gRNA sequences, related to experimental procedures
| Cleavage site . | Guide ID . | Direction . | Sequences (5′-3′) . | PAM . |
|---|---|---|---|---|
| STX11 intron 1 | i1 | Forward | 5′-TGCACTTATTGCCCACA↓CCG-3′ | AGG |
| STX11 exon 2 | e1 | Forward | 5′-TGACCAGCAGTTCCCAG↓ACG-3′ | GGG |
| STX11 exon 2 | e2 | Reverse | 5′-TCGAACACGATGTCCTC ↓GTG-3′ | GGG |
| STX11 exon 2 | e3 | Reverse | 5′-CTTTCCCAGCCGCTTCA↓CGT-3′ | CGG |
| STX11 exon 2 | e4 | Forward | 5′-AGTCTCGGGCGACCAGA↓TCG-3′ | AGG |
| STX11 exon 2 | e5 | Reverse | 5′-GTTCTTCTCCTCGTACT↓GCA-3′ | CGG |
| Cleavage site . | Guide ID . | Direction . | Sequences (5′-3′) . | PAM . |
|---|---|---|---|---|
| STX11 intron 1 | i1 | Forward | 5′-TGCACTTATTGCCCACA↓CCG-3′ | AGG |
| STX11 exon 2 | e1 | Forward | 5′-TGACCAGCAGTTCCCAG↓ACG-3′ | GGG |
| STX11 exon 2 | e2 | Reverse | 5′-TCGAACACGATGTCCTC ↓GTG-3′ | GGG |
| STX11 exon 2 | e3 | Reverse | 5′-CTTTCCCAGCCGCTTCA↓CGT-3′ | CGG |
| STX11 exon 2 | e4 | Forward | 5′-AGTCTCGGGCGACCAGA↓TCG-3′ | AGG |
| STX11 exon 2 | e5 | Reverse | 5′-GTTCTTCTCCTCGTACT↓GCA-3′ | CGG |