Table 2.

Available published data on TREC-based NBS including number of identified patients with SCID and AT

Number of screened newbornsNumber of identified SCIDNumber of early identified ATTREC levels, (patient; cutoff)Other notable (clinical) findingsReference(s)
3,252,156 50 5/5 abnormal (4–18; cutoff copies per µl) 5/213 (2.4%) abnormal screening results were AT
Initial T cell counts of 800–1,323 cells per μL
1 patient presented with low IgG and IgA, normal IgM, and received Ig-RT 
(19) (USA, California)
(14) 
670,580 2/2 abnormal (cutoff and TREC levels NA) 2/68 (3%) abnormal screening results were AT
Initial CD3 counts were 1,474 and 945 cells per μL 
(37) (USA, Wisconsin) 
∼700,000 4/4 abnormal (7–13; cutoff 25 copies per µl) 4/63 (6.4%) of all abnormal screening results were AT
2/4 patients with AT showed increased IgM with decreased total IgG and IgA
3/4 patients with AT showed decreased T cell stimulation with PHA
Diminished vaccination responses in AT 
(15) (Canada) 
58,834 Abnormal (5; cutoff copies per 3.2 mm punch) 1/64 (1.6%) recalls for secondary testing resulted in AT
KRECs also below cutoff Patient asymptomatic at 1 year, but with IgA deficiency 
(40) (Sweden) 
1,900,00 25 SCID, 17 leaky SCID/Omenn syndrome Abnormal (cutoff and TREC levels NA) 1/88 (1.1%) of total abnormal screening results was AT
1/46 (2.17%) of syndromic causes of low TRECs was AT
Patient underwent successful, uneventful HSCT procedure at age 8 mo (alive and well at 18 mo of age) 
(39) (Germany) 
NA NA Abnormal (65; cutoff 200 copies per uL) Low T cells, low IgG and IgA with normal IgM. Hospital admission for infection, broad-spectrum antibiotics, Ig-replacement therapy, and antibiotic prophylaxis (38) (USA, New York) 
NA NA Abnormal (8; cutoff 10 copies per 3.2 mm punch) Low T cells with Pneumocystis jiroveci Pneumonia (PJP) prophylaxis (cotrimoxazol), HIGM, and decreased IgA that spontaneously improved (16) (Netherlands) 
Number of screened newbornsNumber of identified SCIDNumber of early identified ATTREC levels, (patient; cutoff)Other notable (clinical) findingsReference(s)
3,252,156 50 5/5 abnormal (4–18; cutoff copies per µl) 5/213 (2.4%) abnormal screening results were AT
Initial T cell counts of 800–1,323 cells per μL
1 patient presented with low IgG and IgA, normal IgM, and received Ig-RT 
(19) (USA, California)
(14) 
670,580 2/2 abnormal (cutoff and TREC levels NA) 2/68 (3%) abnormal screening results were AT
Initial CD3 counts were 1,474 and 945 cells per μL 
(37) (USA, Wisconsin) 
∼700,000 4/4 abnormal (7–13; cutoff 25 copies per µl) 4/63 (6.4%) of all abnormal screening results were AT
2/4 patients with AT showed increased IgM with decreased total IgG and IgA
3/4 patients with AT showed decreased T cell stimulation with PHA
Diminished vaccination responses in AT 
(15) (Canada) 
58,834 Abnormal (5; cutoff copies per 3.2 mm punch) 1/64 (1.6%) recalls for secondary testing resulted in AT
KRECs also below cutoff Patient asymptomatic at 1 year, but with IgA deficiency 
(40) (Sweden) 
1,900,00 25 SCID, 17 leaky SCID/Omenn syndrome Abnormal (cutoff and TREC levels NA) 1/88 (1.1%) of total abnormal screening results was AT
1/46 (2.17%) of syndromic causes of low TRECs was AT
Patient underwent successful, uneventful HSCT procedure at age 8 mo (alive and well at 18 mo of age) 
(39) (Germany) 
NA NA Abnormal (65; cutoff 200 copies per uL) Low T cells, low IgG and IgA with normal IgM. Hospital admission for infection, broad-spectrum antibiotics, Ig-replacement therapy, and antibiotic prophylaxis (38) (USA, New York) 
NA NA Abnormal (8; cutoff 10 copies per 3.2 mm punch) Low T cells with Pneumocystis jiroveci Pneumonia (PJP) prophylaxis (cotrimoxazol), HIGM, and decreased IgA that spontaneously improved (16) (Netherlands) 

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