Table 2. Biophysical parameters of... | Rockefeller University Press

Table 2.

Biophysical parameters of whole-cell calcium currents of WT and countercharge mutants of Ca_V1.1e

Constructs	N^{^a}	n^{^a}	I_Ca (pA/pF)	P value	∆	V_½ (mV)	P value	∆	TTP^a (ms)	P value	∆	τ activation (ms)	P value	∆
Ca_V1.1e	5	9	−10.7 ± 1.6	1,000	0.1	8.7 ± 1.3	<0.0001	20.3	71.2 ± 11.8	0.0001	105.6	11.6 ± 1.7	0.0001	38.9
Ca_V1.1e_E100A	7	10	−10.5 ± 1.7	1,000	0.1	−11.7 ± 1.1	<0.0001	20.3	176.8 ± 20.7	0.0001	105.6	50.3 ± 5.8	0.0001	38.9
Ca_V1.1e_D126A	4	9	−7 ± 1.2	0.218	3.7	−19.7 ± 1.4	<0.0001	28.3	131.6 ± 12.7	0.031	60.5	23.0 ± 3.0	0.016	11.6
Ca_V1.1e_E100A/D126A	6	9	−8.8 ± 1.4	0.703	8.8	−14.3 ± 0.6	<0.0001	22.9	163.4 ± 15.1	0.001	92.3	51.8 ± 6.9	0.001	40.2
Ca_V1.1e	7	14	−8.8 ± 1.3	0.777	1.0	6.6 ± 1.8	<0.0001	11.0	73.5 ± 8.6	0.315	15.7	14.0 ± 2.4	>0.9999	2.0
Ca_V1.1e_N123A	3	11	−9.8 ± 0.9	0.777	1.0	−4.3 ± 1.7	<0.0001	11.0	89 ± 4.5	0.315	15.7	16.0 ± 1.7	>0.9999	2.0
Ca_V1.1e_E100A/N123A/D126A	5	14	−7.4 ± 1.1	0.589	1.4	−12.2 ± 1	<0.0001	18.9	148.9 ± 9	<0.0001	75.5	43.8 ± 2.4	<0.0001	29.8
Ca_V1.1e	3	9	−10.8 ± 1.4	0.965	0.4	6.7 ± 1.3	<0.0001	14.3	82.5 ± 11.4	0.0009	67.2	15.4 ± 3.0	<0.0001	43.4
Ca_V1.1e_E100A/N123A	4	9	−10.4 ± 1.8	0.965	0.4	−14.3 ± 1.8	<0.0001	14.3	149.7 ± 9.5	0.0009	67.2	43.4 ± 2.3	<0.0001	43.4
Ca_V1.1e_N123A/D126A	4	10	−6.4 ± 0.7	0.047	4.4	−18.3 ± 1.3	<0.0001	18.3	153.4 ± 13	0.0004	71.0	31.2 ± 5.4	0.0149	31.3
Ca_V1.1e	3	8	−12.1 ± 2.0	0.014	4.8	5.9 ± 1.5	0.005	6.5	86.5 ± 11.7	0.012	30.4	15.4 ± 2.7	0.164	3.8
Ca_V1.1e_E140A	4	13	−7.3 ± 0.7	0.014	4.8	12.4 ± 1.3	0.005	6.5	56.0 ± 4.8	0.012	30.4	11.7 ± 1.2	0.164	3.8

Constructs	N^a	n^a	I_Ca (pA/pF)	P value	∆	V_½ (mV)	P value	∆	TTPa (ms)	P value	∆	τ activation (ms)	P value	∆
Ca_V1.1e	5	9	−10.7 ± 1.6	1,000	0.1	8.7 ± 1.3	<0.0001	20.3	71.2 ± 11.8	0.0001	105.6	11.6 ± 1.7	0.0001	38.9
Ca_V1.1e_E100A	7	10	−10.5 ± 1.7	1,000	0.1	−11.7 ± 1.1	<0.0001	20.3	176.8 ± 20.7	0.0001	105.6	50.3 ± 5.8	0.0001	38.9
Ca_V1.1e_D126A	4	9	−7 ± 1.2	0.218	3.7	−19.7 ± 1.4	<0.0001	28.3	131.6 ± 12.7	0.031	60.5	23.0 ± 3.0	0.016	11.6
Ca_V1.1e_E100A/D126A	6	9	−8.8 ± 1.4	0.703	8.8	−14.3 ± 0.6	<0.0001	22.9	163.4 ± 15.1	0.001	92.3	51.8 ± 6.9	0.001	40.2
Ca_V1.1e	7	14	−8.8 ± 1.3	0.777	1.0	6.6 ± 1.8	<0.0001	11.0	73.5 ± 8.6	0.315	15.7	14.0 ± 2.4	>0.9999	2.0
Ca_V1.1e_N123A	3	11	−9.8 ± 0.9	0.777	1.0	−4.3 ± 1.7	<0.0001	11.0	89 ± 4.5	0.315	15.7	16.0 ± 1.7	>0.9999	2.0
Ca_V1.1e_E100A/N123A/D126A	5	14	−7.4 ± 1.1	0.589	1.4	−12.2 ± 1	<0.0001	18.9	148.9 ± 9	<0.0001	75.5	43.8 ± 2.4	<0.0001	29.8
Ca_V1.1e	3	9	−10.8 ± 1.4	0.965	0.4	6.7 ± 1.3	<0.0001	14.3	82.5 ± 11.4	0.0009	67.2	15.4 ± 3.0	<0.0001	43.4
Ca_V1.1e_E100A/N123A	4	9	−10.4 ± 1.8	0.965	0.4	−14.3 ± 1.8	<0.0001	14.3	149.7 ± 9.5	0.0009	67.2	43.4 ± 2.3	<0.0001	43.4
Ca_V1.1e_N123A/D126A	4	10	−6.4 ± 0.7	0.047	4.4	−18.3 ± 1.3	<0.0001	18.3	153.4 ± 13	0.0004	71.0	31.2 ± 5.4	0.0149	31.3
Ca_V1.1e	3	8	−12.1 ± 2.0	0.014	4.8	5.9 ± 1.5	0.005	6.5	86.5 ± 11.7	0.012	30.4	15.4 ± 2.7	0.164	3.8
Ca_V1.1e_E140A	4	13	−7.3 ± 0.7	0.014	4.8	12.4 ± 1.3	0.005	6.5	56.0 ± 4.8	0.012	30.4	11.7 ± 1.2	0.164	3.8

N, number of experimental repeats (cell passages and transfections); n, number of recordings; TPP, time to peak.