MT-activated ATPase of MD380
| Construct | kcat | K0.5MT | |
| Basal | MT activated | ||
| s−1 head−1 | s−1 head−1 | μM | |
| WT | 3.7 ± 0.6 | 60.6 ± 4.0 | 27.2 ± 4.0 |
| α-R403A | 4.0 ± 0.1a | ND | ND |
| α-E416A | 3.9 ± 0.1a | ND | ND |
| α-E415A | 2.8 ± 0.6 | 59.1 ± 12.2 | 54.7 ± 18.7 |
| β-D417A | 2.6 ± 0.5 | 55.4 ± 12.3 | 67.2 ± 24.3 |
| β-E421A | 4.0 ± 0.3 | 64.0 ± 17.3 | 104.7 ± 41.0 |
| Construct | kcat | K0.5MT | |
| Basal | MT activated | ||
| s−1 head−1 | s−1 head−1 | μM | |
| WT | 3.7 ± 0.6 | 60.6 ± 4.0 | 27.2 ± 4.0 |
| α-R403A | 4.0 ± 0.1a | ND | ND |
| α-E416A | 3.9 ± 0.1a | ND | ND |
| α-E415A | 2.8 ± 0.6 | 59.1 ± 12.2 | 54.7 ± 18.7 |
| β-D417A | 2.6 ± 0.5 | 55.4 ± 12.3 | 67.2 ± 24.3 |
| β-E421A | 4.0 ± 0.3 | 64.0 ± 17.3 | 104.7 ± 41.0 |
Errors indicate the errors in curve fitting.
Mean ± SEM of all data points.