Table 1. Biophysical parameters of... | Rockefeller University Press

Table 1.

Biophysical parameters of whole-cell calcium currents of WT and gating charge mutants of Ca_V1.1e

Constructs	N^{^a}	n^{^a}	I_Ca (pA/pF)	P value	∆	V_½ (mV)	P value	∆	TTP^a (ms)	P value	∆	τ activation (ms)	P value	∆
Ca_V1.1e	3	8	−12.1 ± 2.0	0.277	2.5	5.9 ± 1.5	0.273	2.7	86.5 ± 11.7	<0.0001	75.9	15.4 ± 2.7	<0.0001	15.9
Ca_V1.1e_K162A	3	11	−9.7 ± 1.2	0.277	2.5	3.2 ± 1.7	0.273	2.7	10.5 ± 0.9	<0.0001	75.9	1.4 ± 0.1	<0.0001	15.9
Ca_V1.1e	4	8	−10.2 ± 1.0	<0.0001	9.0	8.0 ± 1.5	<0.0001	25.5	54.7 ± 10.1	0.247	31.0	10.5 ± 2.0	0.732	2.6
Ca_V1.1e_R165A	6	8	−1.3 ± 0.2	<0.0001	9.0	33.6 ± 2.2	<0.0001	25.5	85.6 ± 18.0	0.247	31.0	7.9 ± 1.0	0.732	2.6
Ca_V1.1e_R168A	5	10	−6.9 ± 1.0	0.045	3.4	8.0 ± 1.2	>0.9999	0.0	135 ± 8.3	0.0002	80.4	30.4 ± 2.5	<0.0001	19.9
Ca_V1.1e_R171A	6	10	−6.7 ± 1.1	0.032	3.6	3.3 ± 1.8	0.138	4.7	142.2 ± 13.0	<0.0001	87.6	26.9 ± 2.0	<0.0001	16.4
Ca_V1.1e	6	10	−10.1 ± 1.9	0.010	6.1	5.2 ± 1.3	<0.0001	22.9	90.6 ± 17.7	0.339	25.2	19.2 ± 5.0	0.305	2.0
Ca_V1.1e_R174A	4	9	−4 ± 0.5	0.010	6.1	28.1 ± 1.3	<0.0001	22.9	115.7 ± 18.5	0.339	25.2	21.1 ± 3.2	0.305	2.0
Ca_V1.1e	3	8	−8.4 ± 1.1	0.0019	4.7	7.0 ± 1.2	0.8834	1.0	84.8 ± 5.4	0.6944	7.3	14.5 ± 1.3	0.6811	1.7
Ca_V1.1e_R171Q	3	8	−3.7 ± 0.8	0.0019	4.7	8.0 ± 1.8	0.8834	1.0	92.0 ± 7.7	0.6944	7.3	16.1 ± 1.3	0.6811	1.7
Ca_V1.1e_R174Q	3	8	−2.7 ± 0.7	0.0003	5.7	33.4 ± 2.1	<0.0001	26.4	88.2 ± 13.5	0.9614	3.6	14.9 ± 2.0	0.987	0.3

Constructs	N^a	n^a	I_Ca (pA/pF)	P value	∆	V_½ (mV)	P value	∆	TTPa (ms)	P value	∆	τ activation (ms)	P value	∆
Ca_V1.1e	3	8	−12.1 ± 2.0	0.277	2.5	5.9 ± 1.5	0.273	2.7	86.5 ± 11.7	<0.0001	75.9	15.4 ± 2.7	<0.0001	15.9
Ca_V1.1e_K162A	3	11	−9.7 ± 1.2	0.277	2.5	3.2 ± 1.7	0.273	2.7	10.5 ± 0.9	<0.0001	75.9	1.4 ± 0.1	<0.0001	15.9
Ca_V1.1e	4	8	−10.2 ± 1.0	<0.0001	9.0	8.0 ± 1.5	<0.0001	25.5	54.7 ± 10.1	0.247	31.0	10.5 ± 2.0	0.732	2.6
Ca_V1.1e_R165A	6	8	−1.3 ± 0.2	<0.0001	9.0	33.6 ± 2.2	<0.0001	25.5	85.6 ± 18.0	0.247	31.0	7.9 ± 1.0	0.732	2.6
Ca_V1.1e_R168A	5	10	−6.9 ± 1.0	0.045	3.4	8.0 ± 1.2	>0.9999	0.0	135 ± 8.3	0.0002	80.4	30.4 ± 2.5	<0.0001	19.9
Ca_V1.1e_R171A	6	10	−6.7 ± 1.1	0.032	3.6	3.3 ± 1.8	0.138	4.7	142.2 ± 13.0	<0.0001	87.6	26.9 ± 2.0	<0.0001	16.4
Ca_V1.1e	6	10	−10.1 ± 1.9	0.010	6.1	5.2 ± 1.3	<0.0001	22.9	90.6 ± 17.7	0.339	25.2	19.2 ± 5.0	0.305	2.0
Ca_V1.1e_R174A	4	9	−4 ± 0.5	0.010	6.1	28.1 ± 1.3	<0.0001	22.9	115.7 ± 18.5	0.339	25.2	21.1 ± 3.2	0.305	2.0
Ca_V1.1e	3	8	−8.4 ± 1.1	0.0019	4.7	7.0 ± 1.2	0.8834	1.0	84.8 ± 5.4	0.6944	7.3	14.5 ± 1.3	0.6811	1.7
Ca_V1.1e_R171Q	3	8	−3.7 ± 0.8	0.0019	4.7	8.0 ± 1.8	0.8834	1.0	92.0 ± 7.7	0.6944	7.3	16.1 ± 1.3	0.6811	1.7
Ca_V1.1e_R174Q	3	8	−2.7 ± 0.7	0.0003	5.7	33.4 ± 2.1	<0.0001	26.4	88.2 ± 13.5	0.9614	3.6	14.9 ± 2.0	0.987	0.3

N, number of experimental repeats (cell passages and transfections); n, number of recordings; TPP, time to peak.