Characterization of the poleward transport response in mechanically distinct systems
| Experimental condition | Poleward speed | Delay between end of ablation and response | Duration of poleward movement | Magnitude of poleward movement | Number of ablations (n) and number of cells (x) |
| µm/min | s | s | µm | ||
| Metaphase bipole ablation | 2.2 ± 0.2 | 15 ± 4 | 60 ± 7 | 2.1 ± 0.1 | n = 18, x = 17 |
| (n = 14, x = 13) | (n = 14, x = 13) | ||||
| Metaphase bipole control | 1.3 ± 0.2 | 56 ± 11a | 64 ± 11 | 1.4 ± 0.4 | n = 14, x = 10 |
| (n and x = 5) | (n and x = 5) | ||||
| Anaphase bipole ablation | 2.7 ± 0.3 | 18 ± 3 | 75 ± 10 | 3.0 ± 0.3 | n = 10, x = 6 |
| Anaphase bipole control | 0.8 ± 0.2 | n/ab | n/ab | n/ab | n = 10, x = 6 |
| Monopole ablation | 3.7 ± 0.3 | 8 ± 2 | 48 ± 7 | 2.2 ± 0.2 | n = 37, x = 19 |
| Monopole control | 1.5 ± 0.2 | 66 ± 17a | 53 ± 10 | 1.1 ± 0.2 | n = 7, x = 6 |
| Non-k-fiber microtubule bundles in monopoles | 13 ± 1 | 3 ± 1 | NDc | NDc | n = 12, x = 5 |
| Recruited GFP-Arp1A in bipoles | 2.6 ± 0.4 | 14 ± 3 | 32 ± 3 | 1.4 ± 0.2 | n = 8, x = 6 |
| Recruited GFP-NuMA in bipoles | 1.9 ± 0.1 | 8 ± 1 | 71 ± 6 | 2.0 ± 0.1 | n = 43, x = 14 |
| Recruited GFP-NuMA in monopoles | 2.9 ± 0.3 | 32 ± 2 | 37 ± 5 | 1.6 ± 0.2 | n = 23, x = 7 |
| DsRed-p150217-548 overexpression | n/ad | 122 ± 24 (post-ablation time without response) | n/ad | n/ad | n = 31, x = 11 |
| Experimental condition | Poleward speed | Delay between end of ablation and response | Duration of poleward movement | Magnitude of poleward movement | Number of ablations (n) and number of cells (x) |
| µm/min | s | s | µm | ||
| Metaphase bipole ablation | 2.2 ± 0.2 | 15 ± 4 | 60 ± 7 | 2.1 ± 0.1 | n = 18, x = 17 |
| (n = 14, x = 13) | (n = 14, x = 13) | ||||
| Metaphase bipole control | 1.3 ± 0.2 | 56 ± 11a | 64 ± 11 | 1.4 ± 0.4 | n = 14, x = 10 |
| (n and x = 5) | (n and x = 5) | ||||
| Anaphase bipole ablation | 2.7 ± 0.3 | 18 ± 3 | 75 ± 10 | 3.0 ± 0.3 | n = 10, x = 6 |
| Anaphase bipole control | 0.8 ± 0.2 | n/ab | n/ab | n/ab | n = 10, x = 6 |
| Monopole ablation | 3.7 ± 0.3 | 8 ± 2 | 48 ± 7 | 2.2 ± 0.2 | n = 37, x = 19 |
| Monopole control | 1.5 ± 0.2 | 66 ± 17a | 53 ± 10 | 1.1 ± 0.2 | n = 7, x = 6 |
| Non-k-fiber microtubule bundles in monopoles | 13 ± 1 | 3 ± 1 | NDc | NDc | n = 12, x = 5 |
| Recruited GFP-Arp1A in bipoles | 2.6 ± 0.4 | 14 ± 3 | 32 ± 3 | 1.4 ± 0.2 | n = 8, x = 6 |
| Recruited GFP-NuMA in bipoles | 1.9 ± 0.1 | 8 ± 1 | 71 ± 6 | 2.0 ± 0.1 | n = 43, x = 14 |
| Recruited GFP-NuMA in monopoles | 2.9 ± 0.3 | 32 ± 2 | 37 ± 5 | 1.6 ± 0.2 | n = 23, x = 7 |
| DsRed-p150217-548 overexpression | n/ad | 122 ± 24 (post-ablation time without response) | n/ad | n/ad | n = 31, x = 11 |
Data are presented as mean ± SEM. See also Figs. 1–3 and 5. n/a, not applicable.
Control delay time is the interval between the end of ablation and the first subsequent poleward motion of control unmanipulated chromosomes neighboring the ablation site.
Because anaphase movement is dominantly poleward, these control measurements are not applicable.
We did not measure duration and magnitude of the movement for non–k-fiber bundles since we were able to only briefly track the minus end after ablation.
Because we did not see any consistent poleward response in cells inhibited with the p150 fragment, these measurements are not applicable.