Table I. B cell spreading and... | Rockefeller University Press

Table I.

B cell spreading and contraction response in DT40 B cell knockouts

DT40 knockout	Contact area (% of WT)	Antigen aggregation (% of WT)	No. of antigen microclusters (% of WT)
Lyn-KO	18 ± 1.4***	40 ± 2.1***	42 ± 4***
Syk-KO	18 ± 1***	57 ± 2.5***	36 ± 4***
Lyn/Syk-KO	13 ± 0.9***	ND	ND
Blnk-KO^{^a}	73 ± 3***	98 ± 3.3 (ns)	68 ± 5**
Blnk/Syk-KO	16 ± 1.5***	ND	ND
PLCγ2-KO	34 ± 2.2***	45 ± 2.4***	49 ± 4***
Btk-KO	52 ± 3.2***	81 ± 4.6*	69 ± 3**
Vav3-KO	39 ± 2.5***	63 ± 7.7*	70 ± 3**
BCAP-KO	76 ± 5.6**	129 ± 8.8 (ns)	ND
PI3K-KO	109 ± 4.7 (ns)	95 ± 8.7 (ns)	ND
IP₃R-KO	85 ± 3.1**	86 ± 5.6*	71 ± 6**
PKCβ-KO	88 ± 3.8**	82 ± 6.8*	74 ± 16**

DT40 knockout	Contact area (% of WT)	Antigen aggregation (% of WT)	No. of antigen microclusters (% of WT)
Lyn-KO	18 ± 1.4***	40 ± 2.1***	42 ± 4***
Syk-KO	18 ± 1***	57 ± 2.5***	36 ± 4***
Lyn/Syk-KO	13 ± 0.9***	ND	ND
Blnk-KO^{^a}	73 ± 3***	98 ± 3.3 (ns)	68 ± 5**
Blnk/Syk-KO	16 ± 1.5***	ND	ND
PLCγ2-KO	34 ± 2.2***	45 ± 2.4***	49 ± 4***
Btk-KO	52 ± 3.2***	81 ± 4.6*	69 ± 3**
Vav3-KO	39 ± 2.5***	63 ± 7.7*	70 ± 3**
BCAP-KO	76 ± 5.6**	129 ± 8.8 (ns)	ND
PI3K-KO	109 ± 4.7 (ns)	95 ± 8.7 (ns)	ND
IP₃R-KO	85 ± 3.1**	86 ± 5.6*	71 ± 6**
PKCβ-KO	88 ± 3.8**	82 ± 6.8*	74 ± 16**

B cells were settled on bilayers containing anti-IgM as antigen, and responses were visualized by confocal microscopy, IRM, or TIRFM. The responses of the various knockouts are classified by three parameters relative to WT: contact area (3 min), antigen aggregation (10 min), and number of microclusters (3 min). Data from at least two experiments are represented for each cell type, and each parameter was measured in 23–82 cells. ***, P < 0.0001; **, P < 0.005; *, P < 0.05. ND, not determined; ns, not significantly different.

Video 8 illustrates impaired spreading response in Blnk-DT40 cells.

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