Table 1.

Antibodies and primers

Antibodies and primersSourceIdentifier
Antibodies 
Anti-mouse TREX1 antibody BD Biosciences Cat #611986 
Anti-human TREX1 antibody Cell Signaling Technology Cat #15107S 
Anti-MLH1 antibody Abcam Cat #ab92312 
β-Actin (13E5) Rabbit mAb, Anti-mouse IgG, HRP-linked antibody, Anti-rabbit IgG, HRP-linked antibody Cell Signaling Technology Cat #4970, Cat #7076, Cat #7074 
PE anti-mouse H-2Kd/H-2Dd antibody BioLegend Cat #114708 
PE anti-mouse H-2Kb antibody BioLegend Cat # 116508 
PE anti-mouse CD3ε antibody BioLegend Cat #155608 
PE/Cyanine7 anti-mouse CD45.2 antibody BioLegend Cat #109830 
PerCP anti-mouse CD4 antibody BioLegend Cat #100432 
Alexa Fluor 700 anti-mouse CD8a antibody BioLegend Cat #100730 
APC anti-mouse CD49b antibody BioLegend Cat #103516 
FITC anti-mouse I-A/I-E antibody BioLegend Cat #107606 
Pacific Blue anti-mouse CD86 antibody BioLegend Cat #105022 
APC anti-mouse CD80 antibody BioLegend Cat #104714 
PE/Cyanine7 anti-mouse CD366 (Tim-3) antibody BioLegend Cat #134010 
Brilliant Violet 510 anti-mouse CD279 (PD-1) antibody BioLegend Cat #135241 
Brilliant Violet 510 anti-mouse CD11c antibody BioLegend Cat #124623 
eBioscience Fixable Viability Dye eFluor 780 Thermo Fisher Scientific Cat #65-0865-14 
Primers 
Mouse DFFB forward: 5′-GGT​TAC​CTA​AGA​GAG​GTG​AGC​G-3′ This paper N/A 
Mouse DFFB reverse: 5′-ACC​ACG​GTG​CGC​TTC​TTC-3′ This paper N/A 
Mouse DNAse1L1 forward: 5′-GCG​CAG​CAC​ATA​CAA​GGA​AA-3′ This paper N/A 
Mouse DNAse1L1 reverse: 5′-TGA​TGC​GGA​GAG​CCT​CAT​TC-3′ This paper N/A 
Mouse DNAse1 forward: 5′-
AGGCCTGACCAGGTGTCTAT-3′ 		This paper N/A 
Mouse DNAse1 reverse: 5′-AAG​ATG​GGG​CTT​GTC​CGA​AG-3′ This paper N/A 
Mouse DNAse1L2 forward: 5′-GCA​GTG​TCA​TAG​CAC​AGA​TCC-3′ This paper N/A 
Mouse DNAse1L2 reverse: 5′-TTG​GTG​GCA​CAG​CTA​GGA​AC-3′ This paper N/A 
Mouse DNAse1L3 forward: 5′-TGA​TTG​TCC​CCT​TGC​ACA​CA-3′ This paper N/A 
Mouse DNAse1L3 reverse: 5′-GTC​CAC​AAA​GCA​CAA​TCC​TGT​C-3′ This paper N/A 
Mouse DNAse2a forward: 5′-TGG​GCA​TAC​GAA​GGG​CAA​G-3′ This paper N/A 
Mouse DNAse2a reverse: 5′-TAG​GCT​CCA​GAG​CAG​TTG​GA-3′ This paper N/A 
Mouse DNAse2b forward: 5′-CCA​CTC​GCA​GGA​AGT​GAA​C-3′ This paper N/A 
Mouse DNAse2b reverse: 5′-CTT​GTG​GAG​TCC​AGG​TAC​AGG-3′ This paper N/A 
Mouse IFNb forward: 5′-ATG​AGT​GGT​GGT​TGC​AGG​C-3′ This paper N/A 
Mouse IFNb reverse: 5′-TGA​CCT​TTC​AAA​TGC​AGT​AGA​TTC​A-3′ This paper N/A 
Mouse ISG15 forward: 5′-CAT​CCT​GGT​GAG​GAA​CGA​AAG​G-3′ This paper N/A 
Mouse ISG15 reverse: 5′-CTC​AGC​CAG​AAC​TGG​TCT​TCG​T-3′ This paper N/A 
Mouse CXCL10 forward: 5′-GGA​GTG​AAG​CCA​CGC​ACA​C-3′ This paper N/A 
Mouse CXCL10 reverse: 5′-ATG​GAG​AGA​GGC​TCT​CT GCTGT-3′ This paper N/A 
Mouse TREX1 forward: 5′-AGC​ATC​TGT​CAG​TGG​AAG​CC-3′ This paper N/A 
Mouse TREX1 reverse: 5′-TGG​CTA​TTG​CCA​AGG​TCA​GG-3′ This paper N/A 
Mouse GAPDH forward: 5′-CAT​CAA​GAA​GGT​GGT​GAA​GC-3′ This paper N/A 
Mouse GAPDH reverse: 5′-CCT​GTT​GCT​GTA​GCC​GTA​TT-3′ This paper N/A 
Mouse IRF1 forward: 5′-CTT​CGT​CGA​GGT​AGG​ACG​TG-3′ This paper N/A 
Mouse IRF1 reverse: 5′-TGC​ATC​TCT​AGC​CAG​GGT​CT-3′ This paper N/A 
Mouse 18s rRNA forward: 5′-GTA​ACC​CGT​TGA​ACC​CCA​TT-3′ This paper N/A 
Mouse 18s rRNA reverse: 5′-CCA​TCC​AAT​CGG​TAG​TAG​CG-3′ This paper N/A 
Human 18s rRNA forward: 5′-ACC​GAT​TGG​ATG​GTT​TAG​TGA​G-3′ This paper N/A 
Human 18s rRNA reverse: 5′-CCT​ACG​GAA​ACC​TTG​TTA​CGA​C-3′ This paper N/A 
Human IFNb forward: 5′-CAA​CTT​GCT​TGG​ATT​CCT​ACA​AAG-3′ This paper N/A 
Human IFNb reverse: 5′-TAT​TCA​AGC​CTC​CCA​TTC​AAT​TG-3′ This paper N/A 
sgTREX1: 5′-AGTGCCCAGA CCGCCCCGTG-3′ This paper N/A 
sgSTING: 5′-CAC​CTA​GCC​TCG​CAC​GAA​CT-3′ This paper N/A 
sgIFNAR1: 5′-GTT​GTA​GTA​TGT​TGA​CAT​TC-3′ This paper N/A 
sgMLH1: 5′-TAA​TAG​TGA​CAT​GGG​CCA​CA-3′ This paper N/A 
sgcGAS: 5′-CGC​AAA​GGG​GGG​CTC​GAT​CG-3′ This paper N/A 
sgTBK1: 5′-CAC​AGG​CCG​GAC​GTC​CCC​AG-3′ This paper N/A 
shMLH1: 5′-GTG​TTC​TTC​TTT​CTC​TGT​ATT-3′ This paper N/A 
Antibodies and primersSourceIdentifier
Antibodies 
Anti-mouse TREX1 antibody BD Biosciences Cat #611986 
Anti-human TREX1 antibody Cell Signaling Technology Cat #15107S 
Anti-MLH1 antibody Abcam Cat #ab92312 
β-Actin (13E5) Rabbit mAb, Anti-mouse IgG, HRP-linked antibody, Anti-rabbit IgG, HRP-linked antibody Cell Signaling Technology Cat #4970, Cat #7076, Cat #7074 
PE anti-mouse H-2Kd/H-2Dd antibody BioLegend Cat #114708 
PE anti-mouse H-2Kb antibody BioLegend Cat # 116508 
PE anti-mouse CD3ε antibody BioLegend Cat #155608 
PE/Cyanine7 anti-mouse CD45.2 antibody BioLegend Cat #109830 
PerCP anti-mouse CD4 antibody BioLegend Cat #100432 
Alexa Fluor 700 anti-mouse CD8a antibody BioLegend Cat #100730 
APC anti-mouse CD49b antibody BioLegend Cat #103516 
FITC anti-mouse I-A/I-E antibody BioLegend Cat #107606 
Pacific Blue anti-mouse CD86 antibody BioLegend Cat #105022 
APC anti-mouse CD80 antibody BioLegend Cat #104714 
PE/Cyanine7 anti-mouse CD366 (Tim-3) antibody BioLegend Cat #134010 
Brilliant Violet 510 anti-mouse CD279 (PD-1) antibody BioLegend Cat #135241 
Brilliant Violet 510 anti-mouse CD11c antibody BioLegend Cat #124623 
eBioscience Fixable Viability Dye eFluor 780 Thermo Fisher Scientific Cat #65-0865-14 
Primers 
Mouse DFFB forward: 5′-GGT​TAC​CTA​AGA​GAG​GTG​AGC​G-3′ This paper N/A 
Mouse DFFB reverse: 5′-ACC​ACG​GTG​CGC​TTC​TTC-3′ This paper N/A 
Mouse DNAse1L1 forward: 5′-GCG​CAG​CAC​ATA​CAA​GGA​AA-3′ This paper N/A 
Mouse DNAse1L1 reverse: 5′-TGA​TGC​GGA​GAG​CCT​CAT​TC-3′ This paper N/A 
Mouse DNAse1 forward: 5′-
AGGCCTGACCAGGTGTCTAT-3′ 		This paper N/A 
Mouse DNAse1 reverse: 5′-AAG​ATG​GGG​CTT​GTC​CGA​AG-3′ This paper N/A 
Mouse DNAse1L2 forward: 5′-GCA​GTG​TCA​TAG​CAC​AGA​TCC-3′ This paper N/A 
Mouse DNAse1L2 reverse: 5′-TTG​GTG​GCA​CAG​CTA​GGA​AC-3′ This paper N/A 
Mouse DNAse1L3 forward: 5′-TGA​TTG​TCC​CCT​TGC​ACA​CA-3′ This paper N/A 
Mouse DNAse1L3 reverse: 5′-GTC​CAC​AAA​GCA​CAA​TCC​TGT​C-3′ This paper N/A 
Mouse DNAse2a forward: 5′-TGG​GCA​TAC​GAA​GGG​CAA​G-3′ This paper N/A 
Mouse DNAse2a reverse: 5′-TAG​GCT​CCA​GAG​CAG​TTG​GA-3′ This paper N/A 
Mouse DNAse2b forward: 5′-CCA​CTC​GCA​GGA​AGT​GAA​C-3′ This paper N/A 
Mouse DNAse2b reverse: 5′-CTT​GTG​GAG​TCC​AGG​TAC​AGG-3′ This paper N/A 
Mouse IFNb forward: 5′-ATG​AGT​GGT​GGT​TGC​AGG​C-3′ This paper N/A 
Mouse IFNb reverse: 5′-TGA​CCT​TTC​AAA​TGC​AGT​AGA​TTC​A-3′ This paper N/A 
Mouse ISG15 forward: 5′-CAT​CCT​GGT​GAG​GAA​CGA​AAG​G-3′ This paper N/A 
Mouse ISG15 reverse: 5′-CTC​AGC​CAG​AAC​TGG​TCT​TCG​T-3′ This paper N/A 
Mouse CXCL10 forward: 5′-GGA​GTG​AAG​CCA​CGC​ACA​C-3′ This paper N/A 
Mouse CXCL10 reverse: 5′-ATG​GAG​AGA​GGC​TCT​CT GCTGT-3′ This paper N/A 
Mouse TREX1 forward: 5′-AGC​ATC​TGT​CAG​TGG​AAG​CC-3′ This paper N/A 
Mouse TREX1 reverse: 5′-TGG​CTA​TTG​CCA​AGG​TCA​GG-3′ This paper N/A 
Mouse GAPDH forward: 5′-CAT​CAA​GAA​GGT​GGT​GAA​GC-3′ This paper N/A 
Mouse GAPDH reverse: 5′-CCT​GTT​GCT​GTA​GCC​GTA​TT-3′ This paper N/A 
Mouse IRF1 forward: 5′-CTT​CGT​CGA​GGT​AGG​ACG​TG-3′ This paper N/A 
Mouse IRF1 reverse: 5′-TGC​ATC​TCT​AGC​CAG​GGT​CT-3′ This paper N/A 
Mouse 18s rRNA forward: 5′-GTA​ACC​CGT​TGA​ACC​CCA​TT-3′ This paper N/A 
Mouse 18s rRNA reverse: 5′-CCA​TCC​AAT​CGG​TAG​TAG​CG-3′ This paper N/A 
Human 18s rRNA forward: 5′-ACC​GAT​TGG​ATG​GTT​TAG​TGA​G-3′ This paper N/A 
Human 18s rRNA reverse: 5′-CCT​ACG​GAA​ACC​TTG​TTA​CGA​C-3′ This paper N/A 
Human IFNb forward: 5′-CAA​CTT​GCT​TGG​ATT​CCT​ACA​AAG-3′ This paper N/A 
Human IFNb reverse: 5′-TAT​TCA​AGC​CTC​CCA​TTC​AAT​TG-3′ This paper N/A 
sgTREX1: 5′-AGTGCCCAGA CCGCCCCGTG-3′ This paper N/A 
sgSTING: 5′-CAC​CTA​GCC​TCG​CAC​GAA​CT-3′ This paper N/A 
sgIFNAR1: 5′-GTT​GTA​GTA​TGT​TGA​CAT​TC-3′ This paper N/A 
sgMLH1: 5′-TAA​TAG​TGA​CAT​GGG​CCA​CA-3′ This paper N/A 
sgcGAS: 5′-CGC​AAA​GGG​GGG​CTC​GAT​CG-3′ This paper N/A 
sgTBK1: 5′-CAC​AGG​CCG​GAC​GTC​CCC​AG-3′ This paper N/A 
shMLH1: 5′-GTG​TTC​TTC​TTT​CTC​TGT​ATT-3′ This paper N/A 
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