Table 1. Phosphorylated residues within... | Rockefeller University Press

Table 1.

Phosphorylated residues within the nonhelical tail domain of endogenous MHCII-B identified by MS

Residues	Sequence	Phosphoresidue	Screening	IMAC
1930–1940	GGPISFSSSR	1935	+	+
1929–1940	RGGPISFSSSR	1935	+	+
1929–1940	RGGPISFSSSR	1937	−	+
1930–1940	GGPISFSSSR	1938	+	+
1929–1940	RGGPISFSSSR	1938	+	+
1930–1940	GGPISFSSSR	1939	+	+
1929–1940	RGGPISFSSSR	1939	+	+
1930–1940	GGPISFSSSR	*	−	+
1930–1940	RGGPISFSSSR	*	−	+
1958–1976	QLHLEGASLELSDDDTESK	1965	−	+
1958–1976	QLHLEGASLELSDDDTESK	1969	+	+
1958–1976	QLHLEGASLELSDDDTESK	1965 + 1969	+	+

Residues	Sequence	Phosphoresidue	Screening	IMAC
1930–1940	GGPISFSSSR	1935	+	+
1929–1940	RGGPISFSSSR	1935	+	+
1929–1940	RGGPISFSSSR	1937	−	+
1930–1940	GGPISFSSSR	1938	+	+
1929–1940	RGGPISFSSSR	1938	+	+
1930–1940	GGPISFSSSR	1939	+	+
1929–1940	RGGPISFSSSR	1939	+	+
1930–1940	GGPISFSSSR	*	−	+
1930–1940	RGGPISFSSSR	*	−	+
1958–1976	QLHLEGASLELSDDDTESK	1965	−	+
1958–1976	QLHLEGASLELSDDDTESK	1969	+	+
1958–1976	QLHLEGASLELSDDDTESK	1965 + 1969	+	+

Asterisks indicate diphosphorylated forms of the peptide, although the specific combination of phosphorylated sites is not distinguishable from the MS/MS spectra. Plus signs denote positive detection of the indicated phosphorylation; minus signs indicates negative (no) detection of the indicated phosphorylation. Bold letters indicate the phosphorylated residue that has been positively detected or not detected within each peptide.

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