| Description . | Average ± SD (sample number) . | |||
|---|---|---|---|---|
| Control . | Act42A . | G15S . | tsr-RNAi . | |
| Dendrites / c4ddaC (number) | 187 ± 20 (n = 12) | 163 ± 37 (n = 9) | 105 ± 18 (n = 10) | 100 ± 20 (n = 10) |
| Dendrites / c3ddaF (number) | 263 ± 51 (n = 15) | 239 ± 41 (n = 9) | 64 ± 12 (n = 10) | 142 ± 21 (n = 10) |
| Dendrites / c3ddaA (number) | 329 ± 50 (n = 10) | 331 ± 60 (n = 9) | 72 ± 19 (n = 9) | 181± 48 (n = 8) |
| Dendrites / c1ddaE (MARCM; number) | 25 ± 6.6 (n = 11) | NDa | ND | 19.6 ± 2.9 (n = 10) |
| Dendrites / c1ddaE (number) | 23.8 ± 2.7 (n = 13) | 22.2 ± 3.1 (n = 8) | 12.6 ± 1.8 (n = 9) | ND |
| LifeAct intensity in proximal dendrites (AU) | 0.4 ± 0.1 (n = 9) | 0.4 ± 0.1 (n = 6) | 0.3 ± 0.2 (n = 8) | 0.4 ± 0.2 (n = 7) |
| LifeAct intensity in terminal dendrites (AU) | 0.5 ± 0.2 (n = 9) | 0.5 ± 0.1 (n = 6) | 0.3 ± 0.1 (n = 10) | 0.6 ± 0.3 (n = 7) |
| Dendrites with actin blobs (%) | 97.4 (n = 39) | 93.3 (n = 15) | 55.2 (n = 29) | 67.9 (n = 28) |
| Actin blobs in terminal dendrites (in 10 µm) | 3.3 ± 2.3 (n = 39) | 2.8 ± 1.8 (n = 15) | 0.5 ± 0.6 (n = 29) | 0.6 ± 0.6 (n = 28) |
| Dendrites with splitting events (%) | 42.1 (n = 38) | 33.3 (n = 15) | 7.1 (n = 14) | 0 (n = 28) |
| Velocity in terminal dendrites (μm/min) | 1.3 ± 0.4 (n = 94) | 1.4 ± 0.5 (n = 61) | 1.2 ± 0.4 (n = 23) | 1.1 ± 0.5 (n = 26) |
| Actin blob size (μm) | 3.1 ± 1.1 (n = 68) | 3.1 ± 1.4 (n = 22) | 1.8 ± 0.8 (n = 20) | 2.0 ± 0.9 (n = 23) |
| New dendrites (in 104 μm2) | 12.5 ± 7.4 (n = 14) | 12.2 ± 8.8 (n = 6) | 1.9 ± 2.3 (n = 12) | 3.1 ± 4.5 (n = 11) |
| New dendrites with prelocalization (%) | 83.0 (n = 53) | 78.9 (n = 19) | 33.3 (n = 18) | 47.4 (n = 19) |
| Normalized LifeAct intensity in new dendrites (AU) | 1.0 ± 0.4 (n = 30) | 1.0 ± 0.2 (n = 18) | 0.5 ± 0.3 (n = 21) | 0.6 ± 0.2 (n = 22) |
| New dendrite growth in 5 min (μm) | 3.8 ± 1.9 (n = 25) | 4.0 ± 1.4 (n = 20) | 2.5 ± 0.9 (n = 14) | 2.3 ± 0.9 (n = 13) |
| Existing dendrite extension in 10 min (μm) | 3.3 ± 0.5 (n = 81) | 3.4 ± 1.3 (n = 20) | 1.1 ± 0.2 (n = 48) | 1.9 ± 0.5 (n = 62) |
| Existing dendrite retraction in 10 min (μm) | 3.1 ± 0.9 (n = 81) | 3.8 ± 0.7 (n = 20) | 1.3 ± 0.4 (n = 48) | 2.0 ± 0.5 (n = 62) |
| Description . | Average ± SD (sample number) . | |||
|---|---|---|---|---|
| Control . | Act42A . | G15S . | tsr-RNAi . | |
| Dendrites / c4ddaC (number) | 187 ± 20 (n = 12) | 163 ± 37 (n = 9) | 105 ± 18 (n = 10) | 100 ± 20 (n = 10) |
| Dendrites / c3ddaF (number) | 263 ± 51 (n = 15) | 239 ± 41 (n = 9) | 64 ± 12 (n = 10) | 142 ± 21 (n = 10) |
| Dendrites / c3ddaA (number) | 329 ± 50 (n = 10) | 331 ± 60 (n = 9) | 72 ± 19 (n = 9) | 181± 48 (n = 8) |
| Dendrites / c1ddaE (MARCM; number) | 25 ± 6.6 (n = 11) | NDa | ND | 19.6 ± 2.9 (n = 10) |
| Dendrites / c1ddaE (number) | 23.8 ± 2.7 (n = 13) | 22.2 ± 3.1 (n = 8) | 12.6 ± 1.8 (n = 9) | ND |
| LifeAct intensity in proximal dendrites (AU) | 0.4 ± 0.1 (n = 9) | 0.4 ± 0.1 (n = 6) | 0.3 ± 0.2 (n = 8) | 0.4 ± 0.2 (n = 7) |
| LifeAct intensity in terminal dendrites (AU) | 0.5 ± 0.2 (n = 9) | 0.5 ± 0.1 (n = 6) | 0.3 ± 0.1 (n = 10) | 0.6 ± 0.3 (n = 7) |
| Dendrites with actin blobs (%) | 97.4 (n = 39) | 93.3 (n = 15) | 55.2 (n = 29) | 67.9 (n = 28) |
| Actin blobs in terminal dendrites (in 10 µm) | 3.3 ± 2.3 (n = 39) | 2.8 ± 1.8 (n = 15) | 0.5 ± 0.6 (n = 29) | 0.6 ± 0.6 (n = 28) |
| Dendrites with splitting events (%) | 42.1 (n = 38) | 33.3 (n = 15) | 7.1 (n = 14) | 0 (n = 28) |
| Velocity in terminal dendrites (μm/min) | 1.3 ± 0.4 (n = 94) | 1.4 ± 0.5 (n = 61) | 1.2 ± 0.4 (n = 23) | 1.1 ± 0.5 (n = 26) |
| Actin blob size (μm) | 3.1 ± 1.1 (n = 68) | 3.1 ± 1.4 (n = 22) | 1.8 ± 0.8 (n = 20) | 2.0 ± 0.9 (n = 23) |
| New dendrites (in 104 μm2) | 12.5 ± 7.4 (n = 14) | 12.2 ± 8.8 (n = 6) | 1.9 ± 2.3 (n = 12) | 3.1 ± 4.5 (n = 11) |
| New dendrites with prelocalization (%) | 83.0 (n = 53) | 78.9 (n = 19) | 33.3 (n = 18) | 47.4 (n = 19) |
| Normalized LifeAct intensity in new dendrites (AU) | 1.0 ± 0.4 (n = 30) | 1.0 ± 0.2 (n = 18) | 0.5 ± 0.3 (n = 21) | 0.6 ± 0.2 (n = 22) |
| New dendrite growth in 5 min (μm) | 3.8 ± 1.9 (n = 25) | 4.0 ± 1.4 (n = 20) | 2.5 ± 0.9 (n = 14) | 2.3 ± 0.9 (n = 13) |
| Existing dendrite extension in 10 min (μm) | 3.3 ± 0.5 (n = 81) | 3.4 ± 1.3 (n = 20) | 1.1 ± 0.2 (n = 48) | 1.9 ± 0.5 (n = 62) |
| Existing dendrite retraction in 10 min (μm) | 3.1 ± 0.9 (n = 81) | 3.8 ± 0.7 (n = 20) | 1.3 ± 0.4 (n = 48) | 2.0 ± 0.5 (n = 62) |
Not done.