Genetic interactions of rng8Δ, rng9Δ, and myo51 mutations with other cytokinesis mutations
| Parent 1a | Parent 2a | Percentage of viable double mutants at 25°C | Total number of tetrads | Genetic interaction at 25°Cb |
| rng8Δ | myo2-E1 | 33 | 19 | +++ |
| rng8Δ | rlc1Δ | 43 | 36c | +++ |
| rng8Δ | myp2Δ | 100 | 24 | ++ |
| rng8Δ | myo2-ΔIQ1 ΔIQ2 | 100 | 17 | + |
| rng8Δ | ain1Δ | 100 | 18 | +d |
| rng8Δ | cdc3-124 | 100 | 17 | +e |
| rng8Δ | cdc8-110 | 100 | 16 | − |
| rng8Δ | clp1Δ | 100 | 12 | − |
| rng8Δ | mid1-6 | 100 | 12 | − |
| rng8Δ | mid1-366 | 100 | 11 | − |
| rng9Δ | myo2-E1 | 43 | 23 | +++ |
| rng9Δ | rlc1Δ | 25 | 19 | +++ |
| rng9Δ | myp2Δ | 100 | 18 | ++ |
| myo51Δ | myo2-E1 | 43 | 17 | +++ |
| myo51Δ | rlc1Δ | 30 | 21 | +++ |
| myo51Δ | myp2Δ | 96 | 20 | ++ |
| myo51Δ | ain1Δ | 100 | 22 | +d |
| myo51Δ | rng8Δ | 100 | 17 | − |
| myo51(753–1,471) | myo2-E1 | 44 | 19 | +++ |
| myo51(901–1,471) | myo2-E1 | 45 | 22 | +++ |
| myo51(1–1,294) | myo2-E1 | 78 | 20 | ++ |
| myo51(753–1,471) | myp2Δ | 100 | 18 | − |
| myo51(901–1,471) | myp2Δ | 100 | 14 | − |
| myo51(1–1,294) | myp2Δ | 100 | 20 | ++ |
| Parent 1a | Parent 2a | Percentage of viable double mutants at 25°C | Total number of tetrads | Genetic interaction at 25°Cb |
| rng8Δ | myo2-E1 | 33 | 19 | +++ |
| rng8Δ | rlc1Δ | 43 | 36c | +++ |
| rng8Δ | myp2Δ | 100 | 24 | ++ |
| rng8Δ | myo2-ΔIQ1 ΔIQ2 | 100 | 17 | + |
| rng8Δ | ain1Δ | 100 | 18 | +d |
| rng8Δ | cdc3-124 | 100 | 17 | +e |
| rng8Δ | cdc8-110 | 100 | 16 | − |
| rng8Δ | clp1Δ | 100 | 12 | − |
| rng8Δ | mid1-6 | 100 | 12 | − |
| rng8Δ | mid1-366 | 100 | 11 | − |
| rng9Δ | myo2-E1 | 43 | 23 | +++ |
| rng9Δ | rlc1Δ | 25 | 19 | +++ |
| rng9Δ | myp2Δ | 100 | 18 | ++ |
| myo51Δ | myo2-E1 | 43 | 17 | +++ |
| myo51Δ | rlc1Δ | 30 | 21 | +++ |
| myo51Δ | myp2Δ | 96 | 20 | ++ |
| myo51Δ | ain1Δ | 100 | 22 | +d |
| myo51Δ | rng8Δ | 100 | 17 | − |
| myo51(753–1,471) | myo2-E1 | 44 | 19 | +++ |
| myo51(901–1,471) | myo2-E1 | 45 | 22 | +++ |
| myo51(1–1,294) | myo2-E1 | 78 | 20 | ++ |
| myo51(753–1,471) | myp2Δ | 100 | 18 | − |
| myo51(901–1,471) | myp2Δ | 100 | 14 | − |
| myo51(1–1,294) | myp2Δ | 100 | 20 | ++ |
Single parent mutants were 95–100% viable on tetrad plates at 25°C.
Cells were grown exponentially in YE5S liquid medium before checking the morphology under DIC. The severity of cytokinesis defects compared to the parents (in terms of abnormal septation) was classified as: “+++,” very strong synthetic interaction and very severe cytokinesis defects; “++,” strong synthetic interaction and prominent cytokinesis defects; “+,” mild synthetic interaction and moderate cytokinesis defects; or “−,” no additive cytokinesis defects.
rng8 and rlc1 genes are tightly linked (∼34 kb) and only seven colonies were predicted double mutants in this cross.
The interaction was more obvious in EMM5S.
The interaction was more obvious at 28°C.