Measured and simulated parameters are indicated with bold and nonbold text, respectively. D_1,mob determined by SPT is lower than D_sim established by simulations, as expected due to reduction in dimensionality from 3D to 2D. The decay time constant τ_B found by FCS reflects transient BRCA2 binding, as revealed by and in good agreement with SPT simulations. This transient binding behavior could not be directly measured by SPT because of the larger frame acquisition time of 50 ms. However, it produces and matches the diffusion constant D_2,mob very well. The theoretical diffusion parameters established by SPT simulations yielded the apparent diffusion constants D₁ and D₂ of 1.25 (53%) and 0.06 µm²/s after analyzing the synthetically generated data.