List of primers used in this study
| Name . | Nucleotide sequence . |
|---|---|
| Primers used to amplify the aphVIII gene insertion in ARL3 genomic DNA . | |
| gARL3-FOR | 5′-CCTCTAGAACATCACCACTATCACGC-3′ |
| gARL3-REV | 5′-CCGAATTCCCAAAGCTCCGAAGCCAC-3′ |
| Primers used to clone target genes | |
| gARL3-FOR1 | 5′-CCTCTAGAAGCTCCGACATGAGCGCC-3′ |
| gARL3-REV1 | 5′-CCGAATTCCTTGACCTGCTTCATCATC-3′ |
| gARL3ΔN15-FOR | 5′-CCGGATCCGTGACATGTGCTCGCAGC-3′ |
| gARL3ΔN15-REV | 5′-CCGGATCCCTGTGGCGAGCACACCAG-3′ |
| gIFT38-FOR | 5′-CCGGATCCATTTAGCCCCAAATTATG-3′ |
| gIFT38-REV | 5′-CCGAATTCGAAGTCATTGTCATCCTC-3′ |
| gIFT43-FOR | 5′-ACGCGGCCGCATGGTGTCCTACCTGGGC-3′ |
| gIFT43-REV | 5′-GTGAATTCCAGCTTGATGGGCATGG-3′ |
| gBBS8-FOR | 5′-GGACTAGTTGCAGCAGCAAGTAATGCAAC-3′ |
| gBBS8-REV | 5′-GGAATTCCAGCATAGTGAAATGGGCC-3′ |
| cARL3-FOR1 | 5′-GGCCATGGATGGGCCTCTTGTCGCTG-3′ |
| cARL3-REV1 | 5′-CGGCGGCCGCCTTGACCTGCTTCATC-3′ |
| cARL3ΔN15-FOR | 5′-CCCCATGGATGGCCCGCATCCTGGTC-3′ |
| cBBS1-FOR | 5′-CCGGATCCATGCTGCCATCAGTCAAG-3′ |
| cBBS1-REV | 5′-CCAAGCTTTTACTCCACCTCCTCCGG-3′ |
| cBBS2-FOR | 5′-CCGGATCCATGCTCGTGCCGGCCTTC-3′ |
| cBBS2-REV | 5′-CCAAGCTTTCACACCGGCCCGTCGCC-3′ |
| cBBS4-FOR | 5′-CAGCAAATGGGTCGGGATCCATGTCGTCATTAGCGCAG-3′ |
| cBBS4-REV | 5′-TCGACGGAGCTCGAATTCTTATCACATGCCCAGCAGCT-3′ |
| cBBS5-FOR | 5′-GACAGCAAATGGGTCGGGATCCATGGCTGACCTCTTTG-3′ |
| cBBS5-REV | 5′-CGACGGAGCTCGAATTCTTATCACAGCACGCTCCACAG-3′ |
| cBBS7-FOR | 5′-GGACAGCAAATGGGTCGGGATCCATGGAGCTGGAACTATTTC-3′ |
| cBBS7-REV | 5′-GTCGACGGAGCTCGAATTCTTACTACCGCTGCCCCAGCAG-3′ |
| cBBS8-FOR | 5′-GGACAGCAAATGGGTCGGGATCCATGCAGCAGCCACAGCAAG-3′ |
| cBBS8-REV | 5′-GTCGACGGAGCTCGAATTCTTATCACAGCATAGTGAAATG-3′ |
| Primers used to do site-directed mutagenesis | |
| ARL3Q70L-FOR | 5′-GGACATTGGCGGCCTGAAGTCCAT-3′ |
| ARL3Q70L-REV | 5′-AGGCCGCCAATGTCCCAAATTTTC-3′ |
| ARL3T30N-FOR | 5′-ATAACGCTGGTAAAAACACCATCCTG-3′ |
| ARL3T30N-REV | 5′-TTTTTACCAGCGTTATCCAGTCCCA-3′ |
| Name . | Nucleotide sequence . |
|---|---|
| Primers used to amplify the aphVIII gene insertion in ARL3 genomic DNA . | |
| gARL3-FOR | 5′-CCTCTAGAACATCACCACTATCACGC-3′ |
| gARL3-REV | 5′-CCGAATTCCCAAAGCTCCGAAGCCAC-3′ |
| Primers used to clone target genes | |
| gARL3-FOR1 | 5′-CCTCTAGAAGCTCCGACATGAGCGCC-3′ |
| gARL3-REV1 | 5′-CCGAATTCCTTGACCTGCTTCATCATC-3′ |
| gARL3ΔN15-FOR | 5′-CCGGATCCGTGACATGTGCTCGCAGC-3′ |
| gARL3ΔN15-REV | 5′-CCGGATCCCTGTGGCGAGCACACCAG-3′ |
| gIFT38-FOR | 5′-CCGGATCCATTTAGCCCCAAATTATG-3′ |
| gIFT38-REV | 5′-CCGAATTCGAAGTCATTGTCATCCTC-3′ |
| gIFT43-FOR | 5′-ACGCGGCCGCATGGTGTCCTACCTGGGC-3′ |
| gIFT43-REV | 5′-GTGAATTCCAGCTTGATGGGCATGG-3′ |
| gBBS8-FOR | 5′-GGACTAGTTGCAGCAGCAAGTAATGCAAC-3′ |
| gBBS8-REV | 5′-GGAATTCCAGCATAGTGAAATGGGCC-3′ |
| cARL3-FOR1 | 5′-GGCCATGGATGGGCCTCTTGTCGCTG-3′ |
| cARL3-REV1 | 5′-CGGCGGCCGCCTTGACCTGCTTCATC-3′ |
| cARL3ΔN15-FOR | 5′-CCCCATGGATGGCCCGCATCCTGGTC-3′ |
| cBBS1-FOR | 5′-CCGGATCCATGCTGCCATCAGTCAAG-3′ |
| cBBS1-REV | 5′-CCAAGCTTTTACTCCACCTCCTCCGG-3′ |
| cBBS2-FOR | 5′-CCGGATCCATGCTCGTGCCGGCCTTC-3′ |
| cBBS2-REV | 5′-CCAAGCTTTCACACCGGCCCGTCGCC-3′ |
| cBBS4-FOR | 5′-CAGCAAATGGGTCGGGATCCATGTCGTCATTAGCGCAG-3′ |
| cBBS4-REV | 5′-TCGACGGAGCTCGAATTCTTATCACATGCCCAGCAGCT-3′ |
| cBBS5-FOR | 5′-GACAGCAAATGGGTCGGGATCCATGGCTGACCTCTTTG-3′ |
| cBBS5-REV | 5′-CGACGGAGCTCGAATTCTTATCACAGCACGCTCCACAG-3′ |
| cBBS7-FOR | 5′-GGACAGCAAATGGGTCGGGATCCATGGAGCTGGAACTATTTC-3′ |
| cBBS7-REV | 5′-GTCGACGGAGCTCGAATTCTTACTACCGCTGCCCCAGCAG-3′ |
| cBBS8-FOR | 5′-GGACAGCAAATGGGTCGGGATCCATGCAGCAGCCACAGCAAG-3′ |
| cBBS8-REV | 5′-GTCGACGGAGCTCGAATTCTTATCACAGCATAGTGAAATG-3′ |
| Primers used to do site-directed mutagenesis | |
| ARL3Q70L-FOR | 5′-GGACATTGGCGGCCTGAAGTCCAT-3′ |
| ARL3Q70L-REV | 5′-AGGCCGCCAATGTCCCAAATTTTC-3′ |
| ARL3T30N-FOR | 5′-ATAACGCTGGTAAAAACACCATCCTG-3′ |
| ARL3T30N-REV | 5′-TTTTTACCAGCGTTATCCAGTCCCA-3′ |