Table 1.
Extract depletion conditions
AntibodyAntibody/100-µl beadsAntibody beads to deplete 50 µl extract
 µg µl 
Bub1 10 100 
BubR1 10 100 
CENP-C 1.8 16.7 
CENP-E 10 100 (2 rounds of 50) 
Haspin 7 100 (2 rounds of 50) 
INCENP 10 100 
Mad1 10 100 (2 rounds of 50) 
Sgo1&2 16 each 200 (2 rounds of 100) 
AntibodyAntibody/100-µl beadsAntibody beads to deplete 50 µl extract
 µg µl 
Bub1 10 100 
BubR1 10 100 
CENP-C 1.8 16.7 
CENP-E 10 100 (2 rounds of 50) 
Haspin 7 100 (2 rounds of 50) 
INCENP 10 100 
Mad1 10 100 (2 rounds of 50) 
Sgo1&2 16 each 200 (2 rounds of 100) 
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