Table 1.
WGA-pHRho labeling of various cells
ConditionsCell linesCardiomyocytesNeuron–astrocyte cocultures
[WGA-pHRho]final (mg/ml) 50  50  100  
Buffer HBSS HBSS GBFM 
Time (min) 10 30 20 
Temperature RT RT RT 
ConditionsCell linesCardiomyocytesNeuron–astrocyte cocultures
[WGA-pHRho]final (mg/ml) 50  50  100  
Buffer HBSS HBSS GBFM 
Time (min) 10 30 20 
Temperature RT RT RT 

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