List of primary antibodies used in this study
| Name . | IB . | IS . | Host . | Reference or source . |
|---|---|---|---|---|
| Anti-ARL3 | 1:250 | N/A | Rb | This study |
| Anti-CEP290 | 1:250 | 1:50 | Rb | This study |
| Anti-IFT22 | 1:1,000 | N/A | Rb | (Xue et al., 2020) |
| Anti-IFT38 | 1:1,000 | N/A | Rb | (Sun et al., 2021) |
| Anti-IFT46 | N/A | 1:100 | Rb | (Dong et al., 2017a) |
| Anti-IFT57 | 1:500 | N/A | Rb | (Dong et al., 2017a) |
| Anti-IFT43 | 1:500 | N/A | Rb | (Zhu et al., 2017) |
| Anti-IFT70 | 1:1,000 | N/A | Rb | (Dong et al., 2017a) |
| Anti-IFT81 | N/A | 1:200 | Mo | (Fan et al., 2010) |
| Anti-IFT139 | 1:1,000 | N/A | Rb | (Dong et al., 2017a) |
| Anti-BBS1 | 1:1,000 | N/A | Rb | (Xue et al., 2020) |
| Anti-BBS4 | 1:500 | N/A | Rb | (Liu et al., 2021) |
| Anti-BBS5 | 1:1,000 | N/A | Rb | (Xue et al., 2020) |
| Anti-BBS7 | 1:500 | N/A | Rb | (Liu et al., 2021) |
| Anti-BBS8 | 1:500 | 1:50 | Rb | (Sun et al., 2021) |
| Anti-PLD | 1:1,000 | N/A | Rb | (Liu et al., 2021) |
| Anti-α-tubulin (B512) | 1:10,000 | N/A | Mo | Sigma-Aldrich (T5168) |
| Anti-acetylated-tubulin (6-11B-1) | 1:10,000 | N/A | Mo | Sigma-Aldrich (T7451) |
| Anti-YFP (7.1 and 13.1) | 1:1,000 | 1:50 | Mo | Roche (11814460001) |
| Anti-HA (3F10) | 1:1,000 | 1:50 | Rt | Roche (11867423001) |
| HRP-conjugated goat anti-rabbit IgG | 1:10,000 | N/A | Gt | The Jackson Lab. (111035003) |
| HRP-conjugated goat anti-mouse IgG | 1:10,000 | N/A | Gt | The Jackson Lab. (115035003) |
| HRP-conjugated goat anti-rat IgG | 1:10,000 | N/A | Gt | The Jackson Lab. (112035003) |
| Alexa-Fluor 594-conjugated goat anti-rabbit IgG | N/A | 1:400 | Gt | Molecular probes (A11012) |
| Alexa-Flour 488-conjugated goat anti-mouse IgG | N/A | 1:400 | Gt | Molecular probes (A10680) |
| Alexa-Flour 488-conjugated goat anti-rat IgG | N/A | 1:400 | Gt | Molecular probes (A11006) |
| Name . | IB . | IS . | Host . | Reference or source . |
|---|---|---|---|---|
| Anti-ARL3 | 1:250 | N/A | Rb | This study |
| Anti-CEP290 | 1:250 | 1:50 | Rb | This study |
| Anti-IFT22 | 1:1,000 | N/A | Rb | (Xue et al., 2020) |
| Anti-IFT38 | 1:1,000 | N/A | Rb | (Sun et al., 2021) |
| Anti-IFT46 | N/A | 1:100 | Rb | (Dong et al., 2017a) |
| Anti-IFT57 | 1:500 | N/A | Rb | (Dong et al., 2017a) |
| Anti-IFT43 | 1:500 | N/A | Rb | (Zhu et al., 2017) |
| Anti-IFT70 | 1:1,000 | N/A | Rb | (Dong et al., 2017a) |
| Anti-IFT81 | N/A | 1:200 | Mo | (Fan et al., 2010) |
| Anti-IFT139 | 1:1,000 | N/A | Rb | (Dong et al., 2017a) |
| Anti-BBS1 | 1:1,000 | N/A | Rb | (Xue et al., 2020) |
| Anti-BBS4 | 1:500 | N/A | Rb | (Liu et al., 2021) |
| Anti-BBS5 | 1:1,000 | N/A | Rb | (Xue et al., 2020) |
| Anti-BBS7 | 1:500 | N/A | Rb | (Liu et al., 2021) |
| Anti-BBS8 | 1:500 | 1:50 | Rb | (Sun et al., 2021) |
| Anti-PLD | 1:1,000 | N/A | Rb | (Liu et al., 2021) |
| Anti-α-tubulin (B512) | 1:10,000 | N/A | Mo | Sigma-Aldrich (T5168) |
| Anti-acetylated-tubulin (6-11B-1) | 1:10,000 | N/A | Mo | Sigma-Aldrich (T7451) |
| Anti-YFP (7.1 and 13.1) | 1:1,000 | 1:50 | Mo | Roche (11814460001) |
| Anti-HA (3F10) | 1:1,000 | 1:50 | Rt | Roche (11867423001) |
| HRP-conjugated goat anti-rabbit IgG | 1:10,000 | N/A | Gt | The Jackson Lab. (111035003) |
| HRP-conjugated goat anti-mouse IgG | 1:10,000 | N/A | Gt | The Jackson Lab. (115035003) |
| HRP-conjugated goat anti-rat IgG | 1:10,000 | N/A | Gt | The Jackson Lab. (112035003) |
| Alexa-Fluor 594-conjugated goat anti-rabbit IgG | N/A | 1:400 | Gt | Molecular probes (A11012) |
| Alexa-Flour 488-conjugated goat anti-mouse IgG | N/A | 1:400 | Gt | Molecular probes (A10680) |
| Alexa-Flour 488-conjugated goat anti-rat IgG | N/A | 1:400 | Gt | Molecular probes (A11006) |
Rb, rabbit; Mo, mouse; Rt, rat; Gt, goat; IB, immunoblotting; IS, immunostaining.