Rate constants and parameters of the seven-state kinetic model
| Parameter | Value | SD |
| k120 (s−1) | 1,500 | 110 |
| k230 (s−1) | 59 | 2 |
| k340 (s−1) | 200 | 10 |
| k450 (M−2s−1) | 600,000 | 140,000 |
| k560 (M−1s−1) | 111,000 | 4,300 |
| k670 (s−1) | 300 | 25 |
| k710 (s−1) | 100 | 5 |
| zδ12 | -0.41 | 0.01 |
| zδ23 | 0.00 | 0.001 |
| zδ34 | −0.70 | 0.02 |
| zδ45 | −0.19 | 0.02 |
| zδ56 | −0.01 | 0.01 |
| zδ67 | −0.66 | 0.02 |
| zδ71 | −0.02 | 0.01 |
| k210 (s−1) | 200 | 35 |
| k320 (s−1) | 200 | 10 |
| k430 (s−1) | 1,900 | 55 |
| k540 (s−1) | 1,000 | 160 |
| k650 (s−1) | 82 | 5 |
| k760 (s−1) | 700 | 50 |
| k170 (M−3 s−1) | 6,700,000 | 220,000 |
| N90Na | 1.35 × 1011 | 3.6 × 109 |
| N50Na | 8.94 × 1010 | 2.5 × 109 |
| N10Na | 1.24 × 1011 | 5.5 × 109 |
| N0.1αMG | 7.34 × 1010 | 2.1 × 108 |
| N5αMG | 7.93 × 1010 | 6.3 × 108 |
| Parameter | Value | SD |
| k120 (s−1) | 1,500 | 110 |
| k230 (s−1) | 59 | 2 |
| k340 (s−1) | 200 | 10 |
| k450 (M−2s−1) | 600,000 | 140,000 |
| k560 (M−1s−1) | 111,000 | 4,300 |
| k670 (s−1) | 300 | 25 |
| k710 (s−1) | 100 | 5 |
| zδ12 | -0.41 | 0.01 |
| zδ23 | 0.00 | 0.001 |
| zδ34 | −0.70 | 0.02 |
| zδ45 | −0.19 | 0.02 |
| zδ56 | −0.01 | 0.01 |
| zδ67 | −0.66 | 0.02 |
| zδ71 | −0.02 | 0.01 |
| k210 (s−1) | 200 | 35 |
| k320 (s−1) | 200 | 10 |
| k430 (s−1) | 1,900 | 55 |
| k540 (s−1) | 1,000 | 160 |
| k650 (s−1) | 82 | 5 |
| k760 (s−1) | 700 | 50 |
| k170 (M−3 s−1) | 6,700,000 | 220,000 |
| N90Na | 1.35 × 1011 | 3.6 × 109 |
| N50Na | 8.94 × 1010 | 2.5 × 109 |
| N10Na | 1.24 × 1011 | 5.5 × 109 |
| N0.1αMG | 7.34 × 1010 | 2.1 × 108 |
| N5αMG | 7.93 × 1010 | 6.3 × 108 |
The seven-state model refers to the scheme presented in Fig. 3. The parameters highlighted in bold were obtained using the five-state model (absence of αMG) before expansion to the seven-state cotransport model. The αi’s (representing the energy barrier asymmetries) are fixed at 0.5. The rate constant k170 is adjusted to respect microreversibility. In the case of the five-state model, the SD was computed using 11 parameter sets reproducing satisfactorily the current traces averaged from four oocytes, whereas 8 parameter sets were used for the three additional transitions taking place in the seven-state model.