| Motor . | Track . | Actin glidingb . | Qdot ensemble motilityc . | Dual-color actin-gliding motilityd . |
|---|---|---|---|---|
| µm/s | µm/s | µm/s | ||
| Myo51 | Actin | 1.27 ± 0.19 (860) | 1.74 ± 0.50 (231) | Brownian motione |
| Myo51 | Actin-Tpm | 1.10 ± 0.14 (981) | 1.46 ± 0.46 (222) | Brownian motion |
| Myo51–Rng8/9 | Actin | 1.49 ± 0.25 (1,374) | 1.66 ± 0.49 (288) | Brownian motion |
| Myo51–Rng8/9 | Actin-Tpm | 0.06 ± 0.19 (1,515) | 0 | 1.28 ± 0.39 (114) |
| Motor . | Track . | Actin glidingb . | Qdot ensemble motilityc . | Dual-color actin-gliding motilityd . |
|---|---|---|---|---|
| µm/s | µm/s | µm/s | ||
| Myo51 | Actin | 1.27 ± 0.19 (860) | 1.74 ± 0.50 (231) | Brownian motione |
| Myo51 | Actin-Tpm | 1.10 ± 0.14 (981) | 1.46 ± 0.46 (222) | Brownian motion |
| Myo51–Rng8/9 | Actin | 1.49 ± 0.25 (1,374) | 1.66 ± 0.49 (288) | Brownian motion |
| Myo51–Rng8/9 | Actin-Tpm | 0.06 ± 0.19 (1,515) | 0 | 1.28 ± 0.39 (114) |
Data are mean ± SD; the number of tracked filaments or Qdots are given in parentheses.
Standard ensemble motility assay with motors immobilized on the surface.
Multiple motors attached to a Qdot moving on an immobilized track.
Green fluorescent–labeled actin or actin-Tpm immobilized on the surface as the substratum for ATP-insensitive motor attachment. Red fluorescent–labeled actin or actin-Tpm was flowed in to monitor motility.
Red filaments showed longitudinal Brownian motion but no directed motor-driven motion.