Time taken from nocodazole release for substrate levels to reach 20% of the starting level in control cells
| Substrate | Control siRNA | siMAD2L2 | siMAD2L2:hMAD2L2-myc |
| Cyclin B1 | 73.6 ± 2 | 32.1 ± 10.2 | 95.7 ± 20 |
| Securin | 69.7 ± 2.2 | 48.5 ± 12.6 | 57.9 ± 12 |
| Cdc20 | 118 ± 1.5 | 67.9 ± 12.2 | 122 ± 44.3 |
| AURKA | 179 ± 14.8 | 61.3 ± 15.3 | 180 ± 60.3 |
| UBCH10 | 248 ± 4.2 | 214 ± 55.8 | 234 ± 102 |
| MAD2L2 | 119 ± 1 | – | 251 ± 75 |
| Substrate | Control siRNA | siMAD2L2 | siMAD2L2:hMAD2L2-myc |
| Cyclin B1 | 73.6 ± 2 | 32.1 ± 10.2 | 95.7 ± 20 |
| Securin | 69.7 ± 2.2 | 48.5 ± 12.6 | 57.9 ± 12 |
| Cdc20 | 118 ± 1.5 | 67.9 ± 12.2 | 122 ± 44.3 |
| AURKA | 179 ± 14.8 | 61.3 ± 15.3 | 180 ± 60.3 |
| UBCH10 | 248 ± 4.2 | 214 ± 55.8 | 234 ± 102 |
| MAD2L2 | 119 ± 1 | – | 251 ± 75 |
Calculated from fitted exponential decay curves (Fig. 2 E).