Table 2.

Antibody reactivity, dilution, and source

Antibody reactivity and host Dilution Source 
Nmr1/ H15 (guinea-pig and rabbit) 1:2,000 J. Skeath, Washington University in St. Louis, St. Louis, MO (Leal et al., 2009) 
Dg (rabbit) 1:2,000 W.-M. Deng, Florida State University, Tallahassee, FL (Deng et al., 2003) 
Zipper (rabbit) 1:1,000 D. Kiehart (Kiehart and Feghali, 1986) 
β-Galactosidase (mouse) 1:750 Promega 
β-Galactosidase (chicken) 1:500 Abcam 
Sex-lethal M18 (mouse) 1:10 DSHB (Bopp et al., 1991) 
Slit (mouse) 1:40 DSHB 
Discs-large1 (mouse) 1:25 DSHB 
Pericardin (mouse) 1:100 DSHB 
Trol (rabbit) 1:1,000 S. Baumgartner, Lund University, Lund, Sweden (Schneider et al., 2006) 
Multiplexin (rat) 1:100 T. Volk, Weizmann Institute of Science, Israel (Harpaz et al., 2013) 
dDaam (rabbit) 1:200 Matusek et al., 2006  
GFP (mouse) 1:500 Life Technologies 
GFP (chicken) 1:500 Aves Labs 
Antibody reactivity and host Dilution Source 
Nmr1/ H15 (guinea-pig and rabbit) 1:2,000 J. Skeath, Washington University in St. Louis, St. Louis, MO (Leal et al., 2009) 
Dg (rabbit) 1:2,000 W.-M. Deng, Florida State University, Tallahassee, FL (Deng et al., 2003) 
Zipper (rabbit) 1:1,000 D. Kiehart (Kiehart and Feghali, 1986) 
β-Galactosidase (mouse) 1:750 Promega 
β-Galactosidase (chicken) 1:500 Abcam 
Sex-lethal M18 (mouse) 1:10 DSHB (Bopp et al., 1991) 
Slit (mouse) 1:40 DSHB 
Discs-large1 (mouse) 1:25 DSHB 
Pericardin (mouse) 1:100 DSHB 
Trol (rabbit) 1:1,000 S. Baumgartner, Lund University, Lund, Sweden (Schneider et al., 2006) 
Multiplexin (rat) 1:100 T. Volk, Weizmann Institute of Science, Israel (Harpaz et al., 2013) 
dDaam (rabbit) 1:200 Matusek et al., 2006  
GFP (mouse) 1:500 Life Technologies 
GFP (chicken) 1:500 Aves Labs 

DSHB: antibodies were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by the Department of Biology at The University of Iowa.

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