Density of GC in A. punctulata sperm
| Parameters | Densitometry | MS (PSAQ) | ||
| α-Macroglobulin | Phosphorylase b | Recombinant GC | ||
| GC molecules/cell | 2.53 ± 1.22 × 105 (16) | 2.96 ± 1.65 × 105 (13) | 3.38 ± 2.79 × 105 (16) | 1.79 ± 0.33 × 105 (6) |
| GC density/micrometer squared | 7,200 ± 3,500 | 8,500 ± 4,700 | 9,700 ± 8,000 | 5,100 ± 900 |
| Occupancy of the flagellar surface | 16 ± 8% | 19 ± 10% | 21 ± 18% | 11 ± 2% |
| Parameters | Densitometry | MS (PSAQ) | ||
| α-Macroglobulin | Phosphorylase b | Recombinant GC | ||
| GC molecules/cell | 2.53 ± 1.22 × 105 (16) | 2.96 ± 1.65 × 105 (13) | 3.38 ± 2.79 × 105 (16) | 1.79 ± 0.33 × 105 (6) |
| GC density/micrometer squared | 7,200 ± 3,500 | 8,500 ± 4,700 | 9,700 ± 8,000 | 5,100 ± 900 |
| Occupancy of the flagellar surface | 16 ± 8% | 19 ± 10% | 21 ± 18% | 11 ± 2% |
Means ± SD. Numbers in parentheses indicate number of animals; each animal has been analyzed at least in duplicates. The density has been calculated assuming a surface area of 35 µm2. The occupancy of the flagellar surface has been calculated assuming a globular structure of the GC with a diameter of 5.3 nm.