GAG​AGC​CGC​AGC​AGC-3′ 
28 inv_LA-417_Rv (ΔNLS + NLSSUN2) 3′-GGT​GAC​GCT​GCC​CCC​ACC​CTG-5′ 
29 if_LB1-IgF_Fw (Δ433-548 + IgFLB1) 5′-AGC​AGC​TTC​TCA​CAG​TCC​GCC​TCA​GCC​ACT​GGA​AAT​G-3′ 
30 if_LB1-IgF_Rv (Δ433-548 + IgFLB1) 3′-GTC​GTC​CTC​AAC​CAC​GGT​TGT​TTT​AAA​GAC​TGT​AC-5′ 
31 inv_LA-548_Fw (Δ433-548 + IgFLB1) 5′-GTG​GTT​GAG​GAC​GAC​GAG​GAT​G-3′ 
32 inv_LA-433_Rv (Δ433-548 + IgFLB1) 3′-CTG​TGA​GAA​GCT​GCT​GCG​GC-5′ 
33 if_BSD_Fw 5′-GCC​ACC​ATG​GCC​AAG​CCT​TTG​TCT​CAA-3′ 
34 if_BSD-WPRE_Rv 3′-TTA​TTC​CTT​TGC​CCT​TAG​CCC​TCC​CAC​ACA​TAA​C-5′ 
35 if_WPRE_Fw 5′-AGG​GCA​AAG​GAA​TAA​GTC​GAC​AAT​CAA​CCT​CTG​GAT​TAC-3′ 
36 if_EF1-BSD_Rv 3′-CTT​GGC​CAT​GGT​GGC​TGC​AGC​TAG​CGT​AGG​CGC​CGG​TC-5′ 
37 if_SV40-NLS_s 5′-GAT​CCG​CTA​GCG​CCA​CCA​TGG​CCC​CAA​AAA​AGA​AGA​GAA​AGG​TTG​TAT​ACC​GGG​CCA​CCA-3′ 
38 if_SV40-NLS_as 5′-TGG​TGG​CCC​GGT​ATA​CAA​CCT​TTC​TCT​TCT​TTT​TTG​GGG​CCA​TGG​TGG​CGC​TAG​CGG​ATC-3′ 
39 BsrGI-mycNLS-XhoI_s 5′-GTA​CAA​GCC​TGC​TGC​CAA​ACG​CGT​TAA​ACT​AGA​CTA​AC-3′ 
40 XhoI-mycNLS-BsrGI_as 5′-TCG​AGT​TAG​TCT​AGT​TTA​ACG​CGT​TTG​GCA​GCA​GGC​TT-3′ 
41 if_pCDH-CMV-GFP_Fw 5′-CAT​AGA​AGA​TTC​TAG​AGC​CAC​CAT​GGT​GAG​CAA​G-3′ 
42 if_pCDH-CMV-GFP_Rv 3′-ATT​CGC​TAG​CTC​TAG​TCT​TGT​ACA​GCT​CGT​CCA​TG-5′ 
43 if_pLKO.6-Zeo_Fw 5′-CTC​CCC​AGG​GGG​ATC​CAT​GGC​CAA​GTT​GAC​CAG​TG-3′ 
44 if_pLKO.6-Zeo_Rv 3′-TCA​TTG​GTC​TTA​AAG​GTA​CCT​CAG​TCC​TGC​TCC​TCG​GC-5′ 
45 if_pLKO.7-sfCherry_Fw 5′-CTC​CCC​AGG​GGG​ATC​CGC​CAC​CAT​GGT​GAG​CAA​G-3′ 
46 if_pLKO.7-sfCherry_Rv 3′-TCA​TTG​GTC​TTA​AAG​GTA​CCT​TAC​TTG​TAC​AGC​TCG​TCC-5′ 
47 shScramble_s 5′-CCG​GCA​ACA​AGA​TGA​AGA​GCA​CCA​ACT​CGA​GTT​GGT​GCT​CTT​CAT​CTT​GTT​GTT​TTT​G-3′ 
48 shScramble_as 5′-AAT​TCA​AAA​ACA​ACA​AGA​TGA​AGA​GCA​CCA​ACT​CGA​GTT​GGT​GCT​CTT​CAT​CTT​GTT​G-3′ 
49 shBanf1#1-TRCN0000124958_Fw 5′-CCG​GTG​GCC​AGT​TTC​TGG​TGC​TAA​ACT​CGA​GTT​TAG​CAC​CAG​AAA​CTG​GCC​ATT​TTT​G-3′ 
50 shBanf1#1-TRCN0000124958_Rv 3′-AAT​TCA​AAA​ATG​GCC​AGT​TTC​TGG​TGC​TAA​ACT​CGA​GTT​TAG​CAC​CAG​AAA​CTG​GCC​A-5′ 
51 shBanf1#2-TRCN0000124955_Fw 5′-CCG​GGA​CAA​GGC​TTA​TGT​GGT​CCT​TCT​CGA​GAA​GGA​CCA​CAT​AAG​CCT​TGT​CTT​TTT​G-3′ 
52 shBanf1#2-TRCN0000124955_Rv 3′-AAT​TCA​AAA​AGA​CAA​GGC​TTA​TGT​GGT​CCT​TCT​CGA​GAA​GGA​CCA​CAT​AAG​CCT​TGT​C-5′ 
53 if_Halo-C1_Fw 5′-CGC​TAG​CGC​TAC​CGG​GCC​ACC​ATG​GCA​GAA​ATC​GGT​ACT​GGC-3′ 
54 if_Halo-C1_Rv 3′-CTT​GAG​CTC​GAG​ATC​TGC​CGG​AAA​TCT​CGA​GCG​TCG​AC-5′ 
55 gbsn_Banf1-1as 5′-CTC​CAG​CCT​CTT​GCT​CAG​GAC​GTC​ACC​AAT​CCC​GGC​CAG​GCT​CCC​CAC​TGG​CTT​TTC​CCC​CAT-​GGG​CTC​TGC​CAC​GAA​GTC​TCG​GTG​CTT​TTG​GGA​GGT​TGT​CAT​AGC​TTG​AGC​TCG​AGA-3′ 
56 gbsn_Banf1-2s 5′-AGC​AAG​AGG​CTG​GAG​GAA​AGG​GGC​TTT​GAC​AAG​GCT​TAT​GTG​GTC​CTT​GGC​CAG​TTT​CTG​GTG​-CTA​AAG​AAA​GAT​GAA​GAC​CTC​TTC​CGA​GAA​TGG​CTG​AAG​GAT​ACA​TGT​GGT​GCC​AAT-3′ 
57 gbsn_Banf1-3as 5′-GCA​GAA​TTC​GAA​GCT​CTA​CAA​GAA​GGC​ATC​ACA​CCA​TTC​TCG​AAG​GCA​CCC​AAA​GCA​GTC​CC-G​GGA​CTG​CTT​GGC​ATT​GGC​ACC​ACA​TGT-3′ 
58 if_h-cGAS_Fw 5′-CGC​TAG​CGC​TAC​CGG​ATG​CAG​CCT​TGG​CAC​GGA-3′ 
59 if_h-cGAS_Rv 3′-GCT​CAC​CAT​GGT​GGC​AAA​TTC​ATC​AAA​AAC​TGG​AAA​C-5′ 
No.NameSequence (5′ to 3′)
1 S22A_Fw 5′-GCT​CCA​CTC​CGC​TGG​CAC​CCA​CCC​GCA​TCA​CC-3′ 
2 S22A_Rv 3′-GGT​GAT​GCG​GGT​GGG​TGC​CAG​CGG​AGT​GGA​GC-5′ 
3 S22D_Fw 5′-GCT​CCA​CTC​CGC​TGG​ACC​CCA​CCC​GCA​TCA​CC-3′ 
4 S22D_Rv 3′-GGT​GAT​GCG​GGT​GGG​GTC​CAG​CGG​AGT​GGA​GC-5′ 
5 Q432X_Fw 5′-CAG​CAG​CTT​CTC​ATA​GCA​CGC​ACG​CAC-3′ 
6 Q432X_Rv 3′-GTG​CGT​GCG​TGC​TAT​GAG​AAG​CTG​CTG-5′ 
7 R435C_Fw 5′-CTC​ACA​GCA​CGC​ATG​CAC​TAG​CGG​GC-3′ 
8 R435C_Rv 3′-GCC​CGC​TAG​TGC​ATG​CGT​GCT​GTG​AG-5′ 
9 R453W_Fw 5′-GGC​AAG​TTT​GTC​TGG​CTG​CGC​AAC​AAG-3′ 
10 R453W_Rv 3′-CTT​GTT​GCG​CAG​CCA​GAC​AAA​CTT​GCC-5′ 
11 R471C_Fw 5′-CAA​TTG​GCA​GAT​CAA​GTG​CCA​GAA​TGG​AG-3′ 
12 R471C_Rv 3′-CTC​CAT​TCT​GGC​ACT​TGA​TCT​GCC​AAT​TG-5′ 
13 R482W_Fw 5′-CTT​GCT​GAC​TTA​CTG​GTT​CCC​ACC​AAA​G-3′ 
14 R482W_Rv 3′-CTT​TGG​TGG​GAA​CCA​GTA​AGT​CAG​CAA​G-5′ 
15 R527H_Fw 5′-GGA​ACA​GCC​TGC​ATA​CGG​CTC​TCA​TC-3′ 
16 R527H_Rv 3′-GAT​GAG​AGC​CGT​ATG​CAG​GCT​GTT​CC-5′ 
17 A529V_Fw 5′-GCC​TGC​GTA​CGG​TTC​TCA​TCA​ACT​C-3′ 
18 A529V_Rv 3′-GAG​TTG​ATG​AGA​ACC​GTA​CGC​AGG​C-5′ 
19 K542N_Fw 5′-GGC​CAT​GCG​CAA​CCT​GGT​GCG​CTC​AG-3′ 
20 K542N_Rv 3′-CTG​AGC​GCA​CCA​GGT​TGC​GCA​TGG​CC-5′ 
21 V567X_Fw (Δ6aa) 5′-CCA​TCA​CCA​CCA​CTA​GAG​TGG​TAG​CCG​C-3′ 
22 V567X_Rv (Δ6aa) 3′-GCG​GCT​ACC​ACT​CTA​GTG​GTG​GTG​ATG​G-5′ 
23 if_LA-422_Fw (ΔNLS) 5′-GGT​ACC​GCG​GGC​CCG​CAC​GCA​CGC​ACT​AGC​GGG​CGC​G-3′ 
24 if_LA-417_Rv (ΔNLS) 3′-CGG​GCC​CGC​GGT​ACC​GGT​GAC​GCT​GCC​CCC​ACC​CTG-5′ 
25 if_SUN2-NLS_Fw (ΔNLS + NLSSUN2) 5′-GGG​GGC​AGC​GTC​ACC​AAA​GAC​AGT​CCT​CTC​AGG​A-3′ 
26 if_SUN2-NLS_Rv (ΔNLS + NLSSUN2) 3′-GCG​GCT​CTC​AGT​GGA​CAG​GCG​CTT​CAT​GTT​GCT-5′ 
27 inv_LA-422_Fw (ΔNLS + NLSSUN2) 5′-TCC​ACT​GAG​AGC​CGC​AGC​AGC-3′ 
28 inv_LA-417_Rv (ΔNLS + NLSSUN2) 3′-GGT​GAC​GCT​GCC​CCC​ACC​CTG-5′ 
29 if_LB1-IgF_Fw (Δ433-548 + IgFLB1) 5′-AGC​AGC​TTC​TCA​CAG​TCC​GCC​TCA​GCC​ACT​GGA​AAT​G-3′ 
30 if_LB1-IgF_Rv (Δ433-548 + IgFLB1) 3′-GTC​GTC​CTC​AAC​CAC​GGT​TGT​TTT​AAA​GAC​TGT​AC-5′ 
31 inv_LA-548_Fw (Δ433-548 + IgFLB1) 5′-GTG​GTT​GAG​GAC​GAC​GAG​GAT​G-3′ 
32 inv_LA-433_Rv (Δ433-548 + IgFLB1) 3′-CTG​TGA​GAA​GCT​GCT​GCG​GC-5′ 
33 if_BSD_Fw 5′-GCC​ACC​ATG​GCC​AAG​CCT​TTG​TCT​CAA-3′ 
34 if_BSD-WPRE_Rv 3′-TTA​TTC​CTT​TGC​CCT​TAG​CCC​TCC​CAC​ACA​TAA​C-5′ 
35 if_WPRE_Fw 5′-AGG​GCA​AAG​GAA​TAA​GTC​GAC​AAT​CAA​CCT​CTG​GAT​TAC-3′ 
36 if_EF1-BSD_Rv 3′-CTT​GGC​CAT​GGT​GGC​TGC​AGC​TAG​CGT​AGG​CGC​CGG​TC-5′ 
37 if_SV40-NLS_s 5′-GAT​CCG​CTA​GCG​CCA​CCA​TGG​CCC​CAA​AAA​AGA​AGA​GAA​AGG​TTG​TAT​ACC​GGG​CCA​CCA-3′ 
38 if_SV40-NLS_as 5′-TGG​TGG​CCC​GGT​ATA​CAA​CCT​TTC​TCT​TCT​TTT​TTG​GGG​CCA​TGG​TGG​CGC​TAG​CGG​ATC-3′ 
39 BsrGI-mycNLS-XhoI_s 5′-GTA​CAA​GCC​TGC​TGC​CAA​ACG​CGT​TAA​ACT​AGA​CTA​AC-3′ 
40 XhoI-mycNLS-BsrGI_as 5′-TCG​AGT​TAG​TCT​AGT​TTA​ACG​CGT​TTG​GCA​GCA​GGC​TT-3′ 
41 if_pCDH-CMV-GFP_Fw 5′-CAT​AGA​AGA​TTC​TAG​AGC​CAC​CAT​GGT​GAG​CAA​G-3′ 
42 if_pCDH-CMV-GFP_Rv 3′-ATT​CGC​TAG​CTC​TAG​TCT​TGT​ACA​GCT​CGT​CCA​TG-5′ 
43 if_pLKO.6-Zeo_Fw 5′-CTC​CCC​AGG​GGG​ATC​CAT​GGC​CAA​GTT​GAC​CAG​TG-3′ 
44 if_pLKO.6-Zeo_Rv 3′-TCA​TTG​GTC​TTA​AAG​GTA​CCT​CAG​TCC​TGC​TCC​TCG​GC-5′ 
45 if_pLKO.7-sfCherry_Fw 5′-CTC​CCC​AGG​GGG​ATC​CGC​CAC​CAT​GGT​GAG​CAA​G-3′ 
46 if_pLKO.7-sfCherry_Rv 3′-TCA​TTG​GTC​TTA​AAG​GTA​CCT​TAC​TTG​TAC​AGC​TCG​TCC-5′ 
47 shScramble_s 5′-CCG​GCA​ACA​AGA​TGA​AGA​GCA​CCA​ACT​CGA​GTT​GGT​GCT​CTT​CAT​CTT​GTT​GTT​TTT​G-3′ 
48 shScramble_as 5′-AAT​TCA​AAA​ACA​ACA​AGA​TGA​AGA​GCA​CCA​ACT​CGA​GTT​GGT​GCT​CTT​CAT​CTT​GTT​G-3′ 
49 shBanf1#1-TRCN0000124958_Fw 5′-CCG​GTG​GCC​AGT​TTC​TGG​TGC​TAA​ACT​CGA​GTT​TAG​CAC​CAG​AAA​CTG​GCC​ATT​TTT​G-3′ 
50 shBanf1#1-TRCN0000124958_Rv 3′-AAT​TCA​AAA​ATG​GCC​AGT​TTC​TGG​TGC​TAA​ACT​CGA​GTT​TAG​CAC​CAG​AAA​CTG​GCC​A-5′ 
51 shBanf1#2-TRCN0000124955_Fw 5′-CCG​GGA​CAA​GGC​TTA​TGT​GGT​CCT​TCT​CGA​GAA​GGA​CCA​CAT​AAG​CCT​TGT​CTT​TTT​G-3′ 
52 shBanf1#2-TRCN0000124955_Rv 3′-AAT​TCA​AAA​AGA​CAA​GGC​TTA​TGT​GGT​CCT​TCT​CGA​GAA​GGA​CCA​CAT​AAG​CCT​TGT​C-5′ 
53 if_Halo-C1_Fw 5′-CGC​TAG​CGC​TAC​CGG​GCC​ACC​ATG​GCA​GAA​ATC​GGT​ACT​GGC-3′ 
54 if_Halo-C1_Rv 3′-CTT​GAG​CTC​GAG​ATC​TGC​CGG​AAA​TCT​CGA​GCG​TCG​AC-5′ 
55 gbsn_Banf1-1as 5′-CTC​CAG​CCT​CTT​GCT​CAG​GAC​GTC​ACC​AAT​CCC​GGC​CAG​GCT​CCC​CAC​TGG​CTT​TTC​CCC​CAT-​GGG​CTC​TGC​CAC​GAA​GTC​TCG​GTG​CTT​TTG​GGA​GGT​TGT​CAT​AGC​TTG​AGC​TCG​AGA-3′ 
56 gbsn_Banf1-2s 5′-AGC​AAG​AGG​CTG​GAG​GAA​AGG​GGC​TTT​GAC​AAG​GCT​TAT​GTG​GTC​CTT​GGC​CAG​TTT​CTG​GTG​-CTA​AAG​AAA​GAT​GAA​GAC​CTC​TTC​CGA​GAA​TGG​CTG​AAG​GAT​ACA​TGT​GGT​GCC​AAT-3′ 
57 gbsn_Banf1-3as 5′-GCA​GAA​TTC​GAA​GCT​CTA​CAA​GAA​GGC​ATC​ACA​CCA​TTC​TCG​AAG​GCA​CCC​AAA​GCA​GTC​CC-G​GGA​CTG​CTT​GGC​ATT​GGC​ACC​ACA​TGT-3′ 
58 if_h-cGAS_Fw 5′-CGC​TAG​CGC​TAC​CGG​ATG​CAG​CCT​TGG​CAC​GGA-3′ 
59 if_h-cGAS_Rv 3′-GCT​CAC​CAT​GGT​GGC​AAA​TTC​ATC​AAA​AAC​TGG​AAA​C-5′ 

See Materials and methods. Oligonucleotides were synthesized by Integrated DNA Technologies (IDT) or Sigma-Aldrich.

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