Summary of the values of the temperature dependence obtained in the present in vitro motility assay experiments
| Myosin type . | Thin filament type . | pCa . | Q10 . | Ea (kJ/mol) . |
|---|---|---|---|---|
| Skeletal | F-actin | 9 | 1.4 ± 0.05 | 28.5 ± 2.8 |
| 5 | 1.4 ± 0.03 | 27.3 ± 1.9 | ||
| Skeletal | 9 | 8.2 ± 1.3 | 168 ± 13 | |
| 5 | 1.2 ± 0.06 | 17.6 ± 3.6 | ||
| Cardiac | 9 | 3.3 ± 0.3 | 94.3 ± 7.3 | |
| 5 | 1.3 ± 0.04 | 19.4 ± 2.7 | ||
| β-cardiac | F-actin | 9 | 3.5 ± 0.3 | 100 ± 7.7 |
| 5 | 3.1 ± 0.2 | 90.9 ± 4.6 | ||
| Skeletal | 9 | 30 ± 16 | 272 ± 41 | |
| 5 | 2.6 ± 0.1 | 76.6 ± 4.3 | ||
| Cardiac | 9 | 5.1 ± 1.2 | 130 ± 19 | |
| 5 | 1.5 ± 0.09 | 33.7 ± 4.6 |
| Myosin type . | Thin filament type . | pCa . | Q10 . | Ea (kJ/mol) . |
|---|---|---|---|---|
| Skeletal | F-actin | 9 | 1.4 ± 0.05 | 28.5 ± 2.8 |
| 5 | 1.4 ± 0.03 | 27.3 ± 1.9 | ||
| Skeletal | 9 | 8.2 ± 1.3 | 168 ± 13 | |
| 5 | 1.2 ± 0.06 | 17.6 ± 3.6 | ||
| Cardiac | 9 | 3.3 ± 0.3 | 94.3 ± 7.3 | |
| 5 | 1.3 ± 0.04 | 19.4 ± 2.7 | ||
| β-cardiac | F-actin | 9 | 3.5 ± 0.3 | 100 ± 7.7 |
| 5 | 3.1 ± 0.2 | 90.9 ± 4.6 | ||
| Skeletal | 9 | 30 ± 16 | 272 ± 41 | |
| 5 | 2.6 ± 0.1 | 76.6 ± 4.3 | ||
| Cardiac | 9 | 5.1 ± 1.2 | 130 ± 19 | |
| 5 | 1.5 ± 0.09 | 33.7 ± 4.6 |
The values of Q10 and Ea were determined from the sliding velocities between 33°C and 40°C (see Materials and methods). Data expressed as mean ± SEM.