| Plasmids . | Yeast features . | Insert . | Reference . |
|---|---|---|---|
| pRS316GFP-D4H | CEN4 URA3 | GFP-PFO-D4-D434S | Koselny et al., 2018 |
| pRS316GFP-D4H ura3Δ::LEU2 | CEN4 LEU2 | GFP-PFO-D4-D434S | This study |
| p RS316HA-GFP-D4H | CEN4 URA3 | 3HA-GFP-PFO-D4-D434S | This study |
| p111SLA2-GFP | CEN4 LEU2 HIS5 | SLA2-GFP | This study |
| p33ABP1-mCherry | CEN4 URA3 HIS3 | ABP1-mCherry | Encinar del Dedo et al., 2017 |
| p33ABP1-mCherry.ura3Δ::LEU2 | CEN4 LEU2 HIS3 | ABP1-mCherry | Encinar del Dedo et al., 2017 |
| p33PA-MYO5 | CEN4 URA3 | ProteinA-MYO5 | Grosshans et al., 2006 |
| p33MYO5 | CEN4 URA3 | MYO5 | Geli and Riezman, 1996 |
| pLexA-MYO5-tail | 2µ HIS3 | LexA-myo5(aa 757–1,219) | Geli et al., 2000 |
| pLexA-MYO5-TH2.SH3.CA | 2µ HIS3 | LexA-myo5(aa 984–1,219) | Grötsch et al., 2010 |
| pLexA-MYO5-SH3.CA | 2µ HIS3 | LexA-myo5(aa 1,085–1,219) | Grötsch et al., 2010 |
| pLexA-MYO5-CA | 2µ HIS3 | LexA-myo5(aa 1,142–1,219) | Grötsch et al., 2010 |
| pLexA-MYO5-TH1.TH2.SH3 | 2µ HIS3 | LexA-myo5(aa 757–1,181) | Grötsch et al., 2010 |
| pLexA-MYO5-TH1.TH2 | 2µ HIS3 | LexA-myo5(aa 757–1,091) | Grötsch et al., 2010 |
| pLexA-MYO5-TH1 | 2µ HIS3 | LexA-myo5(aa 757–996) | Grötsch et al., 2010 |
| pLexA-MYO5-TH2 | 2µ HIS3 | LexA-myo5(aa 984–1,091) | Grötsch et al., 2010 |
| pLexA-MYO5-SH3 | 2µ HIS3 | LexA-myo5(aa 1,085–1,181) | Grötsch et al., 2010 |
| pLexA-BCD1 | 2µ HIS3 | LexA-BCD1 | Gyuris et al., 1993 |
| pLexA-ERG6 | 2µ HIS3 | LexA-ERG6 | This study |
| pB42-ERG6 | 2µ TRP1 | B42-HA-ERG6 | This study |
| pB42-ERG27 | 2µ TRP1 | B42-HA-ERG27 | This study |
| pJG4-5 | 2µ TRP1 | B42-HA | Gyuris et al., 1993 |
| pB42-OSH1 | 2µ TRP1 | B42-OSH1 | This study |
| pB42-OSH2-N | 2µ TRP1 | B42-osh2(aa 1–860) | Encinar del Dedo et al., 2017 |
| pB42-OSH2-ORD | 2µ TRP1 | B42-osh2 (aa 840–1,240) | Encinar del Dedo et al., 2017 |
| pB42-OSH3-N | 2µ TRP1 | B42-osh2 (aa 1–604) | Encinar del Dedo et al., 2017 |
| pB42-OSH3ORD | 2µ TRP1 | B42-osh2(aa 605–997) | This study |
| pB42-OSH4 | 2µ TRP1 | B42-OSH4 | This study |
| pB42-OSH5 | 2µ TRP1 | B42-OSH5 | This study |
| pB42-OSH6 | 2µ TRP1 | B42-OSH6 | This study |
| pB42-OSH7 | 2µ TRP1 | B42-OSH7 | This study |
| Ycplac111 | CEN4 LEU2 | — | Gietz and Sugino, 1988 |
| Ycplac33 | CEN4 URA3 | — | Gietz and Sugino, 1988 |
| Ycp50 | CEN4 URA3 | — | Gietz and Sugino, 1988 |
| p111OSH2-HA | CEN4 LEU2 HIS3 | OSH2-HA | Encinar del Dedo et al., 2017 |
| p111osh2-ORDΔ-HA | CEN4 LEU2 HIS3 | osh2-ORDΔ-HA | Encinar del Dedo et al., 2017 |
| p111osh2-ORD4-HA | CEN4 LEU2 HIS3 | OSH2-ORD4-HA | Encinar del Dedo et al., 2017 |
| p111osh2-ORD6-HA | CEN4 LEU2 HIS3 | OSH2-ORD6-HA | Encinar del Dedo et al., 2017 |
| p111osh2-FFAT*-HA | CEN4 LEU2 HIS3 | osh2-FYD-AAA-HA | Encinar del Dedo et al., 2017 |
| p111osh2-HHR*-HA | CEN4 LEU2 HIS3 | osh2-HHR-AAA-HA | This study |
| p111osh2-KKK*-HA | CEN4 LEU2 HIS3 | osh2-KKK-AAA-HA | This study |
| p111osh2-PPPVP*-ORD4-HA | CEN4 LEU2 HIS3 | osh2-PPPVP-AAAA.ORD4-HA | This study |
| p33ERG6 | CEN4 URA3 | ERG6 | This study |
| p33ERG6-OSH4-HA | CEN4 URA3 HIS3 | ERG6-OSH4-HA | This study |
| pRS316OSH4-HA | CEN4 URA3 HIS3 | OSH4-HA | This study |
| p111ERG6 | CEN4 LEU2 | ERG6 | This study |
| p111erg6-D152L | CEN4 LEU2 | erg6-D152L | This study |
| p111OSH2-YFP | CEN4 LEU2 HIS3 | OSH2-YFP | Encinar del Dedo et al., 2017 |
| pGST-ORD2 | — | ORD OSH2 (aa 683–1,283) | This study |
| Plasmids . | Yeast features . | Insert . | Reference . |
|---|---|---|---|
| pRS316GFP-D4H | CEN4 URA3 | GFP-PFO-D4-D434S | Koselny et al., 2018 |
| pRS316GFP-D4H ura3Δ::LEU2 | CEN4 LEU2 | GFP-PFO-D4-D434S | This study |
| p RS316HA-GFP-D4H | CEN4 URA3 | 3HA-GFP-PFO-D4-D434S | This study |
| p111SLA2-GFP | CEN4 LEU2 HIS5 | SLA2-GFP | This study |
| p33ABP1-mCherry | CEN4 URA3 HIS3 | ABP1-mCherry | Encinar del Dedo et al., 2017 |
| p33ABP1-mCherry.ura3Δ::LEU2 | CEN4 LEU2 HIS3 | ABP1-mCherry | Encinar del Dedo et al., 2017 |
| p33PA-MYO5 | CEN4 URA3 | ProteinA-MYO5 | Grosshans et al., 2006 |
| p33MYO5 | CEN4 URA3 | MYO5 | Geli and Riezman, 1996 |
| pLexA-MYO5-tail | 2µ HIS3 | LexA-myo5(aa 757–1,219) | Geli et al., 2000 |
| pLexA-MYO5-TH2.SH3.CA | 2µ HIS3 | LexA-myo5(aa 984–1,219) | Grötsch et al., 2010 |
| pLexA-MYO5-SH3.CA | 2µ HIS3 | LexA-myo5(aa 1,085–1,219) | Grötsch et al., 2010 |
| pLexA-MYO5-CA | 2µ HIS3 | LexA-myo5(aa 1,142–1,219) | Grötsch et al., 2010 |
| pLexA-MYO5-TH1.TH2.SH3 | 2µ HIS3 | LexA-myo5(aa 757–1,181) | Grötsch et al., 2010 |
| pLexA-MYO5-TH1.TH2 | 2µ HIS3 | LexA-myo5(aa 757–1,091) | Grötsch et al., 2010 |
| pLexA-MYO5-TH1 | 2µ HIS3 | LexA-myo5(aa 757–996) | Grötsch et al., 2010 |
| pLexA-MYO5-TH2 | 2µ HIS3 | LexA-myo5(aa 984–1,091) | Grötsch et al., 2010 |
| pLexA-MYO5-SH3 | 2µ HIS3 | LexA-myo5(aa 1,085–1,181) | Grötsch et al., 2010 |
| pLexA-BCD1 | 2µ HIS3 | LexA-BCD1 | Gyuris et al., 1993 |
| pLexA-ERG6 | 2µ HIS3 | LexA-ERG6 | This study |
| pB42-ERG6 | 2µ TRP1 | B42-HA-ERG6 | This study |
| pB42-ERG27 | 2µ TRP1 | B42-HA-ERG27 | This study |
| pJG4-5 | 2µ TRP1 | B42-HA | Gyuris et al., 1993 |
| pB42-OSH1 | 2µ TRP1 | B42-OSH1 | This study |
| pB42-OSH2-N | 2µ TRP1 | B42-osh2(aa 1–860) | Encinar del Dedo et al., 2017 |
| pB42-OSH2-ORD | 2µ TRP1 | B42-osh2 (aa 840–1,240) | Encinar del Dedo et al., 2017 |
| pB42-OSH3-N | 2µ TRP1 | B42-osh2 (aa 1–604) | Encinar del Dedo et al., 2017 |
| pB42-OSH3ORD | 2µ TRP1 | B42-osh2(aa 605–997) | This study |
| pB42-OSH4 | 2µ TRP1 | B42-OSH4 | This study |
| pB42-OSH5 | 2µ TRP1 | B42-OSH5 | This study |
| pB42-OSH6 | 2µ TRP1 | B42-OSH6 | This study |
| pB42-OSH7 | 2µ TRP1 | B42-OSH7 | This study |
| Ycplac111 | CEN4 LEU2 | — | Gietz and Sugino, 1988 |
| Ycplac33 | CEN4 URA3 | — | Gietz and Sugino, 1988 |
| Ycp50 | CEN4 URA3 | — | Gietz and Sugino, 1988 |
| p111OSH2-HA | CEN4 LEU2 HIS3 | OSH2-HA | Encinar del Dedo et al., 2017 |
| p111osh2-ORDΔ-HA | CEN4 LEU2 HIS3 | osh2-ORDΔ-HA | Encinar del Dedo et al., 2017 |
| p111osh2-ORD4-HA | CEN4 LEU2 HIS3 | OSH2-ORD4-HA | Encinar del Dedo et al., 2017 |
| p111osh2-ORD6-HA | CEN4 LEU2 HIS3 | OSH2-ORD6-HA | Encinar del Dedo et al., 2017 |
| p111osh2-FFAT*-HA | CEN4 LEU2 HIS3 | osh2-FYD-AAA-HA | Encinar del Dedo et al., 2017 |
| p111osh2-HHR*-HA | CEN4 LEU2 HIS3 | osh2-HHR-AAA-HA | This study |
| p111osh2-KKK*-HA | CEN4 LEU2 HIS3 | osh2-KKK-AAA-HA | This study |
| p111osh2-PPPVP*-ORD4-HA | CEN4 LEU2 HIS3 | osh2-PPPVP-AAAA.ORD4-HA | This study |
| p33ERG6 | CEN4 URA3 | ERG6 | This study |
| p33ERG6-OSH4-HA | CEN4 URA3 HIS3 | ERG6-OSH4-HA | This study |
| pRS316OSH4-HA | CEN4 URA3 HIS3 | OSH4-HA | This study |
| p111ERG6 | CEN4 LEU2 | ERG6 | This study |
| p111erg6-D152L | CEN4 LEU2 | erg6-D152L | This study |
| p111OSH2-YFP | CEN4 LEU2 HIS3 | OSH2-YFP | Encinar del Dedo et al., 2017 |
| pGST-ORD2 | — | ORD OSH2 (aa 683–1,283) | This study |