Table 1. Summary of GFA1-PP7 mRNP... | Rockefeller University Press

Table 1.

Summary of GFA1-PP7 mRNP imaging data

	REF	mex67-5
Particles tracked	291	203
Nuclear docking events	47	23
Export events	43	9
Retrograde export events	0	7
Mean export time—dwell time analysis (ms)	188^{^a}	ND
Mean export time—MLE (ms)	215^{^a}	ND
Mean nuclear docking time during export—dwell time analysis (ms)	32^{^a}	362^{^a}
Mean nuclear docking time during export—MLE (ms)	39^{^a}	202^{^a}
Mean transition time during export—dwell time analysis (ms)	87^{^a}	406^{^a}
Mean transition time during export—MLE (ms)	99^{^a}	383^{^a}
Mean cytoplasmic docking time during export—dwell time analysis (ms)	62^{^a}	1,258^{^a}
Mean cytoplasmic docking time during export—MLE (ms)	77^{^a}	943^{^a}
NE-associated mRNPs per cell	0.2 ± 0.4^{^b}	0.5 ± 0.7^{^b}
Nuclear scanning events	4	2
Cytoplasmic scanning events	102	16

	REF	mex67-5
Particles tracked	291	203
Nuclear docking events	47	23
Export events	43	9
Retrograde export events	0	7
Mean export time—dwell time analysis (ms)	188^a	ND
Mean export time—MLE (ms)	215^a	ND
Mean nuclear docking time during export—dwell time analysis (ms)	32^a	362^a
Mean nuclear docking time during export—MLE (ms)	39^a	202^a
Mean transition time during export—dwell time analysis (ms)	87^a	406^a
Mean transition time during export—MLE (ms)	99^a	383^a
Mean cytoplasmic docking time during export—dwell time analysis (ms)	62^a	1,258^a
Mean cytoplasmic docking time during export—MLE (ms)	77^a	943^a
NE-associated mRNPs per cell	0.2 ± 0.4^b	0.5 ± 0.7^b
Nuclear scanning events	4	2
Cytoplasmic scanning events	102	16

Export data underlying dwell time analysis may not have a normal distribution; consequently, an MLE analysis was performed (Kay, 1993). The differences between dwell times in REF and mex67-5 strains were tested using the distribution independent Wilcoxon rank-sum test and were found to be significant at P < 0.05 for the transition and cytoplasmic docking states. For more details and a discussion of error measurements, see the Materials and methods section Statistical analyses.

Standard deviation.

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