Table 1.
Dynamic parameters of MTs measured at the cell cortex of LLCPK-1α cells stably expressing GFP-α-tubulin upon ectopic expression of WT or mutant (EBmut) mCherry-NAV1
Dynamic parametersmCherrymCh-NAV1-WTmCh-NAV1-EBmut
Growth    
Duration (s) 9.37 ± 6.47 8.78 ± 5.40 8.35 ± 4.92 
Rate (μm/min) 9.32 ± 3.65 8.69 ± 4.19 9.48 ± 3.96 
Shrinkage    
Duration (s) 2.46 ± 1.93 2.02 ± 1.60a 2.38 ± 1.82b 
Rate (μm/min) 42.33 ± 19.09 44.59 ± 26.69 41.92 ± 24.47 
Pause duration (s/min) 30.50 ± 11.26 37.23 ± 13.19c 36.13 ± 11.85c 
Transition frequencies    
Growth-shrinkage (min−1) 3.05 ± 2.11 3.50 ± 2.51 2.58 ± 2.24 
Growth-pause (min−1) 3.76 ± 2.58 4.19 ± 4.04 4.92 ± 2.89 
Shrinkage-growth (min−1) 19.02 ± 10.48 18.78 ± 12.98 20.88 ± 12.34 
Shrinkage-pause (min−1) 6.87 ± 8.12 13.29 ± 12.51a 6.10 ± 9.32b 
Pause-growth (min−1) 1.54 ± 1.32 1.49 ± 1.28 1.29 ± 1.28 
Pause-shrinkage (min−1) 2.27 ± 1.34 1.48 ± 1.29a 2.01 ± 1.72b 
Number of MTs analyzed 69 65 69 
Number of cells analyzed 30 27 29 

The average growth and shrinkage durations and rates are the average of all instantaneous durations and rates for the population of MTs. The average pause duration and transition frequencies are the average pause duration and transition frequencies for each MT. Values are means ± SD. Analyzed by two-tailed Student's t test.

a

Significant compared with mCherry-expressing cells (P < 0.001).

b

Nonsignificant compared with mCherry-expressing cells (P > 0.05) and significant compared with mCherry-NAV1-WT–expressing cells (P < 0.05).

c

Significant compared with mCherry-expressing cells (P < 0.01).

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