| Parameter . | Description . | Value . |
|---|---|---|
| r0 | Width of energy potential well characterizing the length of tubulin–tubulin bond | 0.13 nm |
| alat | Energy parameter for lateral repulsion; corresponds with ∼2 × height of the energy barrier for lateral bond | 15 kBT = 9 kcal/mol |
| blat | Depth of the potential well for lateral bonds | 5.3 kBT = 3.2 kcal/mol for MT growth |
| 4.3 kBT = 2.6 kcal/mol for MT shortening | ||
| along | Energy parameter for longitudinal repulsion; corresponds with ∼2 × height of the energy barrier for longitudinal interdimer bond | 15 kBT = 9 kcal/mol |
| blong | Depth of the potential well for longitudinal interdimer bond | 16.6 kBT = 10 kcal/mol |
| k | Stiffness of longitudinal intradimer bond | 517 kBT nm−2 = 310 kcal/mol nm−2 |
| B | Flexural rigidity of PF bending | 58 kBT rad−2 = 35 kcal/mol rad−2 |
| χD0 | Equilibrium angle between GDP-tubulin monomers | 0.2 rad |
| χT0 | Equilibrium angle between GTP-tubulin monomers | 0.2 rad |
| ctub | Soluble tubulin concentration | 10 µM |
| kon | On-rate constant for tubulin addition | 8.3 µM−1s−1 per MT |
| T | Temperature | 310 K |
| η | Viscosity | 0.2 Pa · s |
| dt | time step for dynamic algorithm | 10−10 s |
| Tkin | time step for kinetic algorithm | 10−3 s |
| Parameter . | Description . | Value . |
|---|---|---|
| r0 | Width of energy potential well characterizing the length of tubulin–tubulin bond | 0.13 nm |
| alat | Energy parameter for lateral repulsion; corresponds with ∼2 × height of the energy barrier for lateral bond | 15 kBT = 9 kcal/mol |
| blat | Depth of the potential well for lateral bonds | 5.3 kBT = 3.2 kcal/mol for MT growth |
| 4.3 kBT = 2.6 kcal/mol for MT shortening | ||
| along | Energy parameter for longitudinal repulsion; corresponds with ∼2 × height of the energy barrier for longitudinal interdimer bond | 15 kBT = 9 kcal/mol |
| blong | Depth of the potential well for longitudinal interdimer bond | 16.6 kBT = 10 kcal/mol |
| k | Stiffness of longitudinal intradimer bond | 517 kBT nm−2 = 310 kcal/mol nm−2 |
| B | Flexural rigidity of PF bending | 58 kBT rad−2 = 35 kcal/mol rad−2 |
| χD0 | Equilibrium angle between GDP-tubulin monomers | 0.2 rad |
| χT0 | Equilibrium angle between GTP-tubulin monomers | 0.2 rad |
| ctub | Soluble tubulin concentration | 10 µM |
| kon | On-rate constant for tubulin addition | 8.3 µM−1s−1 per MT |
| T | Temperature | 310 K |
| η | Viscosity | 0.2 Pa · s |
| dt | time step for dynamic algorithm | 10−10 s |
| Tkin | time step for kinetic algorithm | 10−3 s |