Table 1. Effect of adenoviruses and... | Rockefeller University Press

Table 1.

Effect of adenoviruses and PKA-targeting drugs on the dispersal of membranous organelles

Organelle marker	Virus	Drug	Number of cells	Number of cells showing organelle dispersal	Cells showing organelle dispersal
					%
LysoTracker	−	−	31	5	16.1
LysoTracker	Ad5	−	46	39	84.8
LysoTracker	Ad5	H-89 (5 µM)	31	11	35.5
LysoTracker	Ad5	SB203580 (10 µM)	20	16	80.0
LysoTracker	Ad5	Cyclic RGD (8.5 µM)	15	6	40.0
LysoTracker	−	Forskolin (10 µM)	32	25	78.1
LysoTracker	Ad3	−	57	41	71.9
LysoTracker	Ad3	H-89 (5 µM)	32	5	15.6
GFP-Rab7	Ad5	−	18	12	66.7
GFP-Rab7	−	−	7	1	14.3
GFP-NPC1	Ad5	−	6	5	83.3
GFP-NPC1	−	−	4	0	0.0
GFP-NAGT	Ad5	−	13	0	0.0
GFP-NAGT	−	−	5	0	0.0
MitoTracker	Ad5	−	20	1	5.0
MitoTracker	−	−	4	0	0.0

Organelle marker	Virus	Drug	Number of cells	Number of cells showing organelle dispersal	Cells showing organelle dispersal
					%
LysoTracker	−	−	31	5	16.1
LysoTracker	Ad5	−	46	39	84.8
LysoTracker	Ad5	H-89 (5 µM)	31	11	35.5
LysoTracker	Ad5	SB203580 (10 µM)	20	16	80.0
LysoTracker	Ad5	Cyclic RGD (8.5 µM)	15	6	40.0
LysoTracker	−	Forskolin (10 µM)	32	25	78.1
LysoTracker	Ad3	−	57	41	71.9
LysoTracker	Ad3	H-89 (5 µM)	32	5	15.6
GFP-Rab7	Ad5	−	18	12	66.7
GFP-Rab7	−	−	7	1	14.3
GFP-NPC1	Ad5	−	6	5	83.3
GFP-NPC1	−	−	4	0	0.0
GFP-NAGT	Ad5	−	13	0	0.0
GFP-NAGT	−	−	5	0	0.0
MitoTracker	Ad5	−	20	1	5.0
MitoTracker	−	−	4	0	0.0

Dispersal was scored based on 90-min time-lapse videos. Minus signs indicate that either no virus or no drug was added.