Antibodies and reagents
| Name . | Company, product number, host . | Dilution . |
|---|---|---|
| FLAG M2 | Sigma F3165, mouse | 1:1,000 |
| Rap2 | BD Biosciences 610215, mouse | 1:1,000 |
| Tubulin | Li-Cor 926-42211, rabbit | 1:2,500 |
| GM130 | BD Biosciences 610822, mouse | 1:100 |
| CD63 | Gift from Andrew Peden (University of Sheffield, Sheffield, UK), mouse | 1:100 |
| EEA1 | Gift from Andrew Peden, rabbit | 1:100 |
| Syntaxin13 | Prekeris Lab (Prekeris et al., 1998), rabbit | 1:100 |
| Ubiquitin P4D1 | Santa Cruz sc-8017, mouse | 1:200 |
| GFP | Invitrogen A-11122, rabbit | 1:100 |
| GAPDH | CST 5174, rabbit | 1:1,000 |
| EGFR | CST 2232, rabbit | 1:1,000 |
| Myc 9E10 | Santa Cruz sc-40, mouse | 1:1,000 |
| HA | Santa Cruz sc-7392, mouse | 1:1,000 |
| FLAG-HRP conjugate | CST 86861, rabbit | 1:1,000 |
| AffiniPure goat anti-mouse IgG, light chain specific | Jackson ImmunoResearch 211-032-171 | 1:1,000 |
| IRDye 680RD anti-mouse secondary | Li-Cor 926-68072 | 1:2,500 |
| IRDye 800CW anti-rabbit secondary | Li-Cor 926-32213 | 1:2,500 |
| Alexa Fluor 568 phalloidin | Thermo Fisher Scientific a12380 | 1:40 |
| Alexa Fluor 488 anti-mouse secondary | Jackson ImmunoResearch 715-545-150 | 1:100 |
| Alexa Fluor 594 anti-mouse secondary | Jackson ImmunoResearch 715-585-150 | 1:100 |
| Alexa Fluor 594 anti-rabbit secondary | Jackson ImmunoResearch 711-585-152 | 1:100 |
| Hoechst 33342 | AnaSpec AS-83218 | 1:2,000 |
| jetPRIME transfection reagent | Polyplus 114-xx | N/A |
| DharmaFECT Duo transfection reagent | Horizon discovery T-2010-xx | N/A |
| Lipofectamine 2000 transfection reagent | Invitrogen 11668027 | N/A |
| tracrRNA | Horizon discovery U-002005-xx | N/A |
| Bafilomycin A1 | Selleckchem S1413 | 200 nM |
| MLN4924 | Selleckchem S7109 | 300 nM |
| Protein G Sepharose 4 Fast Flow | Cytiva 17-0618-01 | 50% |
| Bio-Rad Protein Assay Dye Reagent (Bradford method) | Bio-Rad 5000006 | 1:5 |
| Pierce Silver Stain Kit | Thermo Fisher Scientific 24612 | N/A |
| Intercept TBS Blocking Buffer | Li-Cor 927-60001 | 1:3 |
| CytoPhos Phosphate Assay | Cytoskeleton BK054 | N/A |
| Name . | Company, product number, host . | Dilution . |
|---|---|---|
| FLAG M2 | Sigma F3165, mouse | 1:1,000 |
| Rap2 | BD Biosciences 610215, mouse | 1:1,000 |
| Tubulin | Li-Cor 926-42211, rabbit | 1:2,500 |
| GM130 | BD Biosciences 610822, mouse | 1:100 |
| CD63 | Gift from Andrew Peden (University of Sheffield, Sheffield, UK), mouse | 1:100 |
| EEA1 | Gift from Andrew Peden, rabbit | 1:100 |
| Syntaxin13 | Prekeris Lab (Prekeris et al., 1998), rabbit | 1:100 |
| Ubiquitin P4D1 | Santa Cruz sc-8017, mouse | 1:200 |
| GFP | Invitrogen A-11122, rabbit | 1:100 |
| GAPDH | CST 5174, rabbit | 1:1,000 |
| EGFR | CST 2232, rabbit | 1:1,000 |
| Myc 9E10 | Santa Cruz sc-40, mouse | 1:1,000 |
| HA | Santa Cruz sc-7392, mouse | 1:1,000 |
| FLAG-HRP conjugate | CST 86861, rabbit | 1:1,000 |
| AffiniPure goat anti-mouse IgG, light chain specific | Jackson ImmunoResearch 211-032-171 | 1:1,000 |
| IRDye 680RD anti-mouse secondary | Li-Cor 926-68072 | 1:2,500 |
| IRDye 800CW anti-rabbit secondary | Li-Cor 926-32213 | 1:2,500 |
| Alexa Fluor 568 phalloidin | Thermo Fisher Scientific a12380 | 1:40 |
| Alexa Fluor 488 anti-mouse secondary | Jackson ImmunoResearch 715-545-150 | 1:100 |
| Alexa Fluor 594 anti-mouse secondary | Jackson ImmunoResearch 715-585-150 | 1:100 |
| Alexa Fluor 594 anti-rabbit secondary | Jackson ImmunoResearch 711-585-152 | 1:100 |
| Hoechst 33342 | AnaSpec AS-83218 | 1:2,000 |
| jetPRIME transfection reagent | Polyplus 114-xx | N/A |
| DharmaFECT Duo transfection reagent | Horizon discovery T-2010-xx | N/A |
| Lipofectamine 2000 transfection reagent | Invitrogen 11668027 | N/A |
| tracrRNA | Horizon discovery U-002005-xx | N/A |
| Bafilomycin A1 | Selleckchem S1413 | 200 nM |
| MLN4924 | Selleckchem S7109 | 300 nM |
| Protein G Sepharose 4 Fast Flow | Cytiva 17-0618-01 | 50% |
| Bio-Rad Protein Assay Dye Reagent (Bradford method) | Bio-Rad 5000006 | 1:5 |
| Pierce Silver Stain Kit | Thermo Fisher Scientific 24612 | N/A |
| Intercept TBS Blocking Buffer | Li-Cor 927-60001 | 1:3 |
| CytoPhos Phosphate Assay | Cytoskeleton BK054 | N/A |