Quantitative evaluation of CRU and mitochondrial ultrastructure, and calcium signaling in SM derived from control and EtOH-fed rats
| Animal feeding | Ultrastructural measurements | Ca2+ signaling | ||||||||
| TT | jSR | TT-SR | Mitochondria | Mito-SR | [Ca2+]c | [Ca2+]m | ||||
| Area | Area | Gap | Contact length | Area | Aspect ratio | Gap | Contact length | Fura2 ΔR/R0 | mtRCaMP ΔF/F0 | |
| nm2 × 10−4 | nm2 × 10−4 | nm | % | nm2 × 10−4 | nm | nm | ||||
| Control | 0.24 ± 0.01 | 0.8 ± 0.07 | 19.9 ± 0.4 | 70.5 ± 1.2 | 3.6 ± 0.3 | 1.33 ± 0.02 | 25.1 ± 1 | 104 ± 6 | 1.93 ± 0.4 (n = 10) | 1.01 ± 0.3 (n = 6) |
| EtOH | 0.22 ± 0.01 | 0.76 ± 0.03 | 18.8 ± 0.4* | 68.6 ± 1.3 | 5.7 ± 0.5* | 1.26 ± 0.02** | 29.7 ± 1 | 83.4 ± 4** | 2.19 ± 0.2 (n = 11) | 0.84 ± 0.2 (n = 9) |
| Animal feeding | Ultrastructural measurements | Ca2+ signaling | ||||||||
| TT | jSR | TT-SR | Mitochondria | Mito-SR | [Ca2+]c | [Ca2+]m | ||||
| Area | Area | Gap | Contact length | Area | Aspect ratio | Gap | Contact length | Fura2 ΔR/R0 | mtRCaMP ΔF/F0 | |
| nm2 × 10−4 | nm2 × 10−4 | nm | % | nm2 × 10−4 | nm | nm | ||||
| Control | 0.24 ± 0.01 | 0.8 ± 0.07 | 19.9 ± 0.4 | 70.5 ± 1.2 | 3.6 ± 0.3 | 1.33 ± 0.02 | 25.1 ± 1 | 104 ± 6 | 1.93 ± 0.4 (n = 10) | 1.01 ± 0.3 (n = 6) |
| EtOH | 0.22 ± 0.01 | 0.76 ± 0.03 | 18.8 ± 0.4* | 68.6 ± 1.3 | 5.7 ± 0.5* | 1.26 ± 0.02** | 29.7 ± 1 | 83.4 ± 4** | 2.19 ± 0.2 (n = 11) | 0.84 ± 0.2 (n = 9) |
TEM samples from FDB muscle of three pairs for control vs. EtOH-fed animals, 21,000× images, from at least three fibers and a total of 32 measurements per sample, mean ± SE. *, P < 0.03; **, P < 0.006. Calcium signaling: amplitude of one Ca2+ transient triggered by field stimulation (100 Hz, 500 ms, 2-ms/pulse), mean ± SE.