Table 5. Plasmids used in the study... | Rockefeller University Press

Table 5.

Plasmids used in the study

Plasmids^{^a}	Genotype	Yeast marker	Source
pMC314^{^b}	GPDp-U1A-GFP	LEU2	P. Silver^{^c}; Brodsky and Silver, 2002
pMC313	GPDp-MFA2-U1Ax16-3′UTR of MFA2	URA3	This work
pSA01	GPDp-MFA2-mCHERRY- U1Ax16-3′UTR of MFA2	URA3	This work
pMC409	PGK1p-PGK1-U1Ax16-3′UTR of PGK1	URA3	R. Parker; Sheth and Parker, 2003
pMC414	DCP2p-DCP2-RFP	HIS3	This work
pMC413	LSM1p-LSM1-RFP	HIS3	This work
pMC389	PAB1p-PAB1-RFP	HIS3	This work
pMCB470	EDC3p-EDC3-mCh	TRP1	R. Parker; Buchan et al., 2008
pMC522	MET25p-MS2-mCh	HIS3	D. Raveh^{^d}; Lavut and Raveh, 2012
pMC475^{^b}	GPDp-MFA2-MS2 × 2	URA3	R. Parker; Sheth and Parker, 2003

Plasmids^{^a}	Genotype	Yeast marker	Source
pMC314^{^b}	GPDp-U1A-GFP	LEU2	P. Silver^{^c}; Brodsky and Silver, 2002
pMC313	GPDp-MFA2-U1Ax16-3′UTR of MFA2	URA3	This work
pSA01	GPDp-MFA2-mCHERRY- U1Ax16-3′UTR of MFA2	URA3	This work
pMC409	PGK1p-PGK1-U1Ax16-3′UTR of PGK1	URA3	R. Parker; Sheth and Parker, 2003
pMC414	DCP2p-DCP2-RFP	HIS3	This work
pMC413	LSM1p-LSM1-RFP	HIS3	This work
pMC389	PAB1p-PAB1-RFP	HIS3	This work
pMCB470	EDC3p-EDC3-mCh	TRP1	R. Parker; Buchan et al., 2008
pMC522	MET25p-MS2-mCh	HIS3	D. Raveh^{^d}; Lavut and Raveh, 2012
pMC475^{^b}	GPDp-MFA2-MS2 × 2	URA3	R. Parker; Sheth and Parker, 2003

All plasmids, unless otherwise indicated, contain CEN and therefore are present in one or two copies per cell.

Multicopy 2μ plasmid.

Harvard Medical School, Boston, MA.

Ben-Gurion University of Negev, Beer-Sheva, Israel.