The aqueous diffusion coefficient of EGFP, Daq, EGFP, was reported previously to be 87 (Swaminathan et al., 1997; Brown et al., 1999; Potma et al., 2001) and 91 µm2 s−1 (Calvert et al., 2007). Daq, PAGFP was reported to be 89 µm2 s−1 (Calvert et al., 2007).