Table 2. MT-activated ATPase of MD380... | Rockefeller University Press

Table 2.

MT-activated ATPase of MD380

Construct	k_cat		K_0.5MT
	Basal	MT activated
	s⁻¹ head⁻¹	s⁻¹ head⁻¹	μM
WT	3.7 ± 0.6	60.6 ± 4.0	27.2 ± 4.0
α-R403A	4.0 ± 0.1^a	ND	ND
α-E416A	3.9 ± 0.1^a	ND	ND
α-E415A	2.8 ± 0.6	59.1 ± 12.2	54.7 ± 18.7
β-D417A	2.6 ± 0.5	55.4 ± 12.3	67.2 ± 24.3
β-E421A	4.0 ± 0.3	64.0 ± 17.3	104.7 ± 41.0

Construct	k_cat		K_0.5MT
	Basal	MT activated
	s⁻¹ head⁻¹	s⁻¹ head⁻¹	μM
WT	3.7 ± 0.6	60.6 ± 4.0	27.2 ± 4.0
α-R403A	4.0 ± 0.1a	ND	ND
α-E416A	3.9 ± 0.1a	ND	ND
α-E415A	2.8 ± 0.6	59.1 ± 12.2	54.7 ± 18.7
β-D417A	2.6 ± 0.5	55.4 ± 12.3	67.2 ± 24.3
β-E421A	4.0 ± 0.3	64.0 ± 17.3	104.7 ± 41.0

Errors indicate the errors in curve fitting.

Mean ± SEM of all data points.