| Antibody . | WB concentration . | IF concentration . | Source . |
|---|---|---|---|
| α-Tubulin mouse monoclonal | — | 1:1,000 | Sigma-Aldrich; DM1a |
| Asl (N-terminal) guinea pig polyclonal | 1:1,000 | 1:1,000 | Gift from Jordan Raff |
| Cnn (N-terminal) rabbit monoclonal | 1:1,000 | 1:1,000 | Gift from Jordan Raff |
| Cnn (C-terminal) sheep polyclonal | 1:1,000 | — | Gift from Jordan Raff |
| Cnn-TN rabbit polyclonal | 1:500 | — | This study |
| γ-Tubulin mouse monoclonal | 1:500 | 1:500 | Sigma-Aldrich; GTU-88 |
| γ-Tubulin rabbit polyclonal | — | 1:500 | Sigma-Aldrich; T5192 |
| GFP mouse monoclonal | 1:250 or 1:500 | 1:250 or 1:500 | Roche, 11814460001 |
| Grip71 rabbit polyclonal | 1:100 | 1:100 | CRB (crb2005268) |
| MBP rabbit polyclonal | 1:3,000 | — | Gift from Jordan Raff |
| Phospho-histone H3 rabbit polyclonal | — | 1:500 | Abcam; AB5176 |
| Staufen mouse monoclonal | — | 1:100 | Santa Cruz Biotechnology; dN-16 |
| Gurken mouse monoclonal | — | 1:200 | DSHB; 1D12 |
| Lamin Dm0 | — | 1:30 | DSHB; 84.12 |
| Antibody . | WB concentration . | IF concentration . | Source . |
|---|---|---|---|
| α-Tubulin mouse monoclonal | — | 1:1,000 | Sigma-Aldrich; DM1a |
| Asl (N-terminal) guinea pig polyclonal | 1:1,000 | 1:1,000 | Gift from Jordan Raff |
| Cnn (N-terminal) rabbit monoclonal | 1:1,000 | 1:1,000 | Gift from Jordan Raff |
| Cnn (C-terminal) sheep polyclonal | 1:1,000 | — | Gift from Jordan Raff |
| Cnn-TN rabbit polyclonal | 1:500 | — | This study |
| γ-Tubulin mouse monoclonal | 1:500 | 1:500 | Sigma-Aldrich; GTU-88 |
| γ-Tubulin rabbit polyclonal | — | 1:500 | Sigma-Aldrich; T5192 |
| GFP mouse monoclonal | 1:250 or 1:500 | 1:250 or 1:500 | Roche, 11814460001 |
| Grip71 rabbit polyclonal | 1:100 | 1:100 | CRB (crb2005268) |
| MBP rabbit polyclonal | 1:3,000 | — | Gift from Jordan Raff |
| Phospho-histone H3 rabbit polyclonal | — | 1:500 | Abcam; AB5176 |
| Staufen mouse monoclonal | — | 1:100 | Santa Cruz Biotechnology; dN-16 |
| Gurken mouse monoclonal | — | 1:200 | DSHB; 1D12 |
| Lamin Dm0 | — | 1:30 | DSHB; 84.12 |
IF, immunofluorescence; WB, Western blotting.