Table 1.
Primers used in the study
Forward primerReverse primer
Cnn-C-N fragment 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GGA​CCA​GTC​TAA​ACA​GGT​TTT​GC-3′ 5′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​CTA​TAG​GCG​CTC​GGC​CAA​C-3′ 
Cnn-T-N fragment 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GAA​TAG​TAA​TCG​AAC​GTC​GTC​TTC​G-3′ 5′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​CTA​TAG​GCG​CTC​GGC​CAA​C-3′ 
Cnn-C-NP1 insert 5′-GCG​GGA​CTA​TTG​CGG​CGA​CGG​CAA​TGG​TAC​CTG​TGC​AGA​CGA​CTT​GAA​GGA​AAT​CGA​GTT​AAT​TGA​GGA​GGT​GG-3′ 5′-GCA​GGA​CCC​TTC​TGT​CGA​TTT​CGG​CGG​CGC​CAT​TCT​CCT​CCA​GGA​AGT​CCT​CCA​CCT​CCT​CAA​TTA​ACT​CGA​TTT​CC-3′ 
Cnn-C-NP2 insert 5′-CCT​GCG​CAA​ACT​AGC​CGA​GGC​ACT​GGA​CAA​AGA​CAT​AGA​CGA​CGA​GGA​CCC​GGG​AGC​CCT​GCA​AGA​TGT​CG-3′ 5′-CGC​CTG​GAG​GTC​GTG​GAA​CGT​CAA​AGT​CGG​CAT​AGT​CGT​TCT​CCA​TCT​CGA​CAT​CTT​GCA​GGG​CTC​CCG​GGT​CC-3′ 
Cnn-C-NP3 insert 5′-GGG​TCA​GCC​GGG​TGC​CCG​GGC​AGA​CGA​CGA​CGA​GGA​AGA​CTT​AGA​CAA​ACA​GCT​CAT​CGA​TGC​CAA​GAT​CGA​AAT​CGC-3′ 5′-CCT​TGA​GCA​GCT​CCA​TCT​TTA​CAT​CGA​CCT​CTT​TTC​TCA​ACT​CCG​CGA​TTT​CGA​TCT​TGG​CAT​CGA​TGA​GC-3′ 
Cnn-C-NP1a insert 5′-GCG​GGA​CTA​TTG​CGG​CGA​CGG​CAA​TGG​TAC​CTG​TGC​AGA​CGA​CTT​GAA​GGA​AAT​CGA​GTT​AAT​TGA​GAC​CGT​GA-3′ 5′-GGA​CCC​TTC​TGT​CGA​TTT​CGG​CGG​CGC​CAT​TCT​CCT​CCA​GGA​AAC​TGG​TCA​CGG​TCT​CAA​TTA​ACT​CGA​TTT​CCT​TC-3′ 
Cnn-C-NP1b insert 5′-GCG​GGA​CTA​TTG​CGG​CGA​CGG​CAA​TGG​TAC​CTG​TGC​ATC​GTC​CTT​GAA​GGA​AAT​CAC​CTT​AAT​TG-3′ 5′-GGA​CCC​TTC​TGT​CGA​TTT​CGG​CGG​CGC​CAT​TCT​CCT​CCA​GGA​AGT​CCT​CCA​CCT​CCT​CAA​TTA​AGG​TGA​TTT​CCT​TC-3′ 
Cnn-C-NT27 insert 5′-GCG​GGA​CTA​TTG​CGG​CGA​CGG​CAA​TGG​TAC​CTG​TGC​ATC​GTC​CTT​GAA​GGA​AAT​CGA​GTT​AAT​TGA​GAC​CGT​GA-3′ 5′-GGA​CCC​TTC​TGT​CGA​TTT​CGG​CGG​CGC​CAT​TCT​CCT​CCA​GGA​AAC​TGG​TCA​CGG​TCT​CAA​TTA​ACT​CGA​TTT​CCT​TC-3′ 
Cnn-C-NΔ1-77 5′-GCC​AAC​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GGC​CAG​TTT​TGA​CGT​TCC-3′ 5′-GGA​ACG​TCA​AAA​CTG​GCC​ATT​AAG​CCT​GCT​TTT​TTG​TAC​AAA​GTT​GGC-3′ 
CDK5RAP2 aa 1–210 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GAT​GGA​CTT​GGT​GTT​GGA​AGA​GG 5′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​TCA​CAA​GTC​CCC​CTC​GTG​CAT​CTT​C-3′ 
CDK5RAP2 aa 51–100 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GAC​AGT​GTC​TCC​CAC​CAG​AGC​ACG-3′ 5′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​TCA​GTA​GAT​ATG​TTC​AGT​GGG-3′ 
CDK5RAP2 aa 51–210 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GAC​AGT​GTC​TCC​CAC​CAG​AGC​ACG-3′ 5′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​TCA​CAA​GTC​CCC​CTC​GTG​CAT​CTT​C-3′ 
CDK5RAP2 aa 1–100 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GAT​GGA​CTT​GGT​GTT​GGA​AGA​GG-3′ 5′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​TCA​GTA​GAT​ATG​TTC​AGT​GGG-3′ 
Forward primerReverse primer
Cnn-C-N fragment 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GGA​CCA​GTC​TAA​ACA​GGT​TTT​GC-3′ 5′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​CTA​TAG​GCG​CTC​GGC​CAA​C-3′ 
Cnn-T-N fragment 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GAA​TAG​TAA​TCG​AAC​GTC​GTC​TTC​G-3′ 5′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​CTA​TAG​GCG​CTC​GGC​CAA​C-3′ 
Cnn-C-NP1 insert 5′-GCG​GGA​CTA​TTG​CGG​CGA​CGG​CAA​TGG​TAC​CTG​TGC​AGA​CGA​CTT​GAA​GGA​AAT​CGA​GTT​AAT​TGA​GGA​GGT​GG-3′ 5′-GCA​GGA​CCC​TTC​TGT​CGA​TTT​CGG​CGG​CGC​CAT​TCT​CCT​CCA​GGA​AGT​CCT​CCA​CCT​CCT​CAA​TTA​ACT​CGA​TTT​CC-3′ 
Cnn-C-NP2 insert 5′-CCT​GCG​CAA​ACT​AGC​CGA​GGC​ACT​GGA​CAA​AGA​CAT​AGA​CGA​CGA​GGA​CCC​GGG​AGC​CCT​GCA​AGA​TGT​CG-3′ 5′-CGC​CTG​GAG​GTC​GTG​GAA​CGT​CAA​AGT​CGG​CAT​AGT​CGT​TCT​CCA​TCT​CGA​CAT​CTT​GCA​GGG​CTC​CCG​GGT​CC-3′ 
Cnn-C-NP3 insert 5′-GGG​TCA​GCC​GGG​TGC​CCG​GGC​AGA​CGA​CGA​CGA​GGA​AGA​CTT​AGA​CAA​ACA​GCT​CAT​CGA​TGC​CAA​GAT​CGA​AAT​CGC-3′ 5′-CCT​TGA​GCA​GCT​CCA​TCT​TTA​CAT​CGA​CCT​CTT​TTC​TCA​ACT​CCG​CGA​TTT​CGA​TCT​TGG​CAT​CGA​TGA​GC-3′ 
Cnn-C-NP1a insert 5′-GCG​GGA​CTA​TTG​CGG​CGA​CGG​CAA​TGG​TAC​CTG​TGC​AGA​CGA​CTT​GAA​GGA​AAT​CGA​GTT​AAT​TGA​GAC​CGT​GA-3′ 5′-GGA​CCC​TTC​TGT​CGA​TTT​CGG​CGG​CGC​CAT​TCT​CCT​CCA​GGA​AAC​TGG​TCA​CGG​TCT​CAA​TTA​ACT​CGA​TTT​CCT​TC-3′ 
Cnn-C-NP1b insert 5′-GCG​GGA​CTA​TTG​CGG​CGA​CGG​CAA​TGG​TAC​CTG​TGC​ATC​GTC​CTT​GAA​GGA​AAT​CAC​CTT​AAT​TG-3′ 5′-GGA​CCC​TTC​TGT​CGA​TTT​CGG​CGG​CGC​CAT​TCT​CCT​CCA​GGA​AGT​CCT​CCA​CCT​CCT​CAA​TTA​AGG​TGA​TTT​CCT​TC-3′ 
Cnn-C-NT27 insert 5′-GCG​GGA​CTA​TTG​CGG​CGA​CGG​CAA​TGG​TAC​CTG​TGC​ATC​GTC​CTT​GAA​GGA​AAT​CGA​GTT​AAT​TGA​GAC​CGT​GA-3′ 5′-GGA​CCC​TTC​TGT​CGA​TTT​CGG​CGG​CGC​CAT​TCT​CCT​CCA​GGA​AAC​TGG​TCA​CGG​TCT​CAA​TTA​ACT​CGA​TTT​CCT​TC-3′ 
Cnn-C-NΔ1-77 5′-GCC​AAC​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GGC​CAG​TTT​TGA​CGT​TCC-3′ 5′-GGA​ACG​TCA​AAA​CTG​GCC​ATT​AAG​CCT​GCT​TTT​TTG​TAC​AAA​GTT​GGC-3′ 
CDK5RAP2 aa 1–210 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GAT​GGA​CTT​GGT​GTT​GGA​AGA​GG 5′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​TCA​CAA​GTC​CCC​CTC​GTG​CAT​CTT​C-3′ 
CDK5RAP2 aa 51–100 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GAC​AGT​GTC​TCC​CAC​CAG​AGC​ACG-3′ 5′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​TCA​GTA​GAT​ATG​TTC​AGT​GGG-3′ 
CDK5RAP2 aa 51–210 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GAC​AGT​GTC​TCC​CAC​CAG​AGC​ACG-3′ 5′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​TCA​CAA​GTC​CCC​CTC​GTG​CAT​CTT​C-3′ 
CDK5RAP2 aa 1–100 5′-GGG​GAC​AAG​TTT​GTA​CAA​AAA​AGC​AGG​CTT​AAT​GAT​GGA​CTT​GGT​GTT​GGA​AGA​GG-3′ 5′-GGG​GAC​CAC​TTT​GTA​CAA​GAA​AGC​TGG​GTT​TCA​GTA​GAT​ATG​TTC​AGT​GGG-3′ 
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