Table 2.
List of antibodies used in this study
Antibody/ProbeSpeciesSourceProtocolDilutionFigure
Phospho-NMII (S19) Rabbit Cell Signaling no. 3671 Snap freezing, cardiac fixation 1:50 4A, S4 
Phospho-NMII (S19T18) Rabbit Cell Signaling no. 3674 Snap freezing, cardiac fixation 1:50 4A, S4 
NMIIA–heavy chain Rabbit BioLegend no. 909801 Snap freezing, cardiac fixation 1:50 4A, S2 
NMIIB–heavy chain Rabbit BioLegend no. 909901 Snap freezing, cardiac fixation 1:50 S2 
NMIIC–heavy chain Rabbit BioLegend 19192 Snap freezing, cardiac fixation 1:50 S3 
MLCK (Clone K36) Mouse Sigma-Aldrich Snap freezing, cardiac fixation 1:50 4A, S3 
Alexa 488–phalloidin, Alexa 594–phalloidin, Alexa 647–phalloidin  Molecular Probes Snap freezing, cardiac fixation, whole mount 1:200 2D, 3B, 4A, 4B, 4D 
Antibody/ProbeSpeciesSourceProtocolDilutionFigure
Phospho-NMII (S19) Rabbit Cell Signaling no. 3671 Snap freezing, cardiac fixation 1:50 4A, S4 
Phospho-NMII (S19T18) Rabbit Cell Signaling no. 3674 Snap freezing, cardiac fixation 1:50 4A, S4 
NMIIA–heavy chain Rabbit BioLegend no. 909801 Snap freezing, cardiac fixation 1:50 4A, S2 
NMIIB–heavy chain Rabbit BioLegend no. 909901 Snap freezing, cardiac fixation 1:50 S2 
NMIIC–heavy chain Rabbit BioLegend 19192 Snap freezing, cardiac fixation 1:50 S3 
MLCK (Clone K36) Mouse Sigma-Aldrich Snap freezing, cardiac fixation 1:50 4A, S3 
Alexa 488–phalloidin, Alexa 594–phalloidin, Alexa 647–phalloidin  Molecular Probes Snap freezing, cardiac fixation, whole mount 1:200 2D, 3B, 4A, 4B, 4D 
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