Table 3.
Oligonucleotides used in this study for plasmid construction
Plasmids made in this studyOligonucleotides
pLexA-ERG6 Erg6.1D.EcoRI.TH 
AAC​CAA​CCG​AAT​TCA​TGA​GTG​AAA​CAG​AAT​TGA​GAA​AAA​GAC 
Erg6.1152U.XhoI.TH 
AAC​CAA​CCC​TCG​AGT​TAT​TGA​GTT​GCT​TCT​TGG​GAA​GTT​TGG 
pB42-ERG6 Erg6.1D.EcoRI.TH 
AAC​CAA​CCG​AAT​TCA​TGA​GTG​AAA​CAG​AAT​TGA​GAA​AAA​GAC 
Erg6.1152U.XhoI.TH 
AAC​CAA​CCC​TCG​AGT​TAT​TGA​GTT​GCT​TCT​TGG​GAA​GTT​TGG 
pB42-ERG27 Erg27.1D.EcoRI 
AAC​CAA​CCG​AAT​TCA​TGA​ACA​GGA​AAG​TAG​CTA​TCG​TAA​C 
Erg27.1043U.SalI 
AAC​CAA​CCG​TCG​ACT​TAA​ATG​GGG​GTT​CTA​GTT​TCA​ACA​ATT​TG 
pB42-OSH1 OSH1.1D.EcoRI 
AAC​CAA​GAA​TTC​ATG​TCT​TCG​TGC​TCT​CTG​CA 
OSH1.2580U.XhoI 
AAC​CAA​CTC​GAG​TTA​GAA​AAT​ATC​AGC​ACA​ATC​TTT​AAA​G 
pB42-OSH3ORD OSH3.1813D.MfeI 
AAC​CAA​CAA​TTG​AGC​GCC​CAA​TCC​TCA​AC 
OSH3.2991U.XhoI 
AAC​CAA​CTC​GAG​TCA​CCA​GAG​TTG​AGA​AAT​ATC​AGA​C 
pB42-OSH2ORD OSH2.2518D.EcoRI 
AAC​CAA​GAA​TTC​GTG​CAA​AAA​AGA​AAA​GAA​GAA​TAT​TTA​C 
OSH2.3840U.XhoI 
AAC​CAA​CTC​GAG​TTA​AAA​AAT​GTC​ACC​ACA​ATC​TTT​C 
pB42-OSH4 OSH4.1D.MfeI 
AAC​CAA​CAA​TTG​ATG​TCT​CAA​TAC​GCA​AGC​TCA​T 
OSH4.1302U.XhoI 
AAC​CAA​CTC​GAG​TTA​CAA​AAC​AAT​TTC​CTT​TTC​TTC 
pB42-OSH5 OSH5.1D.EcoRI 
AAC​CAA​GAA​TTC​ATG​TCT​CAA​CAC​GCA​AGC​TCA​TCT​TC 
OSH5.1305U.XhoI 
AAC​CAA​CTC​GAG​TTA​TAT​AGT​AAT​TTC​GTT​CTC​CCT​CAT​C 
pB42-OSH6 OSH6.1D.EcoRV.2 
AAC​CAA​GAT​ATC​AAT​GGG​CTC​CAA​AAA​ACT​GAC 
OSH6.1344U.XhoI 
AAC​CAA​CTC​GAG​CTA​TTG​TTT​TGC​TGG​GTT​CTG 
pB42-OSH7 OSH7.1D.SmaI.2 
AAC​CAA​CCC​GGG​AAT​GGC​TCT​CAA​TAA​ACT​AAA​GAA​TAT​AC 
OSH7.1314U.XhoI 
AAC​CAA​CTC​GAG​CTA​ATT​CTT​TTG​GAT​TCC​ATG​C 
p111osh2-KKK*-HA OSH2.-580D.EcoRI 
AAC​CAA​GAA​TTC​GAC​CTA​TAG​ATA​GTA​TGC​CCA​ATT​G 
Osh2.KKK-AAA.U 
TCC​TCC​TAG​GAT​CCA​CAC​CGC​CTG​TGC​TTT​CGC​GTT​GTA​GAC​TTC​GCC​TGT 
p111osh2-PPPVP*-ORD4-HA Osh2.Osh4.D 
CCA​TCT​ACT​ACA​AAA​AGA​GTT​GCG​AAA​CCT​TTG​AAC​CCA​TTT​CTA​GGT​GAG 
OSH2.3891U.R1 
ATA​CAA​GTA​CCA​GGA​AAA​AAG​CTC​GCA​TAA​AAA​AGG​CGT​GGA​ATT​CGA​GCT​CGT​TTA​AAC 
p33ERG6-OSH4-HA Erg6.linker.Osh4D 
CGC​CGA​AAC​CCC​CTC​CCA​AAC​TTC​CCA​AGA​AGC​AAC​TCA​AGC​AGG​GGC​AGC​AGG​GGC​AGC​AGC​AGC​AGG​GGC​AGC​AGG​GGC​AGC​AAT​GTC​TCA​ATA​CGC​AAG​CTC​ATC 
Erg6-R1 
AAA​TAG​GTA​TAT​ATC​GTG​CGC​TTT​ATT​TGA​ATC​TTA​TTG​ATC​TAG​TGA​ATG​AAT​TCG​AGC​TCG​TTT​AAA​C 
p111ERG6 Erg6.-500D.BamHI 
AAC​CAA​CCG​GAT​CCG​ATG​CAA​CAG​GGT​AAG​ATC​AG 
Erg6.TD200.EcoRI 
AAC​CAA​CCG​ATT​CTG​GTC​GTT​TGC​CAC​GAC​ATG 
p111erg6-D152L Erg6.-500D.BamHI 
AAC​CAA​CCG​GAT​TCG​ATG​CAA​CAG​GGT​AAG​ATC​AG 
Erg6.TD200.EcoRI 
AAC​CAA​CCG​AAT​TCT​GGT​CGT​TTG​CCA​CGA​CAT​G Erg6-D152L.D 
GTC​ATC​GGT​CTA​AAC​AAT​AAC​CTT​TAC​CAA​ATT​GCC​AAG​GCA​AAA​TAT 
Erg6-D152L.U 
ATA​TTT​TGC​CTT​GGC​AAT​TTG​GTA​AAG​GTT​ATT​GTT​TAG​ACC​GAT​GAC 
pGST-ORD2 OSH2.GST.ORD2.new.EcoRI 
AAC​CAA​GAA​TTC​CAA​AAG​AAA​GAA​GAA​TAT​TTA​CTC​AAA​G 
OSH2.3840U.XhoI 
AAC​CAA​CTC​GAG​TTA​AAA​AAT​GTC​ACC​ACA​ATC​TTT​C 
p33ERG6-OSH4-HA Erg6.linker.Osh4D 
CGC​CGA​AAC​CCC​CTC​CCA​AAC​TTC​CCA​AGA​AGC​AAC​TCA​AGC​AGG​GGC​AGC​AGG​GGC​AGC​AGC​AGC​AGG​GGC​AGC​AGG​GGC​AGC​AAT​GTC​TCA​ATA​CGC​AAG​CTC​ATC 
Osh4.R1 
ATT​AGT​GCA​ACG​GTA​ACA​AGT​TGT​TAC​TTT​ATC​GTT​CTC​CGA​ATT​CGA​GCT​CGT​TTA​AAC 
Plasmids made in this studyOligonucleotides
pLexA-ERG6 Erg6.1D.EcoRI.TH 
AAC​CAA​CCG​AAT​TCA​TGA​GTG​AAA​CAG​AAT​TGA​GAA​AAA​GAC 
Erg6.1152U.XhoI.TH 
AAC​CAA​CCC​TCG​AGT​TAT​TGA​GTT​GCT​TCT​TGG​GAA​GTT​TGG 
pB42-ERG6 Erg6.1D.EcoRI.TH 
AAC​CAA​CCG​AAT​TCA​TGA​GTG​AAA​CAG​AAT​TGA​GAA​AAA​GAC 
Erg6.1152U.XhoI.TH 
AAC​CAA​CCC​TCG​AGT​TAT​TGA​GTT​GCT​TCT​TGG​GAA​GTT​TGG 
pB42-ERG27 Erg27.1D.EcoRI 
AAC​CAA​CCG​AAT​TCA​TGA​ACA​GGA​AAG​TAG​CTA​TCG​TAA​C 
Erg27.1043U.SalI 
AAC​CAA​CCG​TCG​ACT​TAA​ATG​GGG​GTT​CTA​GTT​TCA​ACA​ATT​TG 
pB42-OSH1 OSH1.1D.EcoRI 
AAC​CAA​GAA​TTC​ATG​TCT​TCG​TGC​TCT​CTG​CA 
OSH1.2580U.XhoI 
AAC​CAA​CTC​GAG​TTA​GAA​AAT​ATC​AGC​ACA​ATC​TTT​AAA​G 
pB42-OSH3ORD OSH3.1813D.MfeI 
AAC​CAA​CAA​TTG​AGC​GCC​CAA​TCC​TCA​AC 
OSH3.2991U.XhoI 
AAC​CAA​CTC​GAG​TCA​CCA​GAG​TTG​AGA​AAT​ATC​AGA​C 
pB42-OSH2ORD OSH2.2518D.EcoRI 
AAC​CAA​GAA​TTC​GTG​CAA​AAA​AGA​AAA​GAA​GAA​TAT​TTA​C 
OSH2.3840U.XhoI 
AAC​CAA​CTC​GAG​TTA​AAA​AAT​GTC​ACC​ACA​ATC​TTT​C 
pB42-OSH4 OSH4.1D.MfeI 
AAC​CAA​CAA​TTG​ATG​TCT​CAA​TAC​GCA​AGC​TCA​T 
OSH4.1302U.XhoI 
AAC​CAA​CTC​GAG​TTA​CAA​AAC​AAT​TTC​CTT​TTC​TTC 
pB42-OSH5 OSH5.1D.EcoRI 
AAC​CAA​GAA​TTC​ATG​TCT​CAA​CAC​GCA​AGC​TCA​TCT​TC 
OSH5.1305U.XhoI 
AAC​CAA​CTC​GAG​TTA​TAT​AGT​AAT​TTC​GTT​CTC​CCT​CAT​C 
pB42-OSH6 OSH6.1D.EcoRV.2 
AAC​CAA​GAT​ATC​AAT​GGG​CTC​CAA​AAA​ACT​GAC 
OSH6.1344U.XhoI 
AAC​CAA​CTC​GAG​CTA​TTG​TTT​TGC​TGG​GTT​CTG 
pB42-OSH7 OSH7.1D.SmaI.2 
AAC​CAA​CCC​GGG​AAT​GGC​TCT​CAA​TAA​ACT​AAA​GAA​TAT​AC 
OSH7.1314U.XhoI 
AAC​CAA​CTC​GAG​CTA​ATT​CTT​TTG​GAT​TCC​ATG​C 
p111osh2-KKK*-HA OSH2.-580D.EcoRI 
AAC​CAA​GAA​TTC​GAC​CTA​TAG​ATA​GTA​TGC​CCA​ATT​G 
Osh2.KKK-AAA.U 
TCC​TCC​TAG​GAT​CCA​CAC​CGC​CTG​TGC​TTT​CGC​GTT​GTA​GAC​TTC​GCC​TGT 
p111osh2-PPPVP*-ORD4-HA Osh2.Osh4.D 
CCA​TCT​ACT​ACA​AAA​AGA​GTT​GCG​AAA​CCT​TTG​AAC​CCA​TTT​CTA​GGT​GAG 
OSH2.3891U.R1 
ATA​CAA​GTA​CCA​GGA​AAA​AAG​CTC​GCA​TAA​AAA​AGG​CGT​GGA​ATT​CGA​GCT​CGT​TTA​AAC 
p33ERG6-OSH4-HA Erg6.linker.Osh4D 
CGC​CGA​AAC​CCC​CTC​CCA​AAC​TTC​CCA​AGA​AGC​AAC​TCA​AGC​AGG​GGC​AGC​AGG​GGC​AGC​AGC​AGC​AGG​GGC​AGC​AGG​GGC​AGC​AAT​GTC​TCA​ATA​CGC​AAG​CTC​ATC 
Erg6-R1 
AAA​TAG​GTA​TAT​ATC​GTG​CGC​TTT​ATT​TGA​ATC​TTA​TTG​ATC​TAG​TGA​ATG​AAT​TCG​AGC​TCG​TTT​AAA​C 
p111ERG6 Erg6.-500D.BamHI 
AAC​CAA​CCG​GAT​CCG​ATG​CAA​CAG​GGT​AAG​ATC​AG 
Erg6.TD200.EcoRI 
AAC​CAA​CCG​ATT​CTG​GTC​GTT​TGC​CAC​GAC​ATG 
p111erg6-D152L Erg6.-500D.BamHI 
AAC​CAA​CCG​GAT​TCG​ATG​CAA​CAG​GGT​AAG​ATC​AG 
Erg6.TD200.EcoRI 
AAC​CAA​CCG​AAT​TCT​GGT​CGT​TTG​CCA​CGA​CAT​G Erg6-D152L.D 
GTC​ATC​GGT​CTA​AAC​AAT​AAC​CTT​TAC​CAA​ATT​GCC​AAG​GCA​AAA​TAT 
Erg6-D152L.U 
ATA​TTT​TGC​CTT​GGC​AAT​TTG​GTA​AAG​GTT​ATT​GTT​TAG​ACC​GAT​GAC 
pGST-ORD2 OSH2.GST.ORD2.new.EcoRI 
AAC​CAA​GAA​TTC​CAA​AAG​AAA​GAA​GAA​TAT​TTA​CTC​AAA​G 
OSH2.3840U.XhoI 
AAC​CAA​CTC​GAG​TTA​AAA​AAT​GTC​ACC​ACA​ATC​TTT​C 
p33ERG6-OSH4-HA Erg6.linker.Osh4D 
CGC​CGA​AAC​CCC​CTC​CCA​AAC​TTC​CCA​AGA​AGC​AAC​TCA​AGC​AGG​GGC​AGC​AGG​GGC​AGC​AGC​AGC​AGG​GGC​AGC​AGG​GGC​AGC​AAT​GTC​TCA​ATA​CGC​AAG​CTC​ATC 
Osh4.R1 
ATT​AGT​GCA​ACG​GTA​ACA​AGT​TGT​TAC​TTT​ATC​GTT​CTC​CGA​ATT​CGA​GCT​CGT​TTA​AAC 
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