| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| APC BrdU Flow Kit | BD Biosciences | Cat#557892 |
| Donkey polyclonal anti-goat biotin | Jackson ImmunoResearch | Cat#705-065-003 |
| Donkey polyclonal anti-rabbit Alexa488 | Life Technologies | Cat#A21206 |
| Donkey polyclonal anti-rat Alexa488 | Jackson ImmunoResearch | Cat#112-545-003 |
| Donkey polyclonal anti-rat biotin | Jackson ImmunoResearch | Cat#112-065-003 |
| FITC BrdU Flow Kit | BD Biosciences | Cat#559619 |
| Goat polyclonal anti-GFP | Rockland | Cat#600-101-215 |
| Mouse monoclonal anti-human CD4 biotin | BD Biosciences | Clone RPA-T4; Cat#555345 |
| Mouse monoclonal anti-human CD4 PE | BD Biosciences | Clone L200; Cat#550630 |
| Mouse monoclonal anti-mouse FAK | Invitrogen | Clone ZF002; Cat#39-6500 |
| Rat monoclonal anti-CXCR4 biotin | eBioscience | Clone 2B11; Cat#13-9991-82 |
| Rat monoclonal anti-mouse CD19 BV711 | BioLegend | Clone 6D5; Cat#115555 |
| Rat monoclonal anti-mouse CD19 PE | BioLegend | Clone 6D5; Cat#115508 |
| Rat monoclonal anti-mouse CD93 APC | BioLegend | Clone AA4.1; Cat#136510 |
| Rat monoclonal anti-mouse cKIT BV421 | BioLegend | Clone ACK2; Cat#135124 |
| Rat monoclonal anti-mouse cKIT BV711 | BioLegend | Clone 2B8; Cat#105835 |
| Rat monoclonal anti-mouse IgD BV711 | BioLegend | Clone 11-26c.2A; Cat#405731 |
| Rat monoclonal anti-mouse IgD PE | BioLegend | Clone 11-26c.2A; Cat#405706 |
| Rat monoclonal anti-mouse IgG1 PE | BD Biosciences | Clone A85-1; Cat#550083 |
| Rat monoclonal anti-mouse IgM PECy7 | eBioscience | Clone II/41; Cat#25-5790-81 |
| Rat monoclonal anti-mouse Ikaros PE | BioLegend | Clone 2A9; Cat#653304 |
| Rat monoclonal anti-mouse Integrin α4 PE | Southern Biotech | Clone PS/2; Cat#1520-09L |
| Rat monoclonal anti-mouse pSTAT5 (Y694) BV421 | BD Biosciences | Clone 47/Stat5; Cat#562077 |
| Streptavidin Alexa700 | Life Technologies | Cat#S21383 |
| Streptavidin BV605 | BioLegend | Cat#405229 |
| Streptavidin BV711 | BioLegend | Cat#405241 |
| Bacterial and virus strains | ||
| “Subcloning efficiency” DH5α competent cells (F− Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk−, mk+) phoA supE44 thi-1 gyrA96 relA1 λ−) | Invitrogen | Cat#18265017 |
| Biological samples | ||
| N/A | ||
| Chemicals, peptides, and recombinant proteins | ||
| DMSO | American Bioanalytical | CAS:67-68-5 |
| Ibrutinib (PCI-32765) | Selleck Chemicals | Cat#S2680 |
| Fostamatinib (R788) | Selleck Chemicals | Cat#S2625 |
| Critical commercial assays | ||
| N/A | ||
| Deposited data | ||
| N/A | ||
| Experimental models: Cell lines | ||
| HEK293T | Gift from Dr. David Schatz, Yale University | |
| BCR-ABL | Schjerven et al., 2017 | Gift from Dr. Hilde Schjerven, University of California, San Francisco |
| Experimental models: Organisms/strains | ||
| Mus musculus, strain Mb1cre | Pelanda et al., 2002 | Gift from J.G. Cyster, University of California, San Francisco |
| Mus musculus, strain Il7rtm1.1Asin/J (Il7rFlox) | McCaughtry et al., 2012 | The Jackson Laboratory, Cat#022143 |
| Mus musculus, strain RAG2:GFP | Monroe et al., 1999 | Gift from Dr. David Schatz, Yale University |
| Mus musculus, strain Rag1−/− | Mombaerts et al., 1992 | Gift from Dr. David Schatz, Yale University |
| Mus musculus, strain Rag1GFP | Kuwata et al., 1999 | Our internal colony |
| Mus musculus, strain IghB1-8 (B6.129P2(C)-Ightm2Cgn/J) | Sonoda et al., 1997 | Gift from Dr. David Schatz, Yale University |
| Mus musculus, strain Igh3-83 (C.129P2(B6)-Igktm1Rsky Ightm2Rsky/PldJ) | Pelanda et al., 1997 | The Jackson Laboratory, Cat#011074 |
| Mus musculus, strain Il7GFP | Miller et al., 2013 | Gift from Dr. Susan Kaech, Yale University |
| Mus musculus, strain Cxcl12DsRed | Ding and Morrison, 2013 | Gift from Dr. Sean Morrison, University of Texas Southwestern Medical Center |
| Mus musculus, strain C57BL/6J | The Jackson Laboratory | Stock no. #000664 |
| Oligonucleotides | ||
| N/A | ||
| Recombinant DNA | ||
| pMXs-puro-EGFP-FAK | Hara et al., 2008 | Addgene plasmid #38194 |
| pMSCV CXCR4R334X IRES GFP | Pereira laboratory | N/A |
| pMSCV CXCR4R334X IRES hCD4 | This paper | N/A |
| Software and algorithms | ||
| FlowJo version 9.6.4 | Tree Star | http://www.flowjo.com |
| Imaris version 7.2 | Bitplane | http://www.bitplane.com/imaris/imaris |
| Other | ||
| N/A | ||
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| APC BrdU Flow Kit | BD Biosciences | Cat#557892 |
| Donkey polyclonal anti-goat biotin | Jackson ImmunoResearch | Cat#705-065-003 |
| Donkey polyclonal anti-rabbit Alexa488 | Life Technologies | Cat#A21206 |
| Donkey polyclonal anti-rat Alexa488 | Jackson ImmunoResearch | Cat#112-545-003 |
| Donkey polyclonal anti-rat biotin | Jackson ImmunoResearch | Cat#112-065-003 |
| FITC BrdU Flow Kit | BD Biosciences | Cat#559619 |
| Goat polyclonal anti-GFP | Rockland | Cat#600-101-215 |
| Mouse monoclonal anti-human CD4 biotin | BD Biosciences | Clone RPA-T4; Cat#555345 |
| Mouse monoclonal anti-human CD4 PE | BD Biosciences | Clone L200; Cat#550630 |
| Mouse monoclonal anti-mouse FAK | Invitrogen | Clone ZF002; Cat#39-6500 |
| Rat monoclonal anti-CXCR4 biotin | eBioscience | Clone 2B11; Cat#13-9991-82 |
| Rat monoclonal anti-mouse CD19 BV711 | BioLegend | Clone 6D5; Cat#115555 |
| Rat monoclonal anti-mouse CD19 PE | BioLegend | Clone 6D5; Cat#115508 |
| Rat monoclonal anti-mouse CD93 APC | BioLegend | Clone AA4.1; Cat#136510 |
| Rat monoclonal anti-mouse cKIT BV421 | BioLegend | Clone ACK2; Cat#135124 |
| Rat monoclonal anti-mouse cKIT BV711 | BioLegend | Clone 2B8; Cat#105835 |
| Rat monoclonal anti-mouse IgD BV711 | BioLegend | Clone 11-26c.2A; Cat#405731 |
| Rat monoclonal anti-mouse IgD PE | BioLegend | Clone 11-26c.2A; Cat#405706 |
| Rat monoclonal anti-mouse IgG1 PE | BD Biosciences | Clone A85-1; Cat#550083 |
| Rat monoclonal anti-mouse IgM PECy7 | eBioscience | Clone II/41; Cat#25-5790-81 |
| Rat monoclonal anti-mouse Ikaros PE | BioLegend | Clone 2A9; Cat#653304 |
| Rat monoclonal anti-mouse Integrin α4 PE | Southern Biotech | Clone PS/2; Cat#1520-09L |
| Rat monoclonal anti-mouse pSTAT5 (Y694) BV421 | BD Biosciences | Clone 47/Stat5; Cat#562077 |
| Streptavidin Alexa700 | Life Technologies | Cat#S21383 |
| Streptavidin BV605 | BioLegend | Cat#405229 |
| Streptavidin BV711 | BioLegend | Cat#405241 |
| Bacterial and virus strains | ||
| “Subcloning efficiency” DH5α competent cells (F− Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk−, mk+) phoA supE44 thi-1 gyrA96 relA1 λ−) | Invitrogen | Cat#18265017 |
| Biological samples | ||
| N/A | ||
| Chemicals, peptides, and recombinant proteins | ||
| DMSO | American Bioanalytical | CAS:67-68-5 |
| Ibrutinib (PCI-32765) | Selleck Chemicals | Cat#S2680 |
| Fostamatinib (R788) | Selleck Chemicals | Cat#S2625 |
| Critical commercial assays | ||
| N/A | ||
| Deposited data | ||
| N/A | ||
| Experimental models: Cell lines | ||
| HEK293T | Gift from Dr. David Schatz, Yale University | |
| BCR-ABL | Schjerven et al., 2017 | Gift from Dr. Hilde Schjerven, University of California, San Francisco |
| Experimental models: Organisms/strains | ||
| Mus musculus, strain Mb1cre | Pelanda et al., 2002 | Gift from J.G. Cyster, University of California, San Francisco |
| Mus musculus, strain Il7rtm1.1Asin/J (Il7rFlox) | McCaughtry et al., 2012 | The Jackson Laboratory, Cat#022143 |
| Mus musculus, strain RAG2:GFP | Monroe et al., 1999 | Gift from Dr. David Schatz, Yale University |
| Mus musculus, strain Rag1−/− | Mombaerts et al., 1992 | Gift from Dr. David Schatz, Yale University |
| Mus musculus, strain Rag1GFP | Kuwata et al., 1999 | Our internal colony |
| Mus musculus, strain IghB1-8 (B6.129P2(C)-Ightm2Cgn/J) | Sonoda et al., 1997 | Gift from Dr. David Schatz, Yale University |
| Mus musculus, strain Igh3-83 (C.129P2(B6)-Igktm1Rsky Ightm2Rsky/PldJ) | Pelanda et al., 1997 | The Jackson Laboratory, Cat#011074 |
| Mus musculus, strain Il7GFP | Miller et al., 2013 | Gift from Dr. Susan Kaech, Yale University |
| Mus musculus, strain Cxcl12DsRed | Ding and Morrison, 2013 | Gift from Dr. Sean Morrison, University of Texas Southwestern Medical Center |
| Mus musculus, strain C57BL/6J | The Jackson Laboratory | Stock no. #000664 |
| Oligonucleotides | ||
| N/A | ||
| Recombinant DNA | ||
| pMXs-puro-EGFP-FAK | Hara et al., 2008 | Addgene plasmid #38194 |
| pMSCV CXCR4R334X IRES GFP | Pereira laboratory | N/A |
| pMSCV CXCR4R334X IRES hCD4 | This paper | N/A |
| Software and algorithms | ||
| FlowJo version 9.6.4 | Tree Star | http://www.flowjo.com |
| Imaris version 7.2 | Bitplane | http://www.bitplane.com/imaris/imaris |
| Other | ||
| N/A | ||