Table 1.

Primers for murine mTMEM16A mutagenesis

F601A Forward 5′-TAT​GTC​GCC​GCC​TTC​AAA​GGC​CGG​T-3′ 
F601A Reverse 5′-GCC​TTT​GAA​GGC​GGC​GAC​ATA​GAA​G-3′ 
F601W Forward 5′-ATG​TCG​CCT​GGT​TCA​AAG​GCC​GGT-3′ 
F601W Reverse 5′-GGC​CTT​TGA​ACC​AGG​CGA​CAT​AGA​AGA​TG-3′ 
R605A Forward 5′-TTC​AAA​GGC​GCC​TTT​GTT​GGT​CGG-3′ 
R605A Reverse 5′-CCA​ACA​AAG​GCG​CCT​TTG​AAG​AAG​GC-3′ 
R605W Forward 5′-CTT​CAA​AGG​CTG​GTT​TGT​TGG​TCG-3′ 
R605W Reverse 5′-CCA​ACA​AAC​CAG​CCT​TTG​AAG​AAG​GC-3′ 
R605E Forward 5′-CTT​CAA​AGG​CGA​GTT​TGT​TGG​TCG​G-3′ 
R605E Reverse 5′-ACC​AAC​AAA​CTC​GCC​TTT​GAA​GAA​GGC-3′ 
F601A-R605A (R605A as template) Forward 5′-TAT​GTC​GCC​GCC​TTC​AAA​GGC​GC-3′ 
F601A-R605A (R605A as template) Reverse 5′-GCC​TTT​GAA​GGC​GGC​GAC​ATA​GAA​G-3′ 
F781A Forward 5′-TGT​GAT​CTC​CGC​CAC​GTC​TGA​CTT​C-3′ 
F781A Reverse 5′-CAG​ACG​TGG​CGG​AGA​TCA​CAA​AGG​C-3′ 
R864A Forward 5′-TGG​CCG​CCG​CAC​TGG​CAT​TTG​TCA​T-3′ 
R864A Reverse 5′-ATG​CCA​GTG​CGG​CGG​CCA​GGA​C-3′ 
F601A Forward 5′-TAT​GTC​GCC​GCC​TTC​AAA​GGC​CGG​T-3′ 
F601A Reverse 5′-GCC​TTT​GAA​GGC​GGC​GAC​ATA​GAA​G-3′ 
F601W Forward 5′-ATG​TCG​CCT​GGT​TCA​AAG​GCC​GGT-3′ 
F601W Reverse 5′-GGC​CTT​TGA​ACC​AGG​CGA​CAT​AGA​AGA​TG-3′ 
R605A Forward 5′-TTC​AAA​GGC​GCC​TTT​GTT​GGT​CGG-3′ 
R605A Reverse 5′-CCA​ACA​AAG​GCG​CCT​TTG​AAG​AAG​GC-3′ 
R605W Forward 5′-CTT​CAA​AGG​CTG​GTT​TGT​TGG​TCG-3′ 
R605W Reverse 5′-CCA​ACA​AAC​CAG​CCT​TTG​AAG​AAG​GC-3′ 
R605E Forward 5′-CTT​CAA​AGG​CGA​GTT​TGT​TGG​TCG​G-3′ 
R605E Reverse 5′-ACC​AAC​AAA​CTC​GCC​TTT​GAA​GAA​GGC-3′ 
F601A-R605A (R605A as template) Forward 5′-TAT​GTC​GCC​GCC​TTC​AAA​GGC​GC-3′ 
F601A-R605A (R605A as template) Reverse 5′-GCC​TTT​GAA​GGC​GGC​GAC​ATA​GAA​G-3′ 
F781A Forward 5′-TGT​GAT​CTC​CGC​CAC​GTC​TGA​CTT​C-3′ 
F781A Reverse 5′-CAG​ACG​TGG​CGG​AGA​TCA​CAA​AGG​C-3′ 
R864A Forward 5′-TGG​CCG​CCG​CAC​TGG​CAT​TTG​TCA​T-3′ 
R864A Reverse 5′-ATG​CCA​GTG​CGG​CGG​CCA​GGA​C-3′ 
[ViewLarge]
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal