Table 2. Properties of P2X4-pHluorin... | Rockefeller University Press

Table 2.

Properties of P2X4-pHluorin receptors expressed in HEK-293 cells

Construct	Position region	Fluorescence intensity	GFP/β actin ratio	E_rev	Rectification index	I_ATP	Surface fraction	Acidic fraction	Other fluorescence
		a.u.		mV		−pA	%	%	%
WT P2X4	—	—	—	−0.1 ± 0.8	0.79 ± 0.08	2,351 ± 315	—	—	—
				(n = 14)	(n = 14)	(n = 17)
P2X4-YFP	C tail	738 ± 62	1.0 ± 0.05	—	—	—	—	—	—
		(n = 9)	(n = 3)
P2X4-pHluorin79	Head	163 ± 17	0.9 ± 0.1	ND	ND	24 ± 11	42 ± 2	41.2 ± 4.1	16.8 ± 2.3
		(n = 16)	(n = 3)			(n = 8)	(n = 11)	(n = 11)	(n = 11)
P2X4-pHluorin82	Head	188 ± 28	1.8 ± 0.03	2.4 ± 1.2	0.53 ± 0.03	587 ± 155	38 ± 3	52.2 ± 3.4	9.5 ± 1.9
		(n = 13)	(n = 3)	(n = 7)	(n = 7)	(n = 8)	(n = 11)	(n = 11)	(n = 11)
P2X4-pHluorin123	Beak	359 ± 25	2.0 ± 0.2	0.4 ± 0.7	0.67 ± 0.09	1,853 ± 271	38 ± 3	50.8 ± 3.1	11.2 ± 2.4
		(n = 14)	(n = 3)	(n = 9)	(n = 9)	(n = 10)	(n = 14)	(n = 14)	(n = 14)
P2X4-pHluorin156	Beak	343 ± 31	1.9 ± 0.2	−0.03 ± 0.90	0.47 ± 0.04	472 ± 81	28 ± 3	51.6 ± 3.2	20.4 ± 3.2
		(n = 16)	(n = 3)	(n = 7)	(n = 8)	(n = 9)	(n = 12)	(n = 12)	(n = 12)
P2X4-pHluorin207	Dorsal fin	240 ± 9	1.5 ± 0.1	ND	ND	0.4 ± 0.4	13 ± 2	33.5 ± 5.1	53.1 ± 5.6
		(n = 7)	(n = 3)			(n = 3)	(n = 6)	(n = 6)	(n = 6)
P2X4-pHluorin222	Dorsal fin	120 ± 10	0.9 ± 0.1	ND	ND	0.9 ± 1.1	22 ± 2	20.7 ± 5.2	57.5 ± 3.5
		(n = 6)	(n = 3)			(n = 5)	(n = 5)	(n = 5)	(n = 5)
P2X4-pHluorin224	Dorsal fin	199 ± 25	0.9 ± 0.1	ND	ND	5 ± 4	14 ± 5	36.0 ± 9.6	49.7 ± 9.5
		(n = 9)	(n = 3)			(n = 5)	(n = 6)	(n = 6)	(n = 6)
P2X4-pHluorin304	Head	177 ± 19	1.9 ± 0.2	−3.9 ± 1.3	0.70 ± 0.09	58 ± 15	27 ± 3	49.4 ± 2.7	24.0 ± 3.1
		(n = 12)	(n = 3)	(n = 8)	(n = 8)	(n = 8)	(n = 12)	(n = 12)	(n = 12)
P2X4-pHluorin307	Head	185 ± 24	1.6 ± 0.3	−1.7 ± 0.8	0.54 ± 0.09	88 ± 20	36 ± 6	52.4 ± 4.1	11.6 ± 3.1
		(n = 12)	(n = 3)	(n = 8)	(n = 8)	(n = 9)	(n = 10)	(n = 10)	(n = 10)

Construct	Position region	Fluorescence intensity	GFP/β actin ratio	E_rev	Rectification index	I_ATP	Surface fraction	Acidic fraction	Other fluorescence
		a.u.		mV		−pA	%	%	%
WT P2X4	—	—	—	−0.1 ± 0.8	0.79 ± 0.08	2,351 ± 315	—	—	—
				(n = 14)	(n = 14)	(n = 17)
P2X4-YFP	C tail	738 ± 62	1.0 ± 0.05	—	—	—	—	—	—
		(n = 9)	(n = 3)
P2X4-pHluorin79	Head	163 ± 17	0.9 ± 0.1	ND	ND	24 ± 11	42 ± 2	41.2 ± 4.1	16.8 ± 2.3
		(n = 16)	(n = 3)			(n = 8)	(n = 11)	(n = 11)	(n = 11)
P2X4-pHluorin82	Head	188 ± 28	1.8 ± 0.03	2.4 ± 1.2	0.53 ± 0.03	587 ± 155	38 ± 3	52.2 ± 3.4	9.5 ± 1.9
		(n = 13)	(n = 3)	(n = 7)	(n = 7)	(n = 8)	(n = 11)	(n = 11)	(n = 11)
P2X4-pHluorin123	Beak	359 ± 25	2.0 ± 0.2	0.4 ± 0.7	0.67 ± 0.09	1,853 ± 271	38 ± 3	50.8 ± 3.1	11.2 ± 2.4
		(n = 14)	(n = 3)	(n = 9)	(n = 9)	(n = 10)	(n = 14)	(n = 14)	(n = 14)
P2X4-pHluorin156	Beak	343 ± 31	1.9 ± 0.2	−0.03 ± 0.90	0.47 ± 0.04	472 ± 81	28 ± 3	51.6 ± 3.2	20.4 ± 3.2
		(n = 16)	(n = 3)	(n = 7)	(n = 8)	(n = 9)	(n = 12)	(n = 12)	(n = 12)
P2X4-pHluorin207	Dorsal fin	240 ± 9	1.5 ± 0.1	ND	ND	0.4 ± 0.4	13 ± 2	33.5 ± 5.1	53.1 ± 5.6
		(n = 7)	(n = 3)			(n = 3)	(n = 6)	(n = 6)	(n = 6)
P2X4-pHluorin222	Dorsal fin	120 ± 10	0.9 ± 0.1	ND	ND	0.9 ± 1.1	22 ± 2	20.7 ± 5.2	57.5 ± 3.5
		(n = 6)	(n = 3)			(n = 5)	(n = 5)	(n = 5)	(n = 5)
P2X4-pHluorin224	Dorsal fin	199 ± 25	0.9 ± 0.1	ND	ND	5 ± 4	14 ± 5	36.0 ± 9.6	49.7 ± 9.5
		(n = 9)	(n = 3)			(n = 5)	(n = 6)	(n = 6)	(n = 6)
P2X4-pHluorin304	Head	177 ± 19	1.9 ± 0.2	−3.9 ± 1.3	0.70 ± 0.09	58 ± 15	27 ± 3	49.4 ± 2.7	24.0 ± 3.1
		(n = 12)	(n = 3)	(n = 8)	(n = 8)	(n = 8)	(n = 12)	(n = 12)	(n = 12)
P2X4-pHluorin307	Head	185 ± 24	1.6 ± 0.3	−1.7 ± 0.8	0.54 ± 0.09	88 ± 20	36 ± 6	52.4 ± 4.1	11.6 ± 3.1
		(n = 12)	(n = 3)	(n = 8)	(n = 8)	(n = 9)	(n = 10)	(n = 10)	(n = 10)

Fluorescence intensity documents the average fluorescence intensity of HEK-293 cells expressing the indicated constructs. The rectification index is the ratio of current + and −40 of the ATP-evoked current reversal potential (Erev). The surface, acidic, and nonacidic fractions of receptors were calculated as described in Materials and methods and Results.

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