Table 1.

Reagents used for cell stimulation throughout this study

ReagentManufacturerCatalog numberStock solutionStorage temperatureConcentration added to cells (diluted in cell media)
PMA MilliporeSigma P8139 437 µM in DMSO −20°C 100 nM 
FIPI MilliporeSigma 528245 750 µM in DMSO −20°C 750 nM 
DiC8 EMD Millipore 317505 30 mM in methanol, dried. Resuspended in 50 μl methanol before use −80°C 150 µM 
OAG EMD Millipore 495414 30 mM in methanol, dried. Resuspended in 20 μl DMSO before use −80°C 150 µM 
PDBu Sigma-Aldrich P1269 10 mM in DMSO −80°C 5 µM 
Rapamycin Sigma-Aldrich 553210 1 mM in DMSO −20°C 1 µM 
CCh Thermo Fisher Scientific AC10824 50 mM in dH2O −20°C 5 µM (Fig. 7), 10 M (Fig. 5), or 100 µM (Fig. 6) 
Atropine Sigma-Aldrich A0257 25 mM in dH2O 4°C 5 µM 
ATP Sigma-Aldrich 10127523001 100 mM in 100 mM MgCl2 + 200 mM Tris base −20°C 100 µM 
Histamine Thermo Fisher Scientific AC15062 100 mM in dH2O −20°C 100 µM 
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