Plasmids used in this study
| Shorthand name . | Sequence . | Reference . |
|---|---|---|
| NES-PABD-Spo20 | X. laevis map2k1.L32-44-APVAT-EGFP-GLRSRASI-S. cerevisiae Spo20p51-91 | Zeniou-Meyer et al. (2007), Zhang et al. (2014) |
| PASS | EGFP-SGLRSRA-M. musculus PKIa34-51-SR-S. cerevisiae Spo20p51-91 | Zhang et al. (2014), Zeniou-Meyer et al. (2007) |
| NES-flex-PABD-Spo20 | X. laevis map2k1.L32-44-APVAT-EGFP-SGGGSGGS-S. cerevisiae Spo20p51-91 | This study |
| NES-PABDx2-Spo20 | X. laevis map2k1.L32-44-APVAT-EGFP-SGLRSRA-S. cerevisiae Spo20p51-91- S. cerevisiae Spo20p51-91 | Bohdanowicz et al. (2013) |
| NESx2-PABDx2-Spo20 | X. laevis map2k1.L32-44-AGGSG-X. laevis map2k1.L32-44-APVAT-EGFP-SGLRSRA-S. cerevisiae Spo20p51-91- S. cerevisiae Spo20p51-91 | This study |
| PILS-Nir1 (Nir1-LNS2) | NeonGreen-GGSGGM-(PITPNM3) Nir1613-897 | This study |
| Nir2-LNS2 | NeonGreen-GGSGGM-(PITPNM1) Nir2896-1216 | This study |
| Nir3-LNS2 | pcDNA3.1-mEGFP-GGGGSHM-(PITPNM2 isoform 6) Nir3925-1209 | This study |
| PILS-Nir1-613-630 | (PITPNM3) Nir1613-630-GGSGG-NeonGreen | This study |
| PILS-Nir1-631-894 | NeonGreen-GGSGG-(PITPNM3) Nir1631-894 | This study |
| PILS-Nir1-LNS2-K820E | NeonGreen-GGSGGM-(PITPNM3) Nir1613-897, K820E | This study |
| C1ab-Prkd1 | X. laevis map2k1.L32-44-APVAT-mCherry-SGLRSRAQASNSTS-M. musculus Prkd1138-343 | Kim et al. (2011) |
| FKBP-PJ-dead | mCherry-SGLRSRSAAAGAGGAARAALG-FKBP1A3-109-SAGGSAGGSAGGSAGGSAGGPRAQASRSLDA-S. cerevisiae Sac12-517, C392S -GGTARGAAAGAGGAGR-INPP5E214-644, D556A,C641A | Hammond et al. (2012) |
| FKBP-PJ | mCherry-SGLRSRSAAAGAGGAARAALG-FKBP1A3-109-SAGGSAGGSAGGSAGGSAGGPRAQASRSLDA-S. cerevisiae Sac12-517-GGTARGAAAGAGGAGR-INPP5E214-644, C641A | Hammond et al. (2012) |
| FKBP-INPP5E | mCherry-SGLRSRSAAAGAGGAARAAMG-FKBP1A3-109-ARGAAAGAGGAGR-INPP5E214-644, C641A | Hammond et al. (2012) |
| FKBP-PJ-Sac1 | mCherry-SGLRSRSAAAGAGGAARAALG-FKBP1A3-109-SAGGSAGGSAGGSAGGSAGGPRAQASRSLDA-S. cerevisiae Sac12-517-GGTARGAAAGAGGAGR-INPP5E214-644, D556A C641A | Hammond et al. (2012) |
| BFP-Tubby(C) | M. musculus Tubby protein243-505-TVPRARDA-pTagBFP-KRPRL | Quinn et al. (2008) |
| P4Mx1 | X. laevis map2k1.L32-44-APVAT-mTagBFP2-SGLRSRAQASNSAVDGGSASGLRS- L. penomophila SidM546-647 | Zewe et al. (2020) |
| PM-FRB | Lyn1-11-RSANSGAGAGAGAILSR-MTOR2021-2113-TSYPYDVPDYAPVAT-iRFP | Hammond et al. (2014) |
| Mito-FRB | iRFP-SGLRSRAGGAGAILSR-MTOR2021-2113-GGSAGGSAQASNSAVDGTA-Fis1122-152 | Doyle et al. (2024), Preprint |
| FKBP-PI4K | mCherry-SGLRSRSAAAGAGGAARAAL-FKBP1A3-108-SAGGSAGGSAGGSAGGSAGGPRAQASNSL-PI4KA1102-2103 | Zewe et al. (2020) |
| FKBP-PIP5K | pTagBFP-SGLRSRSAAAGAGGAARAALG-FKBP1A3-108-SAGGSAGGSAGGSAGGSAGGPRAQASNSAVDLQA-PIP5K1C1-640,D101R, R304D, R445E, K446E | This study |
| EGFP-PH-PLCδ1 | PLCδ11-170-DPPVAT-EGFP | Várnai and Balla (1998) |
| FKBP-PI-PLC | B. cereus PI-PLC32-329, W78A, W273A-RILQSTVPMG-FKBP1A3-108-RDPPVATM-TagBFP2-SGLRSRSAAATLDHNQPYHICRGFTCFKKPPTPPPEPET | Pemberton et al. (2020) |
| FKBP-DGKa | mRFP-SGLRSRSAAAGAGGAARAAL-FKBP1A3-108-SAGGSAGGSAGGSAGGSAGGPRAQASRS-DGKA isoform b394-773 | This study |
| M3 | pcDNA3.1-HAx3-AchR-CHRM32-590 | J. Wess |
| ER-FRB | iRFP713- SGLRSRAQLTMAYPYDVPDYVA-MTOR2021-2113- QGSGAGAGAGAILNSRV-SACM1L418-484 | Zewe et al. (2020) |
| Golgi-FRB | iRFP-SGLRSRAGGAGAILSR-MTOR2021-2113-GGSAGGSA-GOLGB13096-3224 | Zewe et al. (2020) |
| Rab5-FRB | iRFP713-SGLRSRAGGAGAILSR-MTOR2021-2113-GGSAGGSAQASNSAVDGT-C. lupus Rab5a1-215 | Hammond et al. (2012) |
| GFP-Nir2 | EGFP-SGLRSRAQASNS-PITPNM1v2 | Kim et al. (2015) |
| iRFP-PILS-Nir1 | miRFP670-GGSGGM-(PITPNM3) Nir1613-897 | This study |
| iRFP-Tubby(c) | M. musculus Tubby243-505-PRARDPPVAT-miRFP670 | Quinn et al. (2008) |
| iRFP-PH-PLCδ1 | iRFP713-CTRDLELKL-R. norvegicus PLCD1 isoform X32-131 | Idevall-Hagren et al. (2012) |
| TagBFP2-HRAS-CAAX | TagBFP2-SGLRSRAQASNSAVD-HRAS172-189 | Goulden et al. (2019) |
| mCh-HRAS-CAAX | mCherry-SGLRSRAQASNSAVD-HRAS172-189 | This study |
| pUC19 | Empty plasmid used to bring total DNA mass up to 1 μg as needed | Yanisch-Perron et al. (1985) |
| Shorthand name . | Sequence . | Reference . |
|---|---|---|
| NES-PABD-Spo20 | X. laevis map2k1.L32-44-APVAT-EGFP-GLRSRASI-S. cerevisiae Spo20p51-91 | Zeniou-Meyer et al. (2007), Zhang et al. (2014) |
| PASS | EGFP-SGLRSRA-M. musculus PKIa34-51-SR-S. cerevisiae Spo20p51-91 | Zhang et al. (2014), Zeniou-Meyer et al. (2007) |
| NES-flex-PABD-Spo20 | X. laevis map2k1.L32-44-APVAT-EGFP-SGGGSGGS-S. cerevisiae Spo20p51-91 | This study |
| NES-PABDx2-Spo20 | X. laevis map2k1.L32-44-APVAT-EGFP-SGLRSRA-S. cerevisiae Spo20p51-91- S. cerevisiae Spo20p51-91 | Bohdanowicz et al. (2013) |
| NESx2-PABDx2-Spo20 | X. laevis map2k1.L32-44-AGGSG-X. laevis map2k1.L32-44-APVAT-EGFP-SGLRSRA-S. cerevisiae Spo20p51-91- S. cerevisiae Spo20p51-91 | This study |
| PILS-Nir1 (Nir1-LNS2) | NeonGreen-GGSGGM-(PITPNM3) Nir1613-897 | This study |
| Nir2-LNS2 | NeonGreen-GGSGGM-(PITPNM1) Nir2896-1216 | This study |
| Nir3-LNS2 | pcDNA3.1-mEGFP-GGGGSHM-(PITPNM2 isoform 6) Nir3925-1209 | This study |
| PILS-Nir1-613-630 | (PITPNM3) Nir1613-630-GGSGG-NeonGreen | This study |
| PILS-Nir1-631-894 | NeonGreen-GGSGG-(PITPNM3) Nir1631-894 | This study |
| PILS-Nir1-LNS2-K820E | NeonGreen-GGSGGM-(PITPNM3) Nir1613-897, K820E | This study |
| C1ab-Prkd1 | X. laevis map2k1.L32-44-APVAT-mCherry-SGLRSRAQASNSTS-M. musculus Prkd1138-343 | Kim et al. (2011) |
| FKBP-PJ-dead | mCherry-SGLRSRSAAAGAGGAARAALG-FKBP1A3-109-SAGGSAGGSAGGSAGGSAGGPRAQASRSLDA-S. cerevisiae Sac12-517, C392S -GGTARGAAAGAGGAGR-INPP5E214-644, D556A,C641A | Hammond et al. (2012) |
| FKBP-PJ | mCherry-SGLRSRSAAAGAGGAARAALG-FKBP1A3-109-SAGGSAGGSAGGSAGGSAGGPRAQASRSLDA-S. cerevisiae Sac12-517-GGTARGAAAGAGGAGR-INPP5E214-644, C641A | Hammond et al. (2012) |
| FKBP-INPP5E | mCherry-SGLRSRSAAAGAGGAARAAMG-FKBP1A3-109-ARGAAAGAGGAGR-INPP5E214-644, C641A | Hammond et al. (2012) |
| FKBP-PJ-Sac1 | mCherry-SGLRSRSAAAGAGGAARAALG-FKBP1A3-109-SAGGSAGGSAGGSAGGSAGGPRAQASRSLDA-S. cerevisiae Sac12-517-GGTARGAAAGAGGAGR-INPP5E214-644, D556A C641A | Hammond et al. (2012) |
| BFP-Tubby(C) | M. musculus Tubby protein243-505-TVPRARDA-pTagBFP-KRPRL | Quinn et al. (2008) |
| P4Mx1 | X. laevis map2k1.L32-44-APVAT-mTagBFP2-SGLRSRAQASNSAVDGGSASGLRS- L. penomophila SidM546-647 | Zewe et al. (2020) |
| PM-FRB | Lyn1-11-RSANSGAGAGAGAILSR-MTOR2021-2113-TSYPYDVPDYAPVAT-iRFP | Hammond et al. (2014) |
| Mito-FRB | iRFP-SGLRSRAGGAGAILSR-MTOR2021-2113-GGSAGGSAQASNSAVDGTA-Fis1122-152 | Doyle et al. (2024), Preprint |
| FKBP-PI4K | mCherry-SGLRSRSAAAGAGGAARAAL-FKBP1A3-108-SAGGSAGGSAGGSAGGSAGGPRAQASNSL-PI4KA1102-2103 | Zewe et al. (2020) |
| FKBP-PIP5K | pTagBFP-SGLRSRSAAAGAGGAARAALG-FKBP1A3-108-SAGGSAGGSAGGSAGGSAGGPRAQASNSAVDLQA-PIP5K1C1-640,D101R, R304D, R445E, K446E | This study |
| EGFP-PH-PLCδ1 | PLCδ11-170-DPPVAT-EGFP | Várnai and Balla (1998) |
| FKBP-PI-PLC | B. cereus PI-PLC32-329, W78A, W273A-RILQSTVPMG-FKBP1A3-108-RDPPVATM-TagBFP2-SGLRSRSAAATLDHNQPYHICRGFTCFKKPPTPPPEPET | Pemberton et al. (2020) |
| FKBP-DGKa | mRFP-SGLRSRSAAAGAGGAARAAL-FKBP1A3-108-SAGGSAGGSAGGSAGGSAGGPRAQASRS-DGKA isoform b394-773 | This study |
| M3 | pcDNA3.1-HAx3-AchR-CHRM32-590 | J. Wess |
| ER-FRB | iRFP713- SGLRSRAQLTMAYPYDVPDYVA-MTOR2021-2113- QGSGAGAGAGAILNSRV-SACM1L418-484 | Zewe et al. (2020) |
| Golgi-FRB | iRFP-SGLRSRAGGAGAILSR-MTOR2021-2113-GGSAGGSA-GOLGB13096-3224 | Zewe et al. (2020) |
| Rab5-FRB | iRFP713-SGLRSRAGGAGAILSR-MTOR2021-2113-GGSAGGSAQASNSAVDGT-C. lupus Rab5a1-215 | Hammond et al. (2012) |
| GFP-Nir2 | EGFP-SGLRSRAQASNS-PITPNM1v2 | Kim et al. (2015) |
| iRFP-PILS-Nir1 | miRFP670-GGSGGM-(PITPNM3) Nir1613-897 | This study |
| iRFP-Tubby(c) | M. musculus Tubby243-505-PRARDPPVAT-miRFP670 | Quinn et al. (2008) |
| iRFP-PH-PLCδ1 | iRFP713-CTRDLELKL-R. norvegicus PLCD1 isoform X32-131 | Idevall-Hagren et al. (2012) |
| TagBFP2-HRAS-CAAX | TagBFP2-SGLRSRAQASNSAVD-HRAS172-189 | Goulden et al. (2019) |
| mCh-HRAS-CAAX | mCherry-SGLRSRAQASNSAVD-HRAS172-189 | This study |
| pUC19 | Empty plasmid used to bring total DNA mass up to 1 μg as needed | Yanisch-Perron et al. (1985) |
All genes are Homo sapiens unless indicated otherwise. The relevant amino acid positions in the full-length protein are noted. Mutations are described by the position of the residues in the full-length protein. Amino acid sequences written out indicate linkers.