| Vector . | Description . | Source . |
|---|---|---|
| pDONR221 | Gateway donor vector | Thermo Fisher Scientific |
| pENTR1A | Gateway entry vector | Thermo Fisher Scientific |
| pDestMyc | Mammalian N-terminal Myc-tag fusion expression vector with CMV and T7 promoters | (Lamark et al., 2003) |
| pDest-3xFLAG | Mammalian N-terminal 3xFlag-tag fusion expression vector with CMV promoter | (Jain et al., 2010) |
| pDest15 | Bacterial GST fusion expression vector with a T7 promoter | Thermo Fisher Scientific |
| pENTR223-SAMM50 | Human SAMM50 in Gateway entry vector | This study |
| pDestMyc-SAMM50 | Human SAMM50 with N-terminal Myc tag | This study |
| pDest15 SAMM50 | Human SAMM50 with N-terminal GST fusion tag | This study |
| pDest15 p62 | Human p62 with N-terminal GST fusion tag | (Jain et al., 2010) |
| pDest 3xFLAG | Mammalian triple FLAG tag fusion expression vector with CMV promoter | (Jain et al., 2010) |
| pDest 3xFLAG p62 | Human p62 with N-terminal 3xFLAG tag | (Jain et al., 2010) |
| pDest 3xFLAG LC3B | Human LC3B with N-terminal 3xFLAG tag | This study |
| pDest 3xFLAG GABARAP | Human GABARAP with N-terminal 3xFLAG tag | This study |
| pDest15 p62 ΔPB1 | Human p62 with PB1 domain deletion and N-terminal GST fusion tag | This study |
| pDest15 p62 Δ123–170 | Human p62 with 123–170 deletion and N-terminal GST fusion tag | This study |
| pDest15 p62 Δ170–256 | Human p62 with 170–256 deletion and N-terminal GST fusion tag | This study |
| pDest15 p62 Δ256–370 | Human p62 with 256–370 deletion and N-terminal GST fusion tag | This study |
| pDest15 p62 Δ371–385 | Human p62 with 371–385 deletion and N-terminal GST fusion tag | This study |
| pDest15 p62 ΔUBA | Human p62 with UBA domain deletion and N-terminal GST fusion tag | This study |
| pDONR221 MIC60 | Human MIC60 in Gateway donor vector | Harvard |
| pDest15 MIC60 | Human MIC60 with N-terminal GST fusion tag | This study |
| pENTR223 MIC19 | Human MIC19 in Gateway entry vector | Harvard |
| pDest15 MIC19 | Human MIC19 with N-terminal GST fusion tag | This study |
| pENTR VDAC1 | Human VDAC1 in Gateway entry vector | This study |
| pDest15 VDAC1 | Human VDAC1 with N-terminal GST fusion tag | This study |
| pENTR MTX1 | Human MTX1 in Gateway entry vector | This study |
| pDest15 MTX1 | Human MTX1 with N-terminal GST fusion tag | This study |
| pDestMyc-MTX1 | Human MTX1 with N-terminal Myc tag | This study |
| pENTR MTX2 | Human MTX2 in Gateway entry vector | This study |
| pDestMyc-MTX2 | Human MTX2 with N-terminal Myc tag | This study |
| pDest15 MTX2 | Human MIC60 with N-terminal GST fusion tag | This study |
| pDONR221 SAMM50 Δ1–40 | Human SAMM50 with 1–40 deletion in Gateway donor vector | This study |
| pDONR221 SAMM50 Δ1–70 | Human SAMM50 with 1–70 deletion in Gateway donor vector | This study |
| pDONR221 SAMM50 Δ1–100 | Human SAMM50 with 1–100 deletion in Gateway donor vector | This study |
| pDONR221 SAMM50 Δ1–125 | Human SAMM50 with 1–125 deletion in Gateway donor vector | This study |
| pDestMyc SAMM50 Δ1–40 | Human SAMM50 with 1–40 deletion and N-terminal Myc tag | This study |
| pDestMyc SAMM50 Δ1–70 | Human SAMM50 with 1–70 deletion and N-terminal Myc tag | This study |
| pDestMyc SAMM50 Δ1–100 | Human SAMM50 with 1–100 deletion and N-terminal Myc tag | This study |
| pDestMyc SAMM50 Δ1–125 | Human SAMM50 with 1–125 deletion and N-terminal Myc tag | This study |
| pMXS-puro | Retroviral vector with Moloney murine leukemia virus promoter | CellBiolabs RTV-012 |
| pMRXIP | Retroviral vector and MMLV promoter (backbone is pMRXIP-GFP-STX17; Addgene, 45909) | This study |
| pMXs puro-mCherry PARKIN | Retroviral vector with Human PARKIN and N-terminal mCherry fusion tag | This study |
| pMXs puro-Myc-SAMM50 | Retroviral vector with Human SAMM50 and N-terminal Myc tag | This study |
| pMXs puro-Myc SAMM50 Δ1–40 | Retroviral vector with Human SAMM50 Δ1–40 and N-terminal Myc tag | This study |
| pMXs puro-Myc SAMM50 Δ1–70 | Retroviral vector with Human SAMM50 Δ1–70 and N-terminal Myc tag | This study |
| pMXs puro-Myc SAMM50 Δ1–100 | Retroviral vector with Human SAMM50 Δ1–100 and N-terminal Myc tag | This study |
| pMXs puro-Myc SAMM50 Δ1–125 | Retroviral vector with Human SAMM50 Δ1–125 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 | Retroviral vector with Human SAMM50 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–40 | Retroviral vector with Human SAMM50 Δ1–40 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–70 | Retroviral vector with Human SAMM50 Δ1–70 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–100 | Retroviral vector with Human SAMM50 Δ1–100 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–125 | Retroviral vector with Human SAMM50 Δ1–125 and N-terminal Myc tag | This study |
| pMRXIP GFP-SAMM50 | Retroviral vector with Human SAMM50 and N-terminal GFP fusion tag | This study |
| pMRXIP GFP-SAMM50 Δ1–125 | Retroviral vector with Human SAMM50 Δ1–125 and N-terminal GFP fusion tag | This study |
| pDest15 LC3A | Human LC3A with N-terminal GST fusion tag | (Pankiv et al., 2007) |
| pDest15 LC3B | Human LC3B with N-terminal GST fusion tag | Pankiv et al., 2007 |
| pDest15 LC3C | Human LC3C with N-terminal GST fusion tag | (Pankiv et al., 2007) |
| pDest15 GABARAP | Human GABARAP with N-terminal GST fusion tag | (Pankiv et al., 2007) |
| pDest15 GABARAPL1 | Human GABARAPL1 with N-terminal GST fusion tag | (Pankiv et al., 2007) |
| pDest15 GABARAPL2 | HumanGABARAPL2 with N-terminal GST fusion tag | (Pankiv et al., 2007) |
| pDest15 GABARAP Y49A | Human GABARAP Y49A with N-terminal GST fusion tag | This study |
| pDONR221 SAMM50 F28A/V31A | Human SAMM50 with F28A/V31A point mutations in Gateway donor vector | This study |
| pDONR221 SAMM50 Δ28–31 | Human SAMM50 with 28–31 deletion in Gateway donor vector | This study |
| pDONR221 SAMM50 Δ24–35 | Human SAMM50 with 24–35 deletion in Gateway donor vector | This study |
| pDestMyc SAMM50 F28A/V31A | Human SAMM50 with F28A/V31A point mutations and N-terminal Myc tag | This study |
| pDestMyc SAMM50 Δ28–31 | Human SAMM50 with 28–31 deletion and N-terminal Myc tag | This study |
| pDestMyc SAMM50 Δ24–35 | Human SAMM50 with 24–35 deletion and N-terminal Myc tag | This study |
| pMXs-puro mCherry-EGFP OMP25TM | Retroviral vector with tandem tag mCherry-EGFP OMP25TM mitophagy reporter | This study |
| pMRXIP Myc-LC3A | Retroviral vector with Human LC3A and N-terminal Myc tag | This study |
| pMRXIP Myc-LC3B | Retroviral vector with Human LC3B and N-terminal Myc tag | This study |
| pMRXIP Myc-LC3C | Retroviral vector with Human LC3C and N-terminal Myc tag | This study |
| pMRXIP Myc-GABARAP | Retroviral vector with Human GABARAP and N-terminal Myc tag | This study |
| pMRXIP Myc-GABARAPL1 | Retroviral vector with Human GABARAPL1 and N-terminal Myc tag | This study |
| pMRXIP Myc-GABARAPL2 | Retroviral vector with Human GABARAPL2and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 | Retroviral vector with Human SAMM50 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–40 | Retroviral vector with Human SAMM50 Δ1–40 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–70 | Retroviral vector with Human SAMM50 Δ1–70 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–100 | Retroviral vector with Human SAMM50 Δ1–100 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–125 | Retroviral vector with Human SAMM50 Δ1–125 and N-terminal Myc tag | This study |
| pMRXIP COX8-EGFP-mCherry | Retroviral vector with tandem tag COX8-EGFP-mCherry mitophagy reporter | This study |
| pCDNA5/FRT-mNG1-10 | Flip-In vector with modified mNeonGreen β-sheet 1–10 (mNG1-10) | This study |
| pMRXIP-mNG11-SAMM50 | Retroviral vector with the Human SAMM50 and N-terminal mNG11 tag | This study |
| pMRXIP-mNG11-SAMM50 | Retroviral vector with the Human p62 and N-terminal mNG11 tag | This study |
| pCDNA5/FRT-MIC19-EGFP | Flip-In vector with Human MIC19 and C-terminal EGFP tag | This study |
| pMRXIP-MIC19-mCherry | Retroviral vector with Human MIC19 and C-terminal mCherry tag | This study |
| pCDNA5/FRT-LAMP1-mCherry | Flip-In vector with Human LAMP1 and C-terminal mCherry tag | This study |
| pMRXIP-LAMP1-EGFP | Retroviral vector with Human LAMP1 and C-terminal EGFP tag | This study |
| pMRXIP-EGFP-p62 | Retroviral vector with Human p62 and N-terminal EGFP tag | This study |
| pMRXIP-EGFP-LC3A | Retroviral vector with Human LC3A and N-terminal EGFP tag | This study |
| pMRXIP-EGFP-GABARAP | Retroviral vector with Human GABARAP and N-terminal EGFP tag | This study |
| pCDH-EF1α-GW-IRES-puromycin | Lentiviral vector with eukaryotic elongation factor 1 alpha (EF1α) promoter | System Biosciences |
| pCDH-EF1α-GW-IRES-puromycin-mCherry-p62 | Lentiviral vector with p62 and N-terminal mCherry tag | This study |
| pCDH-EF1α-GW-IRES-puromycin-Myc-p62 | Lentiviral vector with p62 and N-terminal Myc tag | This study |
| pCDH-EF1α-GW-IRES-puromycin-Myc-p62 Δ170–256 | Lentiviral vector with p62 Δ170-256 and N-terminal mCherry tag | This study |
| pCDH-EF1α-GW-IRES-puromycin-mCherry-ATG13 | Lentiviral vector with ATG13 and N-terminal mCherry tag | This study |
| pCDH-EF1α-GW-IRES-puromycin-mCherry-GABARAP | Lentiviral vector with GABARAP and N-terminal mCherry tag | This study |
| Vector . | Description . | Source . |
|---|---|---|
| pDONR221 | Gateway donor vector | Thermo Fisher Scientific |
| pENTR1A | Gateway entry vector | Thermo Fisher Scientific |
| pDestMyc | Mammalian N-terminal Myc-tag fusion expression vector with CMV and T7 promoters | (Lamark et al., 2003) |
| pDest-3xFLAG | Mammalian N-terminal 3xFlag-tag fusion expression vector with CMV promoter | (Jain et al., 2010) |
| pDest15 | Bacterial GST fusion expression vector with a T7 promoter | Thermo Fisher Scientific |
| pENTR223-SAMM50 | Human SAMM50 in Gateway entry vector | This study |
| pDestMyc-SAMM50 | Human SAMM50 with N-terminal Myc tag | This study |
| pDest15 SAMM50 | Human SAMM50 with N-terminal GST fusion tag | This study |
| pDest15 p62 | Human p62 with N-terminal GST fusion tag | (Jain et al., 2010) |
| pDest 3xFLAG | Mammalian triple FLAG tag fusion expression vector with CMV promoter | (Jain et al., 2010) |
| pDest 3xFLAG p62 | Human p62 with N-terminal 3xFLAG tag | (Jain et al., 2010) |
| pDest 3xFLAG LC3B | Human LC3B with N-terminal 3xFLAG tag | This study |
| pDest 3xFLAG GABARAP | Human GABARAP with N-terminal 3xFLAG tag | This study |
| pDest15 p62 ΔPB1 | Human p62 with PB1 domain deletion and N-terminal GST fusion tag | This study |
| pDest15 p62 Δ123–170 | Human p62 with 123–170 deletion and N-terminal GST fusion tag | This study |
| pDest15 p62 Δ170–256 | Human p62 with 170–256 deletion and N-terminal GST fusion tag | This study |
| pDest15 p62 Δ256–370 | Human p62 with 256–370 deletion and N-terminal GST fusion tag | This study |
| pDest15 p62 Δ371–385 | Human p62 with 371–385 deletion and N-terminal GST fusion tag | This study |
| pDest15 p62 ΔUBA | Human p62 with UBA domain deletion and N-terminal GST fusion tag | This study |
| pDONR221 MIC60 | Human MIC60 in Gateway donor vector | Harvard |
| pDest15 MIC60 | Human MIC60 with N-terminal GST fusion tag | This study |
| pENTR223 MIC19 | Human MIC19 in Gateway entry vector | Harvard |
| pDest15 MIC19 | Human MIC19 with N-terminal GST fusion tag | This study |
| pENTR VDAC1 | Human VDAC1 in Gateway entry vector | This study |
| pDest15 VDAC1 | Human VDAC1 with N-terminal GST fusion tag | This study |
| pENTR MTX1 | Human MTX1 in Gateway entry vector | This study |
| pDest15 MTX1 | Human MTX1 with N-terminal GST fusion tag | This study |
| pDestMyc-MTX1 | Human MTX1 with N-terminal Myc tag | This study |
| pENTR MTX2 | Human MTX2 in Gateway entry vector | This study |
| pDestMyc-MTX2 | Human MTX2 with N-terminal Myc tag | This study |
| pDest15 MTX2 | Human MIC60 with N-terminal GST fusion tag | This study |
| pDONR221 SAMM50 Δ1–40 | Human SAMM50 with 1–40 deletion in Gateway donor vector | This study |
| pDONR221 SAMM50 Δ1–70 | Human SAMM50 with 1–70 deletion in Gateway donor vector | This study |
| pDONR221 SAMM50 Δ1–100 | Human SAMM50 with 1–100 deletion in Gateway donor vector | This study |
| pDONR221 SAMM50 Δ1–125 | Human SAMM50 with 1–125 deletion in Gateway donor vector | This study |
| pDestMyc SAMM50 Δ1–40 | Human SAMM50 with 1–40 deletion and N-terminal Myc tag | This study |
| pDestMyc SAMM50 Δ1–70 | Human SAMM50 with 1–70 deletion and N-terminal Myc tag | This study |
| pDestMyc SAMM50 Δ1–100 | Human SAMM50 with 1–100 deletion and N-terminal Myc tag | This study |
| pDestMyc SAMM50 Δ1–125 | Human SAMM50 with 1–125 deletion and N-terminal Myc tag | This study |
| pMXS-puro | Retroviral vector with Moloney murine leukemia virus promoter | CellBiolabs RTV-012 |
| pMRXIP | Retroviral vector and MMLV promoter (backbone is pMRXIP-GFP-STX17; Addgene, 45909) | This study |
| pMXs puro-mCherry PARKIN | Retroviral vector with Human PARKIN and N-terminal mCherry fusion tag | This study |
| pMXs puro-Myc-SAMM50 | Retroviral vector with Human SAMM50 and N-terminal Myc tag | This study |
| pMXs puro-Myc SAMM50 Δ1–40 | Retroviral vector with Human SAMM50 Δ1–40 and N-terminal Myc tag | This study |
| pMXs puro-Myc SAMM50 Δ1–70 | Retroviral vector with Human SAMM50 Δ1–70 and N-terminal Myc tag | This study |
| pMXs puro-Myc SAMM50 Δ1–100 | Retroviral vector with Human SAMM50 Δ1–100 and N-terminal Myc tag | This study |
| pMXs puro-Myc SAMM50 Δ1–125 | Retroviral vector with Human SAMM50 Δ1–125 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 | Retroviral vector with Human SAMM50 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–40 | Retroviral vector with Human SAMM50 Δ1–40 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–70 | Retroviral vector with Human SAMM50 Δ1–70 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–100 | Retroviral vector with Human SAMM50 Δ1–100 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–125 | Retroviral vector with Human SAMM50 Δ1–125 and N-terminal Myc tag | This study |
| pMRXIP GFP-SAMM50 | Retroviral vector with Human SAMM50 and N-terminal GFP fusion tag | This study |
| pMRXIP GFP-SAMM50 Δ1–125 | Retroviral vector with Human SAMM50 Δ1–125 and N-terminal GFP fusion tag | This study |
| pDest15 LC3A | Human LC3A with N-terminal GST fusion tag | (Pankiv et al., 2007) |
| pDest15 LC3B | Human LC3B with N-terminal GST fusion tag | Pankiv et al., 2007 |
| pDest15 LC3C | Human LC3C with N-terminal GST fusion tag | (Pankiv et al., 2007) |
| pDest15 GABARAP | Human GABARAP with N-terminal GST fusion tag | (Pankiv et al., 2007) |
| pDest15 GABARAPL1 | Human GABARAPL1 with N-terminal GST fusion tag | (Pankiv et al., 2007) |
| pDest15 GABARAPL2 | HumanGABARAPL2 with N-terminal GST fusion tag | (Pankiv et al., 2007) |
| pDest15 GABARAP Y49A | Human GABARAP Y49A with N-terminal GST fusion tag | This study |
| pDONR221 SAMM50 F28A/V31A | Human SAMM50 with F28A/V31A point mutations in Gateway donor vector | This study |
| pDONR221 SAMM50 Δ28–31 | Human SAMM50 with 28–31 deletion in Gateway donor vector | This study |
| pDONR221 SAMM50 Δ24–35 | Human SAMM50 with 24–35 deletion in Gateway donor vector | This study |
| pDestMyc SAMM50 F28A/V31A | Human SAMM50 with F28A/V31A point mutations and N-terminal Myc tag | This study |
| pDestMyc SAMM50 Δ28–31 | Human SAMM50 with 28–31 deletion and N-terminal Myc tag | This study |
| pDestMyc SAMM50 Δ24–35 | Human SAMM50 with 24–35 deletion and N-terminal Myc tag | This study |
| pMXs-puro mCherry-EGFP OMP25TM | Retroviral vector with tandem tag mCherry-EGFP OMP25TM mitophagy reporter | This study |
| pMRXIP Myc-LC3A | Retroviral vector with Human LC3A and N-terminal Myc tag | This study |
| pMRXIP Myc-LC3B | Retroviral vector with Human LC3B and N-terminal Myc tag | This study |
| pMRXIP Myc-LC3C | Retroviral vector with Human LC3C and N-terminal Myc tag | This study |
| pMRXIP Myc-GABARAP | Retroviral vector with Human GABARAP and N-terminal Myc tag | This study |
| pMRXIP Myc-GABARAPL1 | Retroviral vector with Human GABARAPL1 and N-terminal Myc tag | This study |
| pMRXIP Myc-GABARAPL2 | Retroviral vector with Human GABARAPL2and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 | Retroviral vector with Human SAMM50 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–40 | Retroviral vector with Human SAMM50 Δ1–40 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–70 | Retroviral vector with Human SAMM50 Δ1–70 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–100 | Retroviral vector with Human SAMM50 Δ1–100 and N-terminal Myc tag | This study |
| pMRXIP Myc-SAMM50 Δ1–125 | Retroviral vector with Human SAMM50 Δ1–125 and N-terminal Myc tag | This study |
| pMRXIP COX8-EGFP-mCherry | Retroviral vector with tandem tag COX8-EGFP-mCherry mitophagy reporter | This study |
| pCDNA5/FRT-mNG1-10 | Flip-In vector with modified mNeonGreen β-sheet 1–10 (mNG1-10) | This study |
| pMRXIP-mNG11-SAMM50 | Retroviral vector with the Human SAMM50 and N-terminal mNG11 tag | This study |
| pMRXIP-mNG11-SAMM50 | Retroviral vector with the Human p62 and N-terminal mNG11 tag | This study |
| pCDNA5/FRT-MIC19-EGFP | Flip-In vector with Human MIC19 and C-terminal EGFP tag | This study |
| pMRXIP-MIC19-mCherry | Retroviral vector with Human MIC19 and C-terminal mCherry tag | This study |
| pCDNA5/FRT-LAMP1-mCherry | Flip-In vector with Human LAMP1 and C-terminal mCherry tag | This study |
| pMRXIP-LAMP1-EGFP | Retroviral vector with Human LAMP1 and C-terminal EGFP tag | This study |
| pMRXIP-EGFP-p62 | Retroviral vector with Human p62 and N-terminal EGFP tag | This study |
| pMRXIP-EGFP-LC3A | Retroviral vector with Human LC3A and N-terminal EGFP tag | This study |
| pMRXIP-EGFP-GABARAP | Retroviral vector with Human GABARAP and N-terminal EGFP tag | This study |
| pCDH-EF1α-GW-IRES-puromycin | Lentiviral vector with eukaryotic elongation factor 1 alpha (EF1α) promoter | System Biosciences |
| pCDH-EF1α-GW-IRES-puromycin-mCherry-p62 | Lentiviral vector with p62 and N-terminal mCherry tag | This study |
| pCDH-EF1α-GW-IRES-puromycin-Myc-p62 | Lentiviral vector with p62 and N-terminal Myc tag | This study |
| pCDH-EF1α-GW-IRES-puromycin-Myc-p62 Δ170–256 | Lentiviral vector with p62 Δ170-256 and N-terminal mCherry tag | This study |
| pCDH-EF1α-GW-IRES-puromycin-mCherry-ATG13 | Lentiviral vector with ATG13 and N-terminal mCherry tag | This study |
| pCDH-EF1α-GW-IRES-puromycin-mCherry-GABARAP | Lentiviral vector with GABARAP and N-terminal mCherry tag | This study |