| Reactivity . | Epitope . | IF condition . | WB condition . | Species . | Reference or source . |
|---|---|---|---|---|---|
| aa | |||||
| Xenopus | |||||
| Bub1 | 274–467 | 5 µg/ml | 1.5 µg/ml | Rabbit | This study; Sharp-Baker and Chen, 2001 |
| BubR1 | 189–369 | 5 µg/ml | 1.5 µg/ml | Rabbit | This study; Chen, 2002 |
| CENP-A | 1–50 | 1.5 µg/ml | ND | Rabbit | This study; Maddox et al., 2003 |
| CENP-C | 207–296 | 1 µg/ml | 1 µg/ml | Rabbit | Milks et al., 2009 |
| CENP-E | 826–1,106 | 2 µg/ml | ND | Rabbit | Milks et al., 2009 |
| CENP-E | 2,397–2,954 | Rabbit | T. Lorca; Wood et al., 1997; Vigneron et al., 2004 | ||
| CENP-K | 276–287 | 2 µg/ml | ND | Rabbit | Milks et al., 2009 |
| CENP-T | 1–603 | 0.3 µg/ml | 0.3 µg/ml | Rabbit | A.F. Straight |
| Dasra A | 284–296 | 1.4 µg/ml | 7 µg/ml | Rabbit | Sampath et al., 2004) |
| dynein | Undiluted hybridoma supernatant | ND | Mouse | A.F. Straight | |
| H2A_T120ph | 1:100 | ND | Rabbit | Abcam (ab111492) | |
| H3_T3ph | ND | 1:5,000 | Rabbit | EMD Millipore (07-424) | |
| Mad1 | FL | 1:200 | ND | Rabbit | R.H. Chen; Chen et al., 1998 |
| Mad1 | 1–12 | Rabbit | T. Lorca; Vigneron et al., 2004 | ||
| Mad2 | FL | 1:500 | ND | Rabbit | Y. Mao; Chen et al., 1996; Mao et al., 2005 |
| Mps1 | 1–14 | 1:200 | ND | Rabbit | Y. Mao; Abrieu et al., 2001 |
| Mis12 | FL | 1:500 | 1:500 | Rabbit | Emanuele et al., 2005 |
| Ndc80 | FL | 1:500 | 1:1,000 | Rabbit | McCleland et al., 2003 |
| α-Tubulin | ND | 1:20,000 | Mouse | Sigma-Aldrich (T9026) | |
| Xorbit | 799–1,457 | 1:4,000 | ND | Rabbit | R. Heald; Hannak and Heald, 2006 |
| Zwint | FL | 1:500 | 1:1,000 | Rabbit | Emanuele et al., 2005 |
| Human | |||||
| ACA | CENP-A,B,C | 1:800 | ND | Human | Antibodies, Inc. (15-235) |
| BubR1 | 1:1,000 | ND | Rabbit | EMD Millipore (MAB3612) |
| Reactivity . | Epitope . | IF condition . | WB condition . | Species . | Reference or source . |
|---|---|---|---|---|---|
| aa | |||||
| Xenopus | |||||
| Bub1 | 274–467 | 5 µg/ml | 1.5 µg/ml | Rabbit | This study; Sharp-Baker and Chen, 2001 |
| BubR1 | 189–369 | 5 µg/ml | 1.5 µg/ml | Rabbit | This study; Chen, 2002 |
| CENP-A | 1–50 | 1.5 µg/ml | ND | Rabbit | This study; Maddox et al., 2003 |
| CENP-C | 207–296 | 1 µg/ml | 1 µg/ml | Rabbit | Milks et al., 2009 |
| CENP-E | 826–1,106 | 2 µg/ml | ND | Rabbit | Milks et al., 2009 |
| CENP-E | 2,397–2,954 | Rabbit | T. Lorca; Wood et al., 1997; Vigneron et al., 2004 | ||
| CENP-K | 276–287 | 2 µg/ml | ND | Rabbit | Milks et al., 2009 |
| CENP-T | 1–603 | 0.3 µg/ml | 0.3 µg/ml | Rabbit | A.F. Straight |
| Dasra A | 284–296 | 1.4 µg/ml | 7 µg/ml | Rabbit | Sampath et al., 2004) |
| dynein | Undiluted hybridoma supernatant | ND | Mouse | A.F. Straight | |
| H2A_T120ph | 1:100 | ND | Rabbit | Abcam (ab111492) | |
| H3_T3ph | ND | 1:5,000 | Rabbit | EMD Millipore (07-424) | |
| Mad1 | FL | 1:200 | ND | Rabbit | R.H. Chen; Chen et al., 1998 |
| Mad1 | 1–12 | Rabbit | T. Lorca; Vigneron et al., 2004 | ||
| Mad2 | FL | 1:500 | ND | Rabbit | Y. Mao; Chen et al., 1996; Mao et al., 2005 |
| Mps1 | 1–14 | 1:200 | ND | Rabbit | Y. Mao; Abrieu et al., 2001 |
| Mis12 | FL | 1:500 | 1:500 | Rabbit | Emanuele et al., 2005 |
| Ndc80 | FL | 1:500 | 1:1,000 | Rabbit | McCleland et al., 2003 |
| α-Tubulin | ND | 1:20,000 | Mouse | Sigma-Aldrich (T9026) | |
| Xorbit | 799–1,457 | 1:4,000 | ND | Rabbit | R. Heald; Hannak and Heald, 2006 |
| Zwint | FL | 1:500 | 1:1,000 | Rabbit | Emanuele et al., 2005 |
| Human | |||||
| ACA | CENP-A,B,C | 1:800 | ND | Human | Antibodies, Inc. (15-235) |
| BubR1 | 1:1,000 | ND | Rabbit | EMD Millipore (MAB3612) |
IF, immunofluorescence; WB, Western blotting.