Calculated Concentrations of [Mg2+]free, Free [ATP], and [MgATP] in Solutions Used in Different Sets of Experiments
. | Mg2+total . | Mg2+free . | ATPtotal . | ATPfree . | Mg-ATP . |
|---|---|---|---|---|---|
| A | 0.00 | 0.00 | 3.00 | 3.00 | 0.00 |
| 1.53 | 0.10 | 3.00 | 1.58 | 1.42 | |
| 2.14 | 0.20 | 3.00 | 1.07 | 1.93 | |
| 2.97 | 0.50 | 3.00 | 0.54 | 2.46 | |
| 3.73 | 1.00 | 3.00 | 0.30 | 2.70 | |
| 5.99 | 3.00 | 3.00 | 0.11 | 2.89 | |
| B | 0.39 | 0.20 | 0.29 | 0.10 | 0.19 |
| 2.14 | 0.20 | 3.00 | 1.07 | 1.93 | |
| 5.61 | 0.20 | 8.40 | 3.00 | 5.40 | |
| C | 0.00 | 0.00 | 0.30 | 0.30 | 0.00 |
| 0.37 | 0.10 | 0.57 | 0.30 | 0.27 | |
| 0.75 | 0.20 | 0.84 | 0.30 | 0.54 | |
| 1.87 | 0.50 | 1.65 | 0.30 | 1.35 | |
| 3.73 | 1.00 | 3.00 | 0.30 | 2.70 | |
| 11.20 | 3.00 | 8.40 | 0.30 | 8.10 |
. | Mg2+total . | Mg2+free . | ATPtotal . | ATPfree . | Mg-ATP . |
|---|---|---|---|---|---|
| A | 0.00 | 0.00 | 3.00 | 3.00 | 0.00 |
| 1.53 | 0.10 | 3.00 | 1.58 | 1.42 | |
| 2.14 | 0.20 | 3.00 | 1.07 | 1.93 | |
| 2.97 | 0.50 | 3.00 | 0.54 | 2.46 | |
| 3.73 | 1.00 | 3.00 | 0.30 | 2.70 | |
| 5.99 | 3.00 | 3.00 | 0.11 | 2.89 | |
| B | 0.39 | 0.20 | 0.29 | 0.10 | 0.19 |
| 2.14 | 0.20 | 3.00 | 1.07 | 1.93 | |
| 5.61 | 0.20 | 8.40 | 3.00 | 5.40 | |
| C | 0.00 | 0.00 | 0.30 | 0.30 | 0.00 |
| 0.37 | 0.10 | 0.57 | 0.30 | 0.27 | |
| 0.75 | 0.20 | 0.84 | 0.30 | 0.54 | |
| 1.87 | 0.50 | 1.65 | 0.30 | 1.35 | |
| 3.73 | 1.00 | 3.00 | 0.30 | 2.70 | |
| 11.20 | 3.00 | 8.40 | 0.30 | 8.10 |
(A) In this set of solutions, total ATP remains constant, but [Mg2+]free and [ATP]free both vary. (B) [Mg2+]free is kept constant. (C) [Mg2+]free is variable but [ATP]free is constant. Values representing variable [Mg2+]free or [ATP]free are shown in bold for clarity.