Reagents used for cell stimulation throughout this study
| Reagent . | Manufacturer . | Catalog number . | Stock solution . | Storage temperature . | Concentration added to cells (diluted in cell media) . |
|---|---|---|---|---|---|
| PMA | MilliporeSigma | P8139 | 437 µM in DMSO | −20°C | 100 nM |
| FIPI | MilliporeSigma | 528245 | 750 µM in DMSO | −20°C | 750 nM |
| DiC8 | EMD Millipore | 317505 | 30 mM in methanol, dried. Resuspended in 50 μl methanol before use | −80°C | 150 µM |
| OAG | EMD Millipore | 495414 | 30 mM in methanol, dried. Resuspended in 20 μl DMSO before use | −80°C | 150 µM |
| PDBu | Sigma-Aldrich | P1269 | 10 mM in DMSO | −80°C | 5 µM |
| Rapamycin | Sigma-Aldrich | 553210 | 1 mM in DMSO | −20°C | 1 µM |
| CCh | Thermo Fisher Scientific | AC10824 | 50 mM in dH2O | −20°C | 5 µM (Fig. 7), 10 M (Fig. 5), or 100 µM (Fig. 6) |
| Atropine | Sigma-Aldrich | A0257 | 25 mM in dH2O | 4°C | 5 µM |
| ATP | Sigma-Aldrich | 10127523001 | 100 mM in 100 mM MgCl2 + 200 mM Tris base | −20°C | 100 µM |
| Histamine | Thermo Fisher Scientific | AC15062 | 100 mM in dH2O | −20°C | 100 µM |
| Reagent . | Manufacturer . | Catalog number . | Stock solution . | Storage temperature . | Concentration added to cells (diluted in cell media) . |
|---|---|---|---|---|---|
| PMA | MilliporeSigma | P8139 | 437 µM in DMSO | −20°C | 100 nM |
| FIPI | MilliporeSigma | 528245 | 750 µM in DMSO | −20°C | 750 nM |
| DiC8 | EMD Millipore | 317505 | 30 mM in methanol, dried. Resuspended in 50 μl methanol before use | −80°C | 150 µM |
| OAG | EMD Millipore | 495414 | 30 mM in methanol, dried. Resuspended in 20 μl DMSO before use | −80°C | 150 µM |
| PDBu | Sigma-Aldrich | P1269 | 10 mM in DMSO | −80°C | 5 µM |
| Rapamycin | Sigma-Aldrich | 553210 | 1 mM in DMSO | −20°C | 1 µM |
| CCh | Thermo Fisher Scientific | AC10824 | 50 mM in dH2O | −20°C | 5 µM (Fig. 7), 10 M (Fig. 5), or 100 µM (Fig. 6) |
| Atropine | Sigma-Aldrich | A0257 | 25 mM in dH2O | 4°C | 5 µM |
| ATP | Sigma-Aldrich | 10127523001 | 100 mM in 100 mM MgCl2 + 200 mM Tris base | −20°C | 100 µM |
| Histamine | Thermo Fisher Scientific | AC15062 | 100 mM in dH2O | −20°C | 100 µM |