Table 1.
Primers used in this study
Primer namePrimer sequence (5′–3′)
IQGAP1 shRNA KD1 3′UTR nt649 forwarda ACC​AGG​AGT​TAC​ACA​AAC​AGA​ATT​GCC​TTT​TTT​GAA​TTC​TCG​ACC​TCG​AGA​CAA​ATG​GCA​G 
IQGAP1 shRNA KD1 3′ UTR nt649 reversea ACC​TGG​AGT​TAC​ACA​AAC​AGA​ATT​GCC​CCC​GGT​GTT​TCG​TCC​TTT​CCA​CAA​GAT​ATA​TAA​AGC 
IQGAP1 shRNA KD2 3′UTR nt576 forwarda ACC​ACT​TCC​TTT​GAA​CAT​GAC​TGT​CAC​TTT​TTT​GAA​TTC​TCG​ACC​TCG​AGA​CAA​ATG​GCA​G 
IQGAP1 shRNA KD2 3′ UTR nt576 reversea ACC​TCT​TCC​TTT​GAA​CAT​GAC​TGT​CAC​CCC​GGT​GTT​TCG​TCC​TTT​CCA​CAA​GAT​ATA​TAA​AGC 
IQGAP1 mRNA amp forward ATGTCCGCCGCAGACGA 
IQGAP1 mRNA amp reverse TTA​CTT​CCC​GTA​GAA​CTT​TTT​GTT 
IQGAP1 nt1 SalI Kozak forward GCA​CGT​CGA​CGC​CAC​CAT​GTC​CGC​CGC​AGA​CGA 
IQGAP1 nt493 SalI Kozak forward GCA​CGT​CGA​CGC​CAC​CAT​GGC​CCC​TCA​GAT​TCA​AGA​CCT​ATA​TG 
IQGAP1 nt2190 XmaI reverse no stop TCT​CCC​CGG​GTG​ATA​GAA​CTC​TGG​ATC​TCC​TCC​C 
IQGAP1 nt2850 XmaI reverse no stop TCT​CCC​CGG​GAC​ATC​ATC​ATA​ATA​TCA​GAC​AAC​TGT​TC 
IQGAP1 nt4791 XmaI reverse no stop TCA​GCC​CGG​GAC​TTC​CCG​TAG​AAC​TTT​TTG​TTG 
IQGAP1 nt2188 Sal1 Kozak forward GCA​CGT​CGA​CGC​CAC​CAT​GTC​TGG​GGT​GAC​TGC​CGC​ATA​TAA​CC 
IQGAP1 nt2590 iqmot4 del forwarda CCT​CCT​ATG​GTT​GTG​GTC​CGA​AAA​TTT​GTC​C 
IQGAP1 nt2253 iqmot1 del reversea CCT​GGT​GAT​CAG​GCC​TTC​ATT​GGC​C 
PacI silent mutation forwarda GAG​GAT​TTT​AGC​CAT​TGG​TTT​GAT​TAA​TGA​AGC​CCT​GGA​TGA​AG 
PacI silent mutation reversea CTT​CAT​CCA​GGG​CTT​CAT​TAA​TCA​AAC​CAA​TGG​CTA​AAA​TCC​TC 
Primer namePrimer sequence (5′–3′)
IQGAP1 shRNA KD1 3′UTR nt649 forwarda ACC​AGG​AGT​TAC​ACA​AAC​AGA​ATT​GCC​TTT​TTT​GAA​TTC​TCG​ACC​TCG​AGA​CAA​ATG​GCA​G 
IQGAP1 shRNA KD1 3′ UTR nt649 reversea ACC​TGG​AGT​TAC​ACA​AAC​AGA​ATT​GCC​CCC​GGT​GTT​TCG​TCC​TTT​CCA​CAA​GAT​ATA​TAA​AGC 
IQGAP1 shRNA KD2 3′UTR nt576 forwarda ACC​ACT​TCC​TTT​GAA​CAT​GAC​TGT​CAC​TTT​TTT​GAA​TTC​TCG​ACC​TCG​AGA​CAA​ATG​GCA​G 
IQGAP1 shRNA KD2 3′ UTR nt576 reversea ACC​TCT​TCC​TTT​GAA​CAT​GAC​TGT​CAC​CCC​GGT​GTT​TCG​TCC​TTT​CCA​CAA​GAT​ATA​TAA​AGC 
IQGAP1 mRNA amp forward ATGTCCGCCGCAGACGA 
IQGAP1 mRNA amp reverse TTA​CTT​CCC​GTA​GAA​CTT​TTT​GTT 
IQGAP1 nt1 SalI Kozak forward GCA​CGT​CGA​CGC​CAC​CAT​GTC​CGC​CGC​AGA​CGA 
IQGAP1 nt493 SalI Kozak forward GCA​CGT​CGA​CGC​CAC​CAT​GGC​CCC​TCA​GAT​TCA​AGA​CCT​ATA​TG 
IQGAP1 nt2190 XmaI reverse no stop TCT​CCC​CGG​GTG​ATA​GAA​CTC​TGG​ATC​TCC​TCC​C 
IQGAP1 nt2850 XmaI reverse no stop TCT​CCC​CGG​GAC​ATC​ATC​ATA​ATA​TCA​GAC​AAC​TGT​TC 
IQGAP1 nt4791 XmaI reverse no stop TCA​GCC​CGG​GAC​TTC​CCG​TAG​AAC​TTT​TTG​TTG 
IQGAP1 nt2188 Sal1 Kozak forward GCA​CGT​CGA​CGC​CAC​CAT​GTC​TGG​GGT​GAC​TGC​CGC​ATA​TAA​CC 
IQGAP1 nt2590 iqmot4 del forwarda CCT​CCT​ATG​GTT​GTG​GTC​CGA​AAA​TTT​GTC​C 
IQGAP1 nt2253 iqmot1 del reversea CCT​GGT​GAT​CAG​GCC​TTC​ATT​GGC​C 
PacI silent mutation forwarda GAG​GAT​TTT​AGC​CAT​TGG​TTT​GAT​TAA​TGA​AGC​CCT​GGA​TGA​AG 
PacI silent mutation reversea CTT​CAT​CCA​GGG​CTT​CAT​TAA​TCA​AAC​CAA​TGG​CTA​AAA​TCC​TC 
a

These primers also contained a 5′ phosphate.

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