Table 1. List of proteins identified by... | Rockefeller University Press

Table 1.

List of proteins identified by MS after subcellular fractionation and K48-Ub immunoprecipitation using NEs as starting material

	Quantitative value (normalized total spectra)		Total unique peptide count		Percent coverage (%)
Protein name	4TorKO IP	WT IP	4TorKO IP	WT IP	4TorKO IP	WT IP
UBA52	49.16	35.03	11	11	50.00	50.00
ACTC1	33.09	27.60	25	16	47.20	44.30
HSPA1A	29.30	7.43	23	6	36.20	11.90
MLF2	7.56	2.12	8	2	35.90	12.10
HSPA8	48.21	25.48	27	20	34.80	33.60
VCP	29.30	31.84	26	29	34.60	38.20
Emerin	8.51	1.06	8	1	33.50	4.72
UBAC2	8.51	3.18	8	3	29.90	13.70
DNAJB2	8.51	1.06	6	1	27.80	3.09
DNAJB6	7.56	2.12	5	2	23.60	8.97
ERLIN2	7.56	11.68	6	6	22.40	22.40
ACTBL2	8.51	13.80	7	8	20.50	26.30
SARAF	8.51	0.00	5	0	19.20	0.00
VDAC1	5.67	9.55	5	7	18.70	33.60
APOA1	3.78	10.61	4	9	18.70	34.50
HSPA9	10.40	8.49	9	8	18.00	16.20
CANX	6.62	11.68	7	7	15.50	16.20
DNAJB12	5.67	0.00	5	0	13.60	0.00
PDE12	5.67	3.18	6	3	13.50	6.24
KCNG1	9.45	0.00	7	0	10.50	0.00

	Quantitative value (normalized total spectra)		Total unique peptide count		Percent coverage (%)
Protein name	4TorKO IP	WT IP	4TorKO IP	WT IP	4TorKO IP	WT IP
UBA52	49.16	35.03	11	11	50.00	50.00
ACTC1	33.09	27.60	25	16	47.20	44.30
HSPA1A	29.30	7.43	23	6	36.20	11.90
MLF2	7.56	2.12	8	2	35.90	12.10
HSPA8	48.21	25.48	27	20	34.80	33.60
VCP	29.30	31.84	26	29	34.60	38.20
Emerin	8.51	1.06	8	1	33.50	4.72
UBAC2	8.51	3.18	8	3	29.90	13.70
DNAJB2	8.51	1.06	6	1	27.80	3.09
DNAJB6	7.56	2.12	5	2	23.60	8.97
ERLIN2	7.56	11.68	6	6	22.40	22.40
ACTBL2	8.51	13.80	7	8	20.50	26.30
SARAF	8.51	0.00	5	0	19.20	0.00
VDAC1	5.67	9.55	5	7	18.70	33.60
APOA1	3.78	10.61	4	9	18.70	34.50
HSPA9	10.40	8.49	9	8	18.00	16.20
CANX	6.62	11.68	7	7	15.50	16.20
DNAJB12	5.67	0.00	5	0	13.60	0.00
PDE12	5.67	3.18	6	3	13.50	6.24
KCNG1	9.45	0.00	7	0	10.50	0.00

Proteins shown are the top 20 candidate proteins scored by highest percent sequence coverage in 4TorKO cell IP.

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