Effects of various treatments on parameters calculated from dose–response relationships to 5-HT
| EC50 (nM) | Maximum 5-HT contraction amplitude (normalized to KCl) | Slope factor p | N | Mean R2 | |
|---|---|---|---|---|---|
| Wild-type | |||||
| Control | 209 ± 34 | 0.784 ± 0.073 | 1.57 ± 0.25 | 14 | 0.993 ± 0.004 |
| Nifedipine | 355 ± 117 | 0.451 ± 0.065* | 1.46 ± 0.33 | 5 | 0.997 ± 0.003 |
| CaCCInh-A01 | 673 ± 274 | 0.332 ± 0.059** | 1.33 ± 0.25 | 6 | 0.957 ± 0.019 |
| CPA | 309 ± 40 | 0.238 ± 0.045*** | 1.70 ± 0.44 | 4 | 0.996 ± 0.002 |
| Xestospongin C | 70 ± 10 | 0.294 ± 0.068*** | 1.93 ± 0.22 | 6 | 0.989 ± 0.003 |
| 0 Ca2+ | 211 ± 43 | 0.290 ± 0.060*** | 1.46 ± 0.30 | 11 | 0.986 ± 0.005 |
| 0 Ca2+ + CPA | 142 ± 19 | 0.306 ± 0.031*** | 1.12 ± 0.11 | 6 | 0.985 ± 0.011 |
| EC50 (nM) | Maximum 5-HT contraction amplitude (normalized to KCl) | Slope factor | Mean | ||
|---|---|---|---|---|---|
| Wild-type | |||||
| Control | 209 ± 34 | 0.784 ± 0.073 | 1.57 ± 0.25 | 14 | 0.993 ± 0.004 |
| Nifedipine | 355 ± 117 | 1.46 ± 0.33 | 5 | 0.997 ± 0.003 | |
| CaCCInh-A01 | 673 ± 274 | 1.33 ± 0.25 | 6 | 0.957 ± 0.019 | |
| CPA | 309 ± 40 | 1.70 ± 0.44 | 4 | 0.996 ± 0.002 | |
| Xestospongin C | 70 ± 10 | 1.93 ± 0.22 | 6 | 0.989 ± 0.003 | |
| 0 Ca2+ | 211 ± 43 | 1.46 ± 0.30 | 11 | 0.986 ± 0.005 | |
| 0 Ca2+ + CPA | 142 ± 19 | 1.12 ± 0.11 | 6 | 0.985 ± 0.011 |
Individual dose–response curves to 5-HT were least-square fitted to a logistic function of the following formalism: Y = A1 + [(A2 − A1)/(1 + ([5-HT]/EC50)p)], where Y is the contraction amplitude registered in the presence of 5-HT normalized to the high KCl-induced contraction, A1 and A2 are the minimum and maximum contraction amplitudes, respectively, [5-HT] is the concentration of 5-HT, EC50 is the concentration of 5-HT producing a contraction that is 50% of maximum, and p is the slope factor. Because of the biphasic nature of the dose–response curves to 5-HT (see Fig. 1 and text for explanation), only the initial portion of each curve, thus ranging from 0.01 to 1 or 3 μM 5-HT, was analyzed to calculate the parameters shown in the table. All values are means ± SEM pooled from 4 to 14 animals (N). One-way ANOVA tests revealed significant differences in the contraction amplitude between treatments and the control group (bolded numbers) with *P < 0.05, **P < 0.01, and ***P < 0.001. All other comparisons were not significant.
As a benefit of your subscription, you can share temporary access to restricted articles.
Each link will stop working after 30 days or 10 uses. You may create up to 10 links in a 30 day period.
Please sign in to your personal account to gift article access.
As a benefit of your subscription, you can share temporary access to restricted articles.
Each link will stop working after 30 days or 10 uses. You may create up to 10 links in a 30 day period.
Gift articles remaining: --
Each link will stop working after 30 days or 10 uses. You may create up to 10 links in a 30 day period.
Gift articles remaining: --
As a benefit of your subscription, you can share temporary access to restricted articles.
Each link will stop working after 30 days or 10 uses.
You have reached the limit of 10 links within a 30 day period.
Sharing content requires targeting cookies to be enabled. Please update your cookie preferences to use this feature.