Comparison of the direct myosin II effectors available in Drosophila
| Effector . | Genotype . | Dorsal closure phenotype . | Viability . |
|---|---|---|---|
| Rok-catT2A | sqh_Sqh::GFP, enGal4/+ ; Rok-catT2A/+ | Aberrant | Larval lethal |
| Rok-CAT3.1 | sqh_Sqh::GFP, enGal4/+ ; Rok-CAT3.1/+ | Wild type | Viable |
| N-Rok::vhhGFP4ZH-86Fb | sqh_Sqh::GFP, enGal4/+ ; N-Rok::vhhGFP4ZH-86Fb/+ | Aberrant/open | Embryonic lethal |
| N-Rok-HAZH-86Fb | sqh_Sqh::GFP, enGal4/+ ; N-Rok-HAZH-86Fb/+ | Wild type | Reduced viability |
| N-Rok::vhhGFP4Vi | sqh_Sqh::GFP, enGal4/+ ; N-Rok::vhhGFP4Vi/+ | Aberrant | Embryonic lethal |
| SqhE20E21 | enGal4/SqhE20E21 | Wild type | Viable |
| ctMLCK | sqh_Sqh::GFP, enGal4/+ ; ctMLCK/+ | Aberrant or normal but slower | Larval lethal |
| NSlmb::vhhGFP4 | flw::GFP, enGal4/+ ; NSlmb::vhhGFP4/+ | Wild type | Viable |
| Effector . | Genotype . | Dorsal closure phenotype . | Viability . |
|---|---|---|---|
| Rok-catT2A | sqh_Sqh::GFP, enGal4/+ ; Rok-catT2A/+ | Aberrant | Larval lethal |
| Rok-CAT3.1 | sqh_Sqh::GFP, enGal4/+ ; Rok-CAT3.1/+ | Wild type | Viable |
| N-Rok::vhhGFP4ZH-86Fb | sqh_Sqh::GFP, enGal4/+ ; N-Rok::vhhGFP4ZH-86Fb/+ | Aberrant/open | Embryonic lethal |
| N-Rok-HAZH-86Fb | sqh_Sqh::GFP, enGal4/+ ; N-Rok-HAZH-86Fb/+ | Wild type | Reduced viability |
| N-Rok::vhhGFP4Vi | sqh_Sqh::GFP, enGal4/+ ; N-Rok::vhhGFP4Vi/+ | Aberrant | Embryonic lethal |
| SqhE20E21 | enGal4/SqhE20E21 | Wild type | Viable |
| ctMLCK | sqh_Sqh::GFP, enGal4/+ ; ctMLCK/+ | Aberrant or normal but slower | Larval lethal |
| NSlmb::vhhGFP4 | flw::GFP, enGal4/+ ; NSlmb::vhhGFP4/+ | Wild type | Viable |
Dorsal closure in embryos and adult flies viability were used as a readout of myosin II activation in the en expression domain. Both the effectors and the full genotypes of the tested embryos are indicated to highlight all the components that the methods require. For every condition, more than 20 embryos were assessed. See text for details.