Table 2. Genetic interactions of... | Rockefeller University Press

Table 2.

Genetic interactions of Δ503-cdc12 with cytokinesis mutants

Mutant^a	Viable double mutants at 25°C	Viable single mutants at 25°C^b	Total number of tetrads	Lowest synthetic lethal temperature^c
	%	%		°C
for3Δ	0	90	42	N/A
for3-I930A	0	42	43	N/A
mid1Δ	0	64	18	N/A
rng2-D5	7	89	11	30
rng2-346	0	88	11	N/A
cdc4-8	0	64	10	N/A
myo2-E1	5^d	82	19	N/A
rlc1Δ	0	93	11	N/A
rng3-65	100	100	10	30
cdc15-140	60	80	10	30
mid1-6	50	86	14	ND
mid1-366	67	100	9	ND
blt1Δ	93	100	10	ND

Mutant^a	Viable double mutants at 25°C	Viable single mutants at 25°C^b	Total number of tetrads	Lowest synthetic lethal temperature^c
	%	%		°C
for3Δ	0	90	42	N/A
for3-I930A	0	42	43	N/A
mid1Δ	0	64	18	N/A
rng2-D5	7	89	11	30
rng2-346	0	88	11	N/A
cdc4-8	0	64	10	N/A
myo2-E1	5^d	82	19	N/A
rlc1Δ	0	93	11	N/A
rng3-65	100	100	10	30
cdc15-140	60	80	10	30
mid1-6	50	86	14	ND
mid1-366	67	100	9	ND
blt1Δ	93	100	10	ND

Parent strains are given in Table S1.

Single mutants of Δ503-cdc12 were 93% viable on tetrad plates in this group of crosses. Ts single mutants were more viable after backcrossing; see Table 1.

Tested by streaking wt, single mutants, and double mutant on YE5S plates and incubating at temperatures from 25 to 36°C. The Δ503-cdc12 single mutants grew normally from 25 to 36°C.

The only viable double mutant isolated likely had a suppressor based on cell morphology at 36°C.

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