List of antibodies used in this study
| Target | Mono/polyclonal | Clone/reference | Antibody raised in | Source | Dilution for I.B. | Dilution for I.F. |
| 53BP1 | Polyclonal | NB100-304 | Rabbit | Novus Biologicals | − | 1:800 |
| β-ACTIN | Monoclonal | 8226 | Mouse | Abcam | 1:5,000 | − |
| CHK1 | Monoclonal | G4 | Mouse | Santa Cruz Biotechnology, Inc. | 1:100 | − |
| CHK1 PhS345 | Monoclonal | 133D3 | Rabbit | Cell Signaling Technology | 1:1,000 | − |
| CHK2 | Polyclonal | ab8108 | Rabbit | Abcam | 1:2,000 | − |
| CHK2 PhT68 | Polyclonal | 2661 | Rabbit | Cell Signaling Technology | 1:500 | − |
| DNA-PKcs | Monoclonal | 18.2 | Mouse | Thermo Fisher Scientific | 1:200 | − |
| Fibrillarin | Monoclonal | AFB01 | Mouse | Universal Biologicals | − | 1:200 |
| GFP | Polyclonal | A11122 | Rabbit | Life Technologies | − | 1:2,000 |
| GFP | Monoclonal | 7.1 + 13.1 | Mouse | Roche | 1:2,000 | |
| H2AX | Polyclonal | ab11175 | Rabbit | Abcam | 1:4,000 | |
| γ-H2AX | Polyclonal | 2577 | Rabbit | Cell Signaling Technology | 1:500 | 1:100 |
| γ-H2AX | Monoclonal | JBW301 | Mouse | EMD Millipore | − | 1:1,000 |
| H3K9me3 | Polyclonal | ab8898 | Rabbit | Abcam | − | 1:500 |
| HA tag | Monoclonal | 12CA5 | Mouse | Home made | 1:2,000 | − |
| KAP-1 | Polyclonal | ab10483 | Rabbit | Abcam | 1:5,000 | − |
| KAP-1 PhS824 | Polyclonal | IHC-00073 | Rabbit | Bethyl Laboratories, Inc. | 1:200 | − |
| Ku70 | Monoclonal | N3H10 | Mouse | Abcam | 1:200 | − |
| Ku80 | Monoclonal | 111 | Mouse | Thermo Fisher Scientific | 1:2,000 | 1:100 |
| Ku80 (for MEF) | Polyclonal | C-20 | Goat | Santa Cruz Biotechnology, Inc. | 1:100 | − |
| Lamin A/C | Polyclonal | N-18 | Goat | Santa Cruz Biotechnology, Inc. | − | 1:200 |
| NBS1 | Polyclonal | NB100-143 | Rabbit | Novus Biologicals | 1:1,000 | 1:700 |
| Nucleolin/C23 | Polyclonal | H-250 | Rabbit | Santa Cruz Biotechnology, Inc. | 1:100 | |
| p53 | Monoclonal | 1C12 | Mouse | Cell Signaling Technology | 1:900 | − |
| PCNA | Polyclonal | ab18197 | Rabbit | Abcam | − | 1:1,000 |
| RAD51 | Polyclonal | H-92 | Rabbit | Santa Cruz Biotechnology, Inc. | − | 1:200 |
| RPA2/RPA32 | Monoclonal | 9H8 | Mouse | Abcam | 1:750 | 1:250 |
| XLF | Polyclonal | ab33499 | Rabbit | Abcam | 1:500 | − |
| XRCC4 | Polyclonal | ab145 | Rabbit | Abcam | 1:2,000 | − |
| Target | Mono/polyclonal | Clone/reference | Antibody raised in | Source | Dilution for I.B. | Dilution for I.F. |
| 53BP1 | Polyclonal | NB100-304 | Rabbit | Novus Biologicals | − | 1:800 |
| β-ACTIN | Monoclonal | 8226 | Mouse | Abcam | 1:5,000 | − |
| CHK1 | Monoclonal | G4 | Mouse | Santa Cruz Biotechnology, Inc. | 1:100 | − |
| CHK1 PhS345 | Monoclonal | 133D3 | Rabbit | Cell Signaling Technology | 1:1,000 | − |
| CHK2 | Polyclonal | ab8108 | Rabbit | Abcam | 1:2,000 | − |
| CHK2 PhT68 | Polyclonal | 2661 | Rabbit | Cell Signaling Technology | 1:500 | − |
| DNA-PKcs | Monoclonal | 18.2 | Mouse | Thermo Fisher Scientific | 1:200 | − |
| Fibrillarin | Monoclonal | AFB01 | Mouse | Universal Biologicals | − | 1:200 |
| GFP | Polyclonal | A11122 | Rabbit | Life Technologies | − | 1:2,000 |
| GFP | Monoclonal | 7.1 + 13.1 | Mouse | Roche | 1:2,000 | |
| H2AX | Polyclonal | ab11175 | Rabbit | Abcam | 1:4,000 | |
| γ-H2AX | Polyclonal | 2577 | Rabbit | Cell Signaling Technology | 1:500 | 1:100 |
| γ-H2AX | Monoclonal | JBW301 | Mouse | EMD Millipore | − | 1:1,000 |
| H3K9me3 | Polyclonal | ab8898 | Rabbit | Abcam | − | 1:500 |
| HA tag | Monoclonal | 12CA5 | Mouse | Home made | 1:2,000 | − |
| KAP-1 | Polyclonal | ab10483 | Rabbit | Abcam | 1:5,000 | − |
| KAP-1 PhS824 | Polyclonal | IHC-00073 | Rabbit | Bethyl Laboratories, Inc. | 1:200 | − |
| Ku70 | Monoclonal | N3H10 | Mouse | Abcam | 1:200 | − |
| Ku80 | Monoclonal | 111 | Mouse | Thermo Fisher Scientific | 1:2,000 | 1:100 |
| Ku80 (for MEF) | Polyclonal | C-20 | Goat | Santa Cruz Biotechnology, Inc. | 1:100 | − |
| Lamin A/C | Polyclonal | N-18 | Goat | Santa Cruz Biotechnology, Inc. | − | 1:200 |
| NBS1 | Polyclonal | NB100-143 | Rabbit | Novus Biologicals | 1:1,000 | 1:700 |
| Nucleolin/C23 | Polyclonal | H-250 | Rabbit | Santa Cruz Biotechnology, Inc. | 1:100 | |
| p53 | Monoclonal | 1C12 | Mouse | Cell Signaling Technology | 1:900 | − |
| PCNA | Polyclonal | ab18197 | Rabbit | Abcam | − | 1:1,000 |
| RAD51 | Polyclonal | H-92 | Rabbit | Santa Cruz Biotechnology, Inc. | − | 1:200 |
| RPA2/RPA32 | Monoclonal | 9H8 | Mouse | Abcam | 1:750 | 1:250 |
| XLF | Polyclonal | ab33499 | Rabbit | Abcam | 1:500 | − |
| XRCC4 | Polyclonal | ab145 | Rabbit | Abcam | 1:2,000 | − |
Minus signs indicate that the antibody was not used for this application in this study. PCNA, proliferating cell nuclear antigen; I.B., immunoblotting; I.F., immunofluorescence.